##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Aor51HI     AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BbrPI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BsaAI       BsaBI       
BsaWI       BseAI       BsiWI       BspDI       BspEI       
BspLU11I    BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DraI        DraIII      Eam1105I    
Eco47III    EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KpnI        MamI        
MfeI        MluI        MroI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SapI        SbfI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        Sse8387I    SspI        
SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII         569  GACGTC               GACGT^C
AcsI          544  GAATTC               [GA]^AATT[CT]
Alw44I       2314  GTGCAC               G^TGCAC
AocI         3020  CCTGAGG              CC^T[ACGT]AGG
ApaLI        2314  GTGCAC               G^TGCAC
ApoI          544  GAATTC               [GA]^AATT[CT]
BamHI        1910  GGATCC               G^GATCC
BbeI         1120  GGCGCC               GGCGC^C
BbvCI        2217  CCTCAGC              CCTCAGC(-5/-2)
BsmI         1858  GAATGC               GAATGC(1/-1)
Bsp1407I     1086  TGTACA               T^GTACA
BspHI        1323  TCATGA               T^CATGA
BsrGI        1086  TGTACA               T^GTACA
Bsu36I       3020  CCTGAGG              CC^T[ACGT]AGG
DrdI          148  GACCCAGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1006  CGGCCG               C^GGCCG
EclXI        1006  CGGCCG               C^GGCCG
Eco52I       1006  CGGCCG               C^GGCCG
Eco81I       3020  CCTGAGG              CC^T[ACGT]AGG
EcoRI         544  GAATTC               G^AATTC
HincII       1277  GTCGAC               GT[CT]^[GA]AC
HindII       1277  GTCGAC               GT[CT]^[GA]AC
KasI         1120  GGCGCC               G^GCGCC
MseI          525  TTAA                 T^TAA
NarI         1120  GGCGCC               GG^CGCC
PflMI        1724  CCACCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         1323  TCATGA               T^CATGA
SalI         1277  GTCGAC               G^TCGAC
ScaI          843  AGTACT               AGT^ACT
SexAI         699  ACCTGGT              A^CC[AT]GGT
SfiI         2732  GGCCGGCAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1120  GGCGCC               GGC^GCC
SspBI        1086  TGTACA               T^GTACA
TaqI         1278  TCGA                 T^CGA
TfiI          300  GAATC                G^A[AT]TC
Tru9I         525  TTAA                 T^TAA
Tsp509I       545  AATT                 ^AATT
TthHB8I      1278  TCGA                 T^CGA
Van91I       1724  CCACCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2045  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         1277  GTCGAC               GT^[AC][GT]AC
             1333  GTCTAC              
Asp700I      1600  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2478  GAAGTCCTTC          
BalI          588  TGGCCA               TGG^CCA
             2804  TGGCCA              
BfaI         1393  CTAG                 C^TAG
             2046  CTAG                
BglI          102  GCCTCCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2733  GCCGGCAAGGC         
BsiEI        1006  CGGCCG               CG[GA][CT]^CG
             1156  CGACCG              
BsrBI         361  CCGCTC               CCGCTC(-3/-3)
             1431  GAGCGG              
BsrDI        1177  CATTGC               GCAATG(2/0)
             2911  CATTGC              
BsrFI        2600  GCCGGC               [GA]^CCGG[CT]
             2733  GCCGGC              
BssSI        2533  CACGAG               CACGAG(-5/-1)
              584  CTCGTG              
BstAPI        265  GCAACGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1365  GCAAGACCTGC         
BstEII        502  GGTCACC              G^GT[ACGT]ACC
             2773  GGTCACC             
BstPI         502  GGTCACC              G^GT[ACGT]ACC
             2773  GGTCACC             
BstYI        1023  AGATCC               [GA]^GATC[CT]
             1910  GGATCC              
Cfr10I       2600  GCCGGC               [GA]^CCGG[CT]
             2733  GCCGGC              
EcoO65I       502  GGTCACC              G^GT[ACGT]ACC
             2773  GGTCACC             
KspI          260  CCGCGG               CCGC^GG
             2287  CCGCGG              
MaeI         1393  CTAG                 C^TAG
             2046  CTAG                
MflI         1023  AGATCC               [GA]^GATC[CT]
             1910  GGATCC              
MluNI         588  TGGCCA               TGG^CCA
             2804  TGGCCA              
MscI          588  TGGCCA               TGG^CCA
             2804  TGGCCA              
NaeI         2600  GCCGGC               GCC^GGC
             2733  GCCGGC              
NgoMIV       2600  GCCGGC               G^CCGGC
             2733  GCCGGC              
PshAI        1550  GACGGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2767  GACAACGGTC          
SacI          181  GAGCTC               GAGCT^C
              324  GAGCTC              
SacII         260  CCGCGG               CCGC^GG
             2287  CCGCGG              
SphI          801  GCATGC               GCATG^C
              853  GCATGC              
XhoII        1023  AGATCC               [GA]^GATC[CT]
             1910  GGATCC              
XmnI         1600  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2478  GAAGTCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvI        
BcnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        StuI        StyI        
TseI        Tsp45I      TspRI       XcmI        XmaCI       
XmaI