##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AatII       AccI        AclI        AcyI        Alw44I      
ApaLI       AscI        AseI        AsnI        AspI        
AviII       BbeI        BbrPI       BcgI        BsaHI       
BsiWI       Bsp1407I    BspDI       BsrGI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraIII      
EagI        EclXI       Eco52I      FseI        FspI        
Hin1I       KasI        KspI        MluI        NarI        
NdeI        NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SfiI        SfoI        
SfuI        SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SwaI        Tth111I     XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AflII        1346  CTTAAG               C^TTAAG
AgeI          936  ACCGGT               A^CCGGT
AhdI         4064  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      2685  AGCGCT               AGC^GCT
AspEI        4064  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcnI          402  CCGGG                CC^[GC]GG
BfrI         1346  CTTAAG               C^TTAAG
BlpI         4744  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     4744  GCTGAGC              GC^T[ACGT]AGC
BsaBI        3879  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         648  CGGTCG               CG[GA][CT]^CG
BsmBI        4089  CGTCTC               CGTCTC(1/5)
BspHI        4418  TCATGA               T^CATGA
BspLU11I     4906  ACATGT               A^CATGT
BssHII          3  GCGCGC               G^CGCGC
BssSI        4922  CACGAG               CACGAG(-5/-1)
CelII        4744  GCTGAGC              GC^T[ACGT]AGC
Eam1105I     4064  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2685  AGCGCT               AGC^GCT
HgaI           39  GCGTC                GACGC(5/10)
HincII       1649  GTTAAC               GT[CT]^[GA]AC
HindII       1649  GTTAAC               GT[CT]^[GA]AC
HpaI         1649  GTTAAC               GTT^AAC
MamI         3879  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         3857  GCCGGC               GCC^GGC
NciI          402  CCGGG                CC^[GC]GG
NgoMIV       3857  GCCGGC               G^CCGGC
PinAI         936  ACCGGT               A^CCGGT
RcaI         4418  TCATGA               T^CATGA
SacI         2881  GAGCTC               GAGCT^C
ScaI         4537  AGTACT               AGT^ACT
SexAI        2255  ACCTGGT              A^CC[AT]GGT
SgrAI         935  CACCGGTG             C[GA]^CCGG[CT]G
SspI         4487  AATATT               AAT^ATT
TaqI         3645  TCGA                 T^CGA
TthHB8I      3645  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII           2  CGCG                 CG^CG
                4  CGCG                
AccIII         56  TCCGGA               T^CCGGA
             6621  TCCGGA              
AflIII       3160  ACGTGT               A^C[GA][CT]GT
             4906  ACATGT              
AspHI        2881  GAGCTC               G[AT]GC[AT]^C
             3718  GTGCTC              
BciVI         544  GTATCC               GTATCC(6/5)
             3364  GTATCC              
BclI         6586  TGATCA               T^GATCA
             6741  TGATCA              
BglI         2927  GCCTCCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             5646  GCCAAGGGGGC         
BglII        3998  AGATCT               A^GATCT
             5150  AGATCT              
BseAI          56  TCCGGA               T^CCGGA
             6621  TCCGGA              
BsgI         4965  CTGCAC               GTGCAG(16/14)
              164  GTGCAG              
BsiHKAI      2881  GAGCTC               G[AT]GC[AT]^C
             3718  GTGCTC              
BsmI          307  GCATTC               GAATGC(1/-1)
              666  GCATTC              
BspEI          56  TCCGGA               T^CCGGA
             6621  TCCGGA              
BsrBI        1566  GAGCGG               CCGCTC(-3/-3)
             3940  GAGCGG              
BstUI           2  CGCG                 CG^CG
                4  CGCG                
DrdI         1880  GACTCACCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2620  GACGAGGAGGTC        
EcoRI        4269  GAATTC               G^AATTC
             5415  GAATTC              
EcoRV        5834  GATATC               GAT^ATC
             5970  GATATC              
EcoT22I      1012  ATGCAT               ATGCA^T
             4398  ATGCAT              
FbaI         6586  TGATCA               T^GATCA
             6741  TGATCA              
HaeII        2246  AGCGCC               [GA]GCGC^[CT]
             2685  AGCGCT              
HindIII      3902  AAGCTT               A^AGCTT
             5518  AAGCTT              
MfeI         3303  CAATTG               C^AATTG
             5426  CAATTG              
MroI           56  TCCGGA               T^CCGGA
             6621  TCCGGA              
MunI         3303  CAATTG               C^AATTG
             5426  CAATTG              
MvnI            2  CGCG                 CG^CG
                4  CGCG                
NsiI         1012  ATGCAT               ATGCA^T
             4398  ATGCAT              


#########################################
# 3 OR MORE CUT ENZYMES (  129 enzymes) #
#########################################

Acc65I      AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        ApoI        
Asp700I     Asp718I     AvaI        AvaII       AvrII       
BalI        BamHI       BanI        BanII       BbsI        
BbvCI       BbvI        BfaI        BlnI        BmyI        
BpmI        BpuAI       BsaAI       BsaI        BsaJI       
BsaWI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrDI       
BsrFI       BsrI        BssKI       BstAPI      BstEII      
BstF5I      BstNI       BstPI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        KpnI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PshAI       
PstI        PvuII       RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SphI        StuI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Van91I      
XbaI        XcmI        XhoII       XmnI