################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII AccI AclI AcyI Alw44I ApaLI AscI AseI AsnI AspI AviII BbeI BbrPI BcgI BsaHI BsiWI Bsp1407I BspDI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraIII EagI EclXI Eco52I FseI FspI Hin1I KasI KspI MluI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AflII 1346 CTTAAG C^TTAAG AgeI 936 ACCGGT A^CCGGT AhdI 4064 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 2685 AGCGCT AGC^GCT AspEI 4064 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BcnI 402 CCGGG CC^[GC]GG BfrI 1346 CTTAAG C^TTAAG BlpI 4744 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4744 GCTGAGC GC^T[ACGT]AGC BsaBI 3879 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 648 CGGTCG CG[GA][CT]^CG BsmBI 4089 CGTCTC CGTCTC(1/5) BspHI 4418 TCATGA T^CATGA BspLU11I 4906 ACATGT A^CATGT BssHII 3 GCGCGC G^CGCGC BssSI 4922 CACGAG CACGAG(-5/-1) CelII 4744 GCTGAGC GC^T[ACGT]AGC Eam1105I 4064 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2685 AGCGCT AGC^GCT HgaI 39 GCGTC GACGC(5/10) HincII 1649 GTTAAC GT[CT]^[GA]AC HindII 1649 GTTAAC GT[CT]^[GA]AC HpaI 1649 GTTAAC GTT^AAC MamI 3879 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 3857 GCCGGC GCC^GGC NciI 402 CCGGG CC^[GC]GG NgoMIV 3857 GCCGGC G^CCGGC PinAI 936 ACCGGT A^CCGGT RcaI 4418 TCATGA T^CATGA SacI 2881 GAGCTC GAGCT^C ScaI 4537 AGTACT AGT^ACT SexAI 2255 ACCTGGT A^CC[AT]GGT SgrAI 935 CACCGGTG C[GA]^CCGG[CT]G SspI 4487 AATATT AAT^ATT TaqI 3645 TCGA T^CGA TthHB8I 3645 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 2 CGCG CG^CG 4 CGCG AccIII 56 TCCGGA T^CCGGA 6621 TCCGGA AflIII 3160 ACGTGT A^C[GA][CT]GT 4906 ACATGT AspHI 2881 GAGCTC G[AT]GC[AT]^C 3718 GTGCTC BciVI 544 GTATCC GTATCC(6/5) 3364 GTATCC BclI 6586 TGATCA T^GATCA 6741 TGATCA BglI 2927 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5646 GCCAAGGGGGC BglII 3998 AGATCT A^GATCT 5150 AGATCT BseAI 56 TCCGGA T^CCGGA 6621 TCCGGA BsgI 4965 CTGCAC GTGCAG(16/14) 164 GTGCAG BsiHKAI 2881 GAGCTC G[AT]GC[AT]^C 3718 GTGCTC BsmI 307 GCATTC GAATGC(1/-1) 666 GCATTC BspEI 56 TCCGGA T^CCGGA 6621 TCCGGA BsrBI 1566 GAGCGG CCGCTC(-3/-3) 3940 GAGCGG BstUI 2 CGCG CG^CG 4 CGCG DrdI 1880 GACTCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2620 GACGAGGAGGTC EcoRI 4269 GAATTC G^AATTC 5415 GAATTC EcoRV 5834 GATATC GAT^ATC 5970 GATATC EcoT22I 1012 ATGCAT ATGCA^T 4398 ATGCAT FbaI 6586 TGATCA T^GATCA 6741 TGATCA HaeII 2246 AGCGCC [GA]GCGC^[CT] 2685 AGCGCT HindIII 3902 AAGCTT A^AGCTT 5518 AAGCTT MfeI 3303 CAATTG C^AATTG 5426 CAATTG MroI 56 TCCGGA T^CCGGA 6621 TCCGGA MunI 3303 CAATTG C^AATTG 5426 CAATTG MvnI 2 CGCG CG^CG 4 CGCG NsiI 1012 ATGCAT ATGCA^T 4398 ATGCAT ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### Acc65I AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI Asp700I Asp718I AvaI AvaII AvrII BalI BamHI BanI BanII BbsI BbvCI BbvI BfaI BlnI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XbaI XcmI XhoII XmnI