##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII BcgI
BclI BfrI BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FbaI FseI
FspI HpaI KpnI MamI MfeI
MroI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AgeI 914 ACCGGT A^CCGGT
Alw44I 3982 GTGCAC G^TGCAC
ApaI 847 GGGCCC GGGCC^C
ApaLI 3982 GTGCAC G^TGCAC
AvrII 2962 CCTAGG C^CTAGG
BalI 769 TGGCCA TGG^CCA
BbeI 2686 GGCGCC GGCGC^C
BbsI 3971 GTCTTC GAAGAC(2/6)
BciVI 565 GGATAC GTATCC(6/5)
BglII 4484 AGATCT A^GATCT
BlnI 2962 CCTAGG C^CTAGG
BpuAI 3971 GTCTTC GAAGAC(2/6)
BsiEI 687 CGACCG CG[GA][CT]^CG
BsrBI 3937 CCGCTC CCGCTC(-3/-3)
BssHII 319 GCGCGC G^CGCGC
BstEII 4645 GGTCACC G^GT[ACGT]ACC
BstPI 4645 GGTCACC G^GT[ACGT]ACC
DrdI 1823 GACAAATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4645 GGTCACC G^GT[ACGT]ACC
HaeII 2686 GGCGCC [GA]GCGC^[CT]
HincII 2609 GTTGAC GT[CT]^[GA]AC
HindII 2609 GTTGAC GT[CT]^[GA]AC
HindIII 4376 AAGCTT A^AGCTT
KasI 2686 GGCGCC G^GCGCC
MluI 4 ACGCGT A^CGCGT
MluNI 769 TGGCCA TGG^CCA
MscI 769 TGGCCA TGG^CCA
NarI 2686 GGCGCC GG^CGCC
PinAI 914 ACCGGT A^CCGGT
PshAI 2060 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 3473 GAAGAGC GCTCTTC(1/4)
SexAI 153 ACCAGGT A^CC[AT]GGT
SfoI 2686 GGCGCC GGC^GCC
SmaI 325 CCCGGG CCC^GGG
StuI 4444 AGGCCT AGG^CCT
XmaCI 325 CCCGGG C^CCGGG
XmaI 325 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 3208 AAATTC [GA]^AATT[CT]
3232 GAATTT
AocI 2690 CCTGAGG CC^T[ACGT]AGG
5229 CCTAAGG
ApoI 3208 AAATTC [GA]^AATT[CT]
3232 GAATTT
BamHI 1665 GGATCC G^GATCC
2265 GGATCC
BanI 2362 GGTGCC G^G[CT][GA]CC
2686 GGCGCC
BbrPI 3949 CACGTG CAC^GTG
4633 CACGTG
BlpI 993 GCTCAGC GC^T[ACGT]AGC
4802 GCTGAGC
Bpu1102I 993 GCTCAGC GC^T[ACGT]AGC
4802 GCTGAGC
BsaAI 3949 CACGTG [CT]AC^GT[GA]
4633 CACGTG
BspHI 970 TCATGA T^CATGA
4210 TCATGA
BssSI 2550 CTCGTG CACGAG(-5/-1)
5198 CTCGTG
BstAPI 723 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2637 GCAACTCCTGC
Bsu36I 2690 CCTGAGG CC^T[ACGT]AGG
5229 CCTAAGG
CelII 993 GCTCAGC GC^T[ACGT]AGC
4802 GCTGAGC
DraI 1922 TTTAAA TTT^AAA
5055 TTTAAA
DraIII 608 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2589 CACTGGGTG
Eco81I 2690 CCTGAGG CC^T[ACGT]AGG
5229 CCTAAGG
EcoT22I 1785 ATGCAT ATGCA^T
5255 ATGCAT
KspI 205 CCGCGG CCGC^GG
366 CCGCGG
NaeI 1010 GCCGGC GCC^GGC
1604 GCCGGC
NcoI 700 CCATGG C^CATGG
4457 CCATGG
NgoMIV 1010 GCCGGC G^CCGGC
1604 GCCGGC
NsiI 1785 ATGCAT ATGCA^T
5255 ATGCAT
PflMI 1062 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2562 CCATGATCTGG
PmaCI 3949 CACGTG CAC^GTG
4633 CACGTG
PmlI 3949 CACGTG CAC^GTG
4633 CACGTG
RcaI 970 TCATGA T^CATGA
4210 TCATGA
SacI 1632 GAGCTC GAGCT^C
3114 GAGCTC
SacII 205 CCGCGG CCGC^GG
366 CCGCGG
SfiI 1874 GGCCCCTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4445 GGCCTCCTTGGCC
SphI 1783 GCATGC GCATG^C
1814 GCATGC
Van91I 1062 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2562 CCATGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI Asp700I
AspHI AvaI AvaII BanII BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI