##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI Alw44I ApaLI AscI
Asp718I AspI AviII BbeI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BpuAI BsaHI BsgI
BsiWI BsmBI BspDI BspHI BsrFI
BssHII BstAPI BstEII BstPI BstUI
Cfr10I ClaI DraI EcoO65I EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RcaI SacII SalI SapI SbfI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 71 enzymes) #
###################################
AccIII 80 TCCGGA T^CCGGA
AhdI 29 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 45 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2445 AGCGCT AGC^GCT
ApaI 104 GGGCCC GGGCC^C
AspEI 29 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 373 CTCGGG C^[CT]CG[GA]G
AvrII 2791 CCTAGG C^CTAGG
BalI 1377 TGGCCA TGG^CCA
BamHI 368 GGATCC G^GATCC
BanI 965 GGCACC G^G[CT][GA]CC
BbrPI 2217 CACGTG CAC^GTG
BglII 2745 AGATCT A^GATCT
BlnI 2791 CCTAGG C^CTAGG
BlpI 2211 GCTCAGC GC^T[ACGT]AGC
BpmI 680 CTGGAG CTGGAG(16/14)
Bpu1102I 2211 GCTCAGC GC^T[ACGT]AGC
BsaAI 2217 CACGTG [CT]AC^GT[GA]
BsaBI 2134 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1119 GGTCTC GGTCTC(1/5)
BsaWI 80 TCCGGA [AT]^CCGG[AT]
BseAI 80 TCCGGA T^CCGGA
BsiEI 132 CGGCCG CG[GA][CT]^CG
BsmI 672 GAATGC GAATGC(1/-1)
BsoBI 373 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1087 TGTACA T^GTACA
BspEI 80 TCCGGA T^CCGGA
BspLU11I 1186 ACATGT A^CATGT
BspMI 2856 GCAGGT ACCTGC(4/8)
BsrBI 387 GAGCGG CCGCTC(-3/-3)
BsrGI 1087 TGTACA T^GTACA
BssSI 88 CTCGTG CACGAG(-5/-1)
Bst1107I 1384 GTATAC GTA^TAC
BstBI 1569 TTCGAA TT^CGAA
BstZ17I 1384 GTATAC GTA^TAC
Bsu36I 45 CCTGAGG CC^T[ACGT]AGG
CelII 2211 GCTCAGC GC^T[ACGT]AGC
CpoI 77 CGGTCCG CG^G[AT]CCG
DrdI 284 GACAGGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 132 CGGCCG C^GGCCG
Eam1105I 29 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 132 CGGCCG C^GGCCG
Eco47III 2445 AGCGCT AGC^GCT
Eco52I 132 CGGCCG C^GGCCG
Eco81I 45 CCTGAGG CC^T[ACGT]AGG
EcoNI 2637 CCTGTTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1287 GAATTC G^AATTC
EcoRV 2138 GATATC GAT^ATC
HapII 81 CCGG C^CGG
HgaI 2939 GCGTC GACGC(5/10)
HincII 2604 GTCAAC GT[CT]^[GA]AC
HindII 2604 GTCAAC GT[CT]^[GA]AC
HpaII 81 CCGG C^CGG
MamI 2134 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1377 TGGCCA TGG^CCA
MroI 80 TCCGGA T^CCGGA
MscI 1377 TGGCCA TGG^CCA
MspI 81 CCGG C^CGG
NcoI 1808 CCATGG C^CATGG
NspV 1569 TTCGAA TT^CGAA
PmaCI 2217 CACGTG CAC^GTG
PmlI 2217 CACGTG CAC^GTG
RsrII 77 CGGTCCG CG^G[AT]CCG
SacI 1949 GAGCTC GAGCT^C
ScaI 842 AGTACT AGT^ACT
SexAI 2864 ACCTGGT A^CC[AT]GGT
SfuI 1569 TTCGAA TT^CGAA
SspBI 1087 TGTACA T^GTACA
SspI 580 AATATT AAT^ATT
XbaI 1847 TCTAGA T^CTAGA
XcmI 1502 CCAGATGAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1384 GTATAC GT^[AC][GT]AC
2726 GTAGAC
AflIII 1186 ACATGT A^C[GA][CT]GT
2873 ACACGT
AlwNI 40 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
161 CAGAAGCTG
AseI 748 ATTAAT AT^TAAT
1895 ATTAAT
AsnI 748 ATTAAT AT^TAAT
1895 ATTAAT
BseRI 2806 CTCCTC GAGGAG(10/8)
2375 GAGGAG
BsrDI 2055 CATTGC GCAATG(2/0)
1860 GCAATG
BstXI 1219 CCAGAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1575 CCAACTGTGTGG
DraIII 756 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2863 CACCTGGTG
DsaI 129 CCACGG C^C[GA][CT]GG
1808 CCATGG
FauI 2359 CCCGC CCCGC(4/6)
768 GCGGG
HaeII 2354 AGCGCC [GA]GCGC^[CT]
2445 AGCGCT
MaeII 2218 ACGT A^CGT
2875 ACGT
NdeI 1083 CATATG CA^TATG
2163 CATATG
NspI 289 GCATGT [GA]CATG^[CT]
1186 ACATGT
PflMI 160 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2862 CCACCTGGTGG
PshBI 748 ATTAAT AT^TAAT
1895 ATTAAT
PstI 1065 CTGCAG CTGCA^G
1553 CTGCAG
PvuII 1646 CAGCTG CAG^CTG
2591 CAGCTG
Van91I 160 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2862 CCACCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanII
BbvI BfaI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI