##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BcgI BclI BfrI BglII
BlnI BsaBI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI PvuI
RcaI RsrII SacI SalI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 895 AACGTT AA^CGTT
AcsI 756 GAATTT [GA]^AATT[CT]
Alw44I 1128 GTGCAC G^TGCAC
ApaLI 1128 GTGCAC G^TGCAC
ApoI 756 GAATTT [GA]^AATT[CT]
BbeI 1571 GGCGCC GGCGC^C
BbrPI 729 CACGTG CAC^GTG
BbsI 270 GTCTTC GAAGAC(2/6)
BbvCI 2078 CCTCAGC CCTCAGC(-5/-2)
BciVI 1594 GTATCC GTATCC(6/5)
BglI 1228 GCCCAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2867 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2867 GCTGAGC GC^T[ACGT]AGC
BpuAI 270 GTCTTC GAAGAC(2/6)
BsiEI 1060 CGGCCG CG[GA][CT]^CG
Bsp1407I 2093 TGTACA T^GTACA
BspLU11I 999 ACATGT A^CATGT
BsrDI 1780 GCAATG GCAATG(2/0)
BsrGI 2093 TGTACA T^GTACA
BstEII 2661 GGTTACC G^GT[ACGT]ACC
BstPI 2661 GGTTACC G^GT[ACGT]ACC
CelII 2867 GCTGAGC GC^T[ACGT]AGC
DrdI 2621 GACCACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1060 CGGCCG C^GGCCG
EclXI 1060 CGGCCG C^GGCCG
Eco52I 1060 CGGCCG C^GGCCG
EcoO65I 2661 GGTTACC G^GT[ACGT]ACC
KasI 1571 GGCGCC G^GCGCC
KspI 1201 CCGCGG CCGC^GG
NarI 1571 GGCGCC GG^CGCC
NdeI 703 CATATG CA^TATG
PaeR7I 1999 CTCGAG C^TCGAG
PflMI 653 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 729 CACGTG CAC^GTG
PmlI 729 CACGTG CAC^GTG
Psp1406I 895 AACGTT AA^CGTT
SacII 1201 CCGCGG CCGC^GG
SexAI 2707 ACCTGGT A^CC[AT]GGT
SfoI 1571 GGCGCC GGC^GCC
SspBI 2093 TGTACA T^GTACA
Tsp509I 757 AATT ^AATT
Van91I 653 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1999 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 438 GGCGTC G[GA]^CG[CT]C
1571 GGCGCC
AflIII 999 ACATGT A^C[GA][CT]GT
1773 ACACGT
AhdI 1485 GACTCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1876 GACACGGAGTC
AlwI 2420 GATCC GGATC(4/5)
332 GGATC
AocI 674 CCTGAGG CC^T[ACGT]AGG
2823 CCTGAGG
ApaI 129 GGGCCC GGGCC^C
2574 GGGCCC
AspEI 1485 GACTCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1876 GACACGGAGTC
BalI 223 TGGCCA TGG^CCA
1976 TGGCCA
BfaI 1408 CTAG C^TAG
1750 CTAG
BsaAI 729 CACGTG [CT]AC^GT[GA]
1774 CACGTA
BsaHI 438 GGCGTC G[GA]^CG[CT]C
1571 GGCGCC
BsmBI 278 CGTCTC CGTCTC(1/5)
1639 CGTCTC
BstYI 332 GGATCT [GA]^GATC[CT]
2419 AGATCC
Bsu36I 674 CCTGAGG CC^T[ACGT]AGG
2823 CCTGAGG
DraIII 114 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
711 CACTCGGTG
Eam1105I 1485 GACTCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1876 GACACGGAGTC
Eco81I 674 CCTGAGG CC^T[ACGT]AGG
2823 CCTGAGG
Hin1I 438 GGCGTC G[GA]^CG[CT]C
1571 GGCGCC
MaeI 1408 CTAG C^TAG
1750 CTAG
MflI 332 GGATCT [GA]^GATC[CT]
2419 AGATCC
MluNI 223 TGGCCA TGG^CCA
1976 TGGCCA
MscI 223 TGGCCA TGG^CCA
1976 TGGCCA
MseI 760 TTAA T^TAA
2924 TTAA
NaeI 937 GCCGGC GCC^GGC
2465 GCCGGC
NcoI 241 CCATGG C^CATGG
658 CCATGG
NgoMIV 937 GCCGGC G^CCGGC
2465 GCCGGC
NspI 999 ACATGT [GA]CATG^[CT]
2668 GCATGT
PshAI 1285 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2447 GACCAACGTC
SapI 1107 GAAGAGC GCTCTTC(1/4)
1822 GCTCTTC
SbfI 24 CCTGCAGG CCTGCA^GG
878 CCTGCAGG
SmlI 372 CTCAAG C^T[CT][GA]AG
1999 CTCGAG
Sse8387I 24 CCTGCAGG CCTGCA^GG
878 CCTGCAGG
StuI 913 AGGCCT AGG^CCT
1663 AGGCCT
Tru9I 760 TTAA T^TAA
2924 TTAA
XhoII 332 GGATCT [GA]^GATC[CT]
2419 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XcmI XmaCI XmaI