##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Aor51HI AscI AspEI AspI AviII
AvrII BbeI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaWI BseAI
BsiEI BspDI BspEI BspLU11I BsrBI
BsrDI BssHII Bst1107I BstZ17I CelII
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FbaI
FseI FspI HpaI KasI MfeI
MluI MroI MunI NarI NheI
NotI NsiI PacI PflFI PinAI
PmeI Psp1406I PvuI RsrII SbfI
SfoI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AseI 7184 ATTAAT AT^TAAT
AsnI 7184 ATTAAT AT^TAAT
Asp700I 5101 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BglI 1848 GCCCCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 755 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 7564 CGTACG C^GTACG
BssSI 4872 CACGAG CACGAG(-5/-1)
BstAPI 5679 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 5108 TTCGAA TT^CGAA
BstEII 3864 GGTGACC G^GT[ACGT]ACC
BstPI 3864 GGTGACC G^GT[ACGT]ACC
DraIII 9113 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 3864 GGTGACC G^GT[ACGT]ACC
HaeII 6622 GGCGCT [GA]GCGC^[CT]
HincII 2715 GTCGAC GT[CT]^[GA]AC
HindII 2715 GTCGAC GT[CT]^[GA]AC
KspI 8317 CCGCGG CCGC^GG
MamI 755 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 8474 CATATG CA^TATG
NruI 5063 TCGCGA TCG^CGA
NspV 5108 TTCGAA TT^CGAA
PaeR7I 669 CTCGAG C^TCGAG
PshBI 7184 ATTAAT AT^TAAT
SacI 600 GAGCTC GAGCT^C
SacII 8317 CCGCGG CCGC^GG
SalI 2715 GTCGAC G^TCGAC
SapI 5787 GAAGAGC GCTCTTC(1/4)
SexAI 5404 ACCAGGT A^CC[AT]GGT
SfiI 1847 GGCCCCGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5108 TTCGAA TT^CGAA
SmaI 3830 CCCGGG CCC^GGG
SphI 6607 GCATGC GCATG^C
SspI 7275 AATATT AAT^ATT
XhoI 669 CTCGAG C^TCGAG
XmaCI 3830 CCCGGG C^CCGGG
XmaI 3830 CCCGGG C^CCGGG
XmnI 5101 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
Acc65I 4190 GGTACC G^GTACC
7190 GGTACC
AflIII 2500 ACGTGT A^C[GA][CT]GT
4288 ACGTGT
Asp718I 4190 GGTACC G^GTACC
7190 GGTACC
BsmBI 4803 GAGACG CGTCTC(1/5)
6148 GAGACG
BspHI 5036 TCATGA T^CATGA
5051 TCATGA
EcoRI 5567 GAATTC G^AATTC
8658 GAATTC
KpnI 4190 GGTACC GGTAC^C
7190 GGTACC
PshAI 3779 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4805 GACGGATGTC
RcaI 5036 TCATGA T^CATGA
5051 TCATGA
ScaI 6568 AGTACT AGT^ACT
7101 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbrPI BbsI BbvCI
BbvI BcgI BcnI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII