##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

AclI        AflII       AgeI        AhdI        ApaI        
AscI        AspEI       AviII       AvrII       BbrPI       
BbvCI       BcgI        BfrI        BlnI        BspHI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
CpoI        DrdI        Eam1105I    EarI        EcoRV       
FspI        Ksp632I     KspI        MfeI        MluI        
MunI        NheI        NotI        NruI        NspV        
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SapI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII        1055  GACGTC               GACGT^C
AccI         4653  GTCGAC               GT^[AC][GT]AC
AccIII       1387  TCCGGA               T^CCGGA
AocI            6  CCTGAGG              CC^T[ACGT]AGG
Asp700I      4020  GAACTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          302  TGGCCA               TGG^CCA
BamHI        2374  GGATCC               G^GATCC
BbeI         4506  GGCGCC               GGCGC^C
BglI         5364  GCCCCGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        3815  TACGTG               [CT]AC^GT[GA]
BsaBI        5105  GATGCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1387  TCCGGA               T^CCGGA
BspEI        1387  TCCGGA               T^CCGGA
BsrBI        3933  GAGCGG               CCGCTC(-3/-3)
BstEII        351  GGTGACC              G^GT[ACGT]ACC
BstPI         351  GGTGACC              G^GT[ACGT]ACC
BstXI        3109  CCAGCACTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I          6  CCTGAGG              CC^T[ACGT]AGG
EagI         2172  CGGCCG               C^GGCCG
EclXI        2172  CGGCCG               C^GGCCG
Eco52I       2172  CGGCCG               C^GGCCG
Eco81I          6  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       351  GGTGACC              G^GT[ACGT]ACC
FseI         2184  GGCCGGCC             GGCCGG^CC
KasI         4506  GGCGCC               G^GCGCC
MamI         5105  GATGCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         302  TGGCCA               TGG^CCA
MroI         1387  TCCGGA               T^CCGGA
MscI          302  TGGCCA               TGG^CCA
NarI         4506  GGCGCC               GG^CGCC
PvuII        4675  CAGCTG               CAG^CTG
SacI         4667  GAGCTC               GAGCT^C
SalI         4653  GTCGAC               G^TCGAC
SbfI         2865  CCTGCAGG             CCTGCA^GG
ScaI          515  AGTACT               AGT^ACT
SexAI        5137  ACCTGGT              A^CC[AT]GGT
SfoI         4506  GGCGCC               GGC^GCC
SpeI         4191  ACTAGT               A^CTAGT
Sse8387I     2865  CCTGCAGG             CCTGCA^GG
SwaI         4203  ATTTAAAT             ATTT^AAAT
XbaI         2669  TCTAGA               T^CTAGA
XmnI         4020  GAACTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Acc65I        182  GGTACC               G^GTACC
             3875  GGTACC              
Alw44I       3086  GTGCAC               G^TGCAC
             3818  GTGCAC              
ApaLI        3086  GTGCAC               G^TGCAC
             3818  GTGCAC              
Asp718I       182  GGTACC               G^GTACC
             3875  GGTACC              
AspI         1127  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1580  GACACAGTC           
BciVI        1256  GTATCC               GTATCC(6/5)
             5322  GTATCC              
BclI           50  TGATCA               T^GATCA
             1114  TGATCA              
BglII         684  AGATCT               A^GATCT
             3741  AGATCT              
BlpI          647  GCTCAGC              GC^T[ACGT]AGC
             4219  GCTAAGC             
Bpu1102I      647  GCTCAGC              GC^T[ACGT]AGC
             4219  GCTAAGC             
BsaI         2161  GAGACC               GGTCTC(1/5)
             1778  GGTCTC              
BsiWI        1825  CGTACG               C^GTACG
             2263  CGTACG              
BsmI          368  GAATGC               GAATGC(1/-1)
             2578  GAATGC              
BspDI        1369  ATCGAT               AT^CGAT
             2429  ATCGAT              
BstAPI        255  GCAAGCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4691  GCAGCGCGTGC         
CelII         647  GCTCAGC              GC^T[ACGT]AGC
             4219  GCTAAGC             
ClaI         1369  ATCGAT               AT^CGAT
             2429  ATCGAT              
EcoRI         534  GAATTC               G^AATTC
             1529  GAATTC              
FbaI           50  TGATCA               T^GATCA
             1114  TGATCA              
HindIII      1616  AAGCTT               A^AGCTT
             5528  AAGCTT              
HpaI         2294  GTTAAC               GTT^AAC
             5010  GTTAAC              
KpnI          182  GGTACC               GGTAC^C
             3875  GGTACC              
NcoI         2203  CCATGG               C^CATGG
             3606  CCATGG              
NdeI         1447  CATATG               CA^TATG
             3864  CATATG              
PaeR7I       4459  CTCGAG               C^TCGAG
             5485  CTCGAG              
PflFI        1127  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1580  GACACAGTC           
SphI         1298  GCATGC               GCATG^C
             5348  GCATGC              
Tth111I      1127  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1580  GACACAGTC           
XcmI          308  CCACGCAGACCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4548  CCAGTGAGTCCTTGG     
XhoI         4459  CTCGAG               C^TCGAG
             5485  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  133 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       Aor51HI     
ApoI        AseI        AsnI        AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaHI       BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraI        DraII       DraIII      
DsaI        EaeI        Eco47III    EcoNI       EcoO109I    
EcoRII      EcoT14I     EcoT22I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       NsiI        
NspI        PacI        PflMI       PleI        PpuMI       
PshBI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
SspI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII