##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AclI AgeI Alw44I ApaLI
AscI AseI AsnI Asp718I BbeI
BciVI BsaBI BsiWI BspHI BssHII
BstBI CpoI EcoRV EcoT22I FseI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SfoI
SfuI SgrAI SnaBI SpeI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1557 GACGTC GACGT^C
AccIII 337 TCCGGA T^CCGGA
AcyI 1557 GACGTC G[GA]^CG[CT]C
AflII 639 CTTAAG C^TTAAG
AhdI 3965 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4059 AGCGCT AGC^GCT
AspEI 3965 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3343 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2736 CCTAGG C^CTAGG
BbrPI 2813 CACGTG CAC^GTG
BfrI 639 CTTAAG C^TTAAG
BlnI 2736 CCTAGG C^CTAGG
BsaHI 1557 GACGTC G[GA]^CG[CT]C
BseAI 337 TCCGGA T^CCGGA
BsmI 1125 GAATGC GAATGC(1/-1)
Bsp1407I 6222 TGTACA T^GTACA
BspDI 1752 ATCGAT AT^CGAT
BspEI 337 TCCGGA T^CCGGA
BspLU11I 4023 ACATGT A^CATGT
BsrBI 1422 GAGCGG CCGCTC(-3/-3)
BsrGI 6222 TGTACA T^GTACA
Bst1107I 5651 GTATAC GTA^TAC
BstZ17I 5651 GTATAC GTA^TAC
ClaI 1752 ATCGAT AT^CGAT
Eam1105I 3965 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4059 AGCGCT AGC^GCT
HaeII 4059 AGCGCT [GA]GCGC^[CT]
Hin1I 1557 GACGTC G[GA]^CG[CT]C
HincII 1449 GTCGAC GT[CT]^[GA]AC
HindII 1449 GTCGAC GT[CT]^[GA]AC
MroI 337 TCCGGA T^CCGGA
NcoI 417 CCATGG C^CATGG
NdeI 5786 CATATG CA^TATG
PaeR7I 2652 CTCGAG C^TCGAG
PflFI 3343 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2813 CACGTG CAC^GTG
PmlI 2813 CACGTG CAC^GTG
PshAI 786 GACGTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1449 GTCGAC G^TCGAC
SbfI 4647 CCTGCAGG CCTGCA^GG
ScaI 1465 AGTACT AGT^ACT
SexAI 1937 ACCTGGT A^CC[AT]GGT
Sse8387I 4647 CCTGCAGG CCTGCA^GG
SspBI 6222 TGTACA T^GTACA
Tth111I 3343 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2652 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BbsI 2057 GAAGAC GAAGAC(2/6)
828 GTCTTC
BclI 349 TGATCA T^GATCA
3140 TGATCA
BglII 1072 AGATCT A^GATCT
5262 AGATCT
BpuAI 2057 GAAGAC GAAGAC(2/6)
828 GTCTTC
BsaAI 1285 CACGTA [CT]AC^GT[GA]
2813 CACGTG
BsaWI 337 TCCGGA [AT]^CCGG[AT]
5180 TCCGGT
BsiEI 2766 CGGCCG CG[GA][CT]^CG
3426 CGGCCG
BsmBI 5619 CGTCTC CGTCTC(1/5)
738 GAGACG
BssSI 5622 CTCGTG CACGAG(-5/-1)
6422 CTCGTG
BstXI 2258 CCAAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5850 CCATGCCCTTGG
DrdI 2247 GACTCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4394 GACAAATGTGTC
EagI 2766 CGGCCG C^GGCCG
3426 CGGCCG
EclXI 2766 CGGCCG C^GGCCG
3426 CGGCCG
Eco52I 2766 CGGCCG C^GGCCG
3426 CGGCCG
EcoRI 2703 GAATTC G^AATTC
3792 GAATTC
FbaI 349 TGATCA T^GATCA
3140 TGATCA
HgaI 740 GACGC GACGC(5/10)
1165 GCGTC
NaeI 1406 GCCGGC GCC^GGC
2296 GCCGGC
NgoMIV 1406 GCCGGC G^CCGGC
2296 GCCGGC
SapI 4049 GAAGAGC GCTCTTC(1/4)
1580 GCTCTTC
SfiI 5860 GGCCTCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5869 GGCCACATTGGCC
SmaI 2500 CCCGGG CCC^GGG
3564 CCCGGG
XcmI 1086 CCAGCCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5243 CCAGGTCAGAGGTGG
XmaCI 2500 CCCGGG C^CCGGG
3564 CCCGGG
XmaI 2500 CCCGGG C^CCGGG
3564 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspHI AvaI
AvaII AviII BalI BamHI BanI
BanII BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI