##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AscI AseI AsnI Asp700I Asp718I
AviII BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BsaAI BsaBI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraI DraIII EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MseI MspA1I MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 487 GTCTAC GT^[AC][GT]AC
AcsI 282 GAATTC [GA]^AATT[CT]
AgeI 694 ACCGGT A^CCGGT
AhdI 130 GACTCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 29 AGCGCT AGC^GCT
ApoI 282 GAATTC [GA]^AATT[CT]
AspEI 130 GACTCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2214 CCTAGG C^CTAGG
BalI 1526 TGGCCA TGG^CCA
BbeI 1102 GGCGCC GGCGC^C
BlnI 2214 CCTAGG C^CTAGG
BsaWI 694 ACCGGT [AT]^CCGG[AT]
BsiEI 623 CGGCCG CG[GA][CT]^CG
BsmBI 2166 CGTCTC CGTCTC(1/5)
BspLU11I 1158 ACATGT A^CATGT
EagI 623 CGGCCG C^GGCCG
Eam1105I 130 GACTCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 623 CGGCCG C^GGCCG
Eco47III 29 AGCGCT AGC^GCT
Eco52I 623 CGGCCG C^GGCCG
EcoRI 282 GAATTC G^AATTC
HincII 349 GTTGAC GT[CT]^[GA]AC
HindII 349 GTTGAC GT[CT]^[GA]AC
KasI 1102 GGCGCC G^GCGCC
MluNI 1526 TGGCCA TGG^CCA
MscI 1526 TGGCCA TGG^CCA
NaeI 1039 GCCGGC GCC^GGC
NarI 1102 GGCGCC GG^CGCC
NgoMIV 1039 GCCGGC G^CCGGC
PaeR7I 2432 CTCGAG C^TCGAG
PinAI 694 ACCGGT A^CCGGT
PshAI 1897 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2272 CTGCAG CTGCA^G
SfcI 2272 CTGCAG C^T[GA][CT]AG
SfoI 1102 GGCGCC GGC^GCC
SmlI 2432 CTCGAG C^T[CT][GA]AG
StuI 1484 AGGCCT AGG^CCT
TfiI 951 GAATC G^A[AT]TC
XbaI 1732 TCTAGA T^CTAGA
XhoI 2432 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1158 ACATGT A^C[GA][CT]GT
2172 ACACGT
Alw44I 1248 GTGCAC G^TGCAC
2146 GTGCAC
AocI 81 CCTGAGG CC^T[ACGT]AGG
339 CCTGAGG
ApaLI 1248 GTGCAC G^TGCAC
2146 GTGCAC
BamHI 542 GGATCC G^GATCC
740 GGATCC
BfaI 1733 CTAG C^TAG
2215 CTAG
BglI 625 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
675 GCCCTCCCGGC
BlpI 603 GCTGAGC GC^T[ACGT]AGC
877 GCTGAGC
Bpu1102I 603 GCTGAGC GC^T[ACGT]AGC
877 GCTGAGC
BsrBI 466 CCGCTC CCGCTC(-3/-3)
2332 CCGCTC
BstXI 1529 CCAAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1899 CCAGGGTCCTGG
BstYI 542 GGATCC [GA]^GATC[CT]
740 GGATCC
Bsu36I 81 CCTGAGG CC^T[ACGT]AGG
339 CCTGAGG
CelII 603 GCTGAGC GC^T[ACGT]AGC
877 GCTGAGC
EarI 1878 CTCTTC CTCTTC(1/4)
946 GAAGAG
Eco81I 81 CCTGAGG CC^T[ACGT]AGG
339 CCTGAGG
Ksp632I 1878 CTCTTC CTCTTC(1/4)
946 GAAGAG
MaeI 1733 CTAG C^TAG
2215 CTAG
MflI 542 GGATCC [GA]^GATC[CT]
740 GGATCC
MslI 693 CACCGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2139 CACATTCGTG
NspI 1158 ACATGT [GA]CATG^[CT]
1689 ACATGC
SacI 555 GAGCTC GAGCT^C
1808 GAGCTC
SfaNI 93 GATGC GCATC(5/9)
479 GCATC
SgrAI 296 CGCCGGTG C[GA]^CCGG[CT]G
693 CACCGGTG
XcmI 815 CCAAGAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1345 CCACCGGGGTCCTGG
XhoII 542 GGATCC [GA]^GATC[CT]
740 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AspI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflFI PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SmaI StyI TaqI TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XmaCI XmaI