################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiWI BspDI BspEI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII Eam1105I Eco47III EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SapI SbfI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 569 GACGTC GACGT^C AcsI 544 GAATTC [GA]^AATT[CT] Alw44I 2314 GTGCAC G^TGCAC AocI 3020 CCTGAGG CC^T[ACGT]AGG ApaLI 2314 GTGCAC G^TGCAC ApoI 544 GAATTC [GA]^AATT[CT] BamHI 1910 GGATCC G^GATCC BbeI 1120 GGCGCC GGCGC^C BbvCI 2217 CCTCAGC CCTCAGC(-5/-2) BsmI 1858 GAATGC GAATGC(1/-1) Bsp1407I 1086 TGTACA T^GTACA BspHI 1323 TCATGA T^CATGA BsrGI 1086 TGTACA T^GTACA Bsu36I 3020 CCTGAGG CC^T[ACGT]AGG DrdI 148 GACCCAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1006 CGGCCG C^GGCCG EclXI 1006 CGGCCG C^GGCCG Eco52I 1006 CGGCCG C^GGCCG Eco81I 3020 CCTGAGG CC^T[ACGT]AGG EcoRI 544 GAATTC G^AATTC HincII 1277 GTCGAC GT[CT]^[GA]AC HindII 1277 GTCGAC GT[CT]^[GA]AC KasI 1120 GGCGCC G^GCGCC MseI 525 TTAA T^TAA NarI 1120 GGCGCC GG^CGCC PflMI 1724 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1323 TCATGA T^CATGA SalI 1277 GTCGAC G^TCGAC ScaI 843 AGTACT AGT^ACT SexAI 699 ACCTGGT A^CC[AT]GGT SfiI 2732 GGCCGGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1120 GGCGCC GGC^GCC SspBI 1086 TGTACA T^GTACA TaqI 1278 TCGA T^CGA TfiI 300 GAATC G^A[AT]TC Tru9I 525 TTAA T^TAA Tsp509I 545 AATT ^AATT TthHB8I 1278 TCGA T^CGA Van91I 1724 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2045 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 1277 GTCGAC GT^[AC][GT]AC 1333 GTCTAC Asp700I 1600 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2478 GAAGTCCTTC BalI 588 TGGCCA TGG^CCA 2804 TGGCCA BfaI 1393 CTAG C^TAG 2046 CTAG BglI 102 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2733 GCCGGCAAGGC BsiEI 1006 CGGCCG CG[GA][CT]^CG 1156 CGACCG BsrBI 361 CCGCTC CCGCTC(-3/-3) 1431 GAGCGG BsrDI 1177 CATTGC GCAATG(2/0) 2911 CATTGC BsrFI 2600 GCCGGC [GA]^CCGG[CT] 2733 GCCGGC BssSI 2533 CACGAG CACGAG(-5/-1) 584 CTCGTG BstAPI 265 GCAACGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1365 GCAAGACCTGC BstEII 502 GGTCACC G^GT[ACGT]ACC 2773 GGTCACC BstPI 502 GGTCACC G^GT[ACGT]ACC 2773 GGTCACC BstYI 1023 AGATCC [GA]^GATC[CT] 1910 GGATCC Cfr10I 2600 GCCGGC [GA]^CCGG[CT] 2733 GCCGGC EcoO65I 502 GGTCACC G^GT[ACGT]ACC 2773 GGTCACC KspI 260 CCGCGG CCGC^GG 2287 CCGCGG MaeI 1393 CTAG C^TAG 2046 CTAG MflI 1023 AGATCC [GA]^GATC[CT] 1910 GGATCC MluNI 588 TGGCCA TGG^CCA 2804 TGGCCA MscI 588 TGGCCA TGG^CCA 2804 TGGCCA NaeI 2600 GCCGGC GCC^GGC 2733 GCCGGC NgoMIV 2600 GCCGGC G^CCGGC 2733 GCCGGC PshAI 1550 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2767 GACAACGGTC SacI 181 GAGCTC GAGCT^C 324 GAGCTC SacII 260 CCGCGG CCGC^GG 2287 CCGCGG SphI 801 GCATGC GCATG^C 853 GCATGC XhoII 1023 AGATCC [GA]^GATC[CT] 1910 GGATCC XmnI 1600 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2478 GAAGTCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TseI Tsp45I TspRI XcmI XmaCI XmaI