##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        BbrPI       BcgI        
BciVI       BclI        BfrI        BsaAI       BsaBI       
BsaWI       BseAI       BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspHI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DraIII      Eam1105I    Eco47III    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KpnI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspI        
SwaI        Tru9I       Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI          686  GTCTAC               GT^[AC][GT]AC
AcyI         2223  GGCGCC               G[GA]^CG[CT]C
AflIII        360  ACATGT               A^C[GA][CT]GT
AviII        1082  TGCGCA               TGC^GCA
AvrII        2710  CCTAGG               C^CTAGG
BamHI        2965  GGATCC               G^GATCC
BbeI         2223  GGCGCC               GGCGC^C
BglII         216  AGATCT               A^GATCT
BlnI         2710  CCTAGG               C^CTAGG
BsaHI        2223  GGCGCC               G[GA]^CG[CT]C
Bsp1407I     3460  TGTACA               T^GTACA
BspLU11I      360  ACATGT               A^CATGT
BspMI        1259  GCAGGT               ACCTGC(4/8)
BsrGI        3460  TGTACA               T^GTACA
BssSI         176  CTCGTG               CACGAG(-5/-1)
BstAPI        398  GCAACTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI          940  GACCCACAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2242  CGGCCG               C^GGCCG
EclXI        2242  CGGCCG               C^GGCCG
Eco52I       2242  CGGCCG               C^GGCCG
FspI         1082  TGCGCA               TGC^GCA
HgaI         1672  GACGC                GACGC(5/10)
Hin1I        2223  GGCGCC               G[GA]^CG[CT]C
KasI         2223  GGCGCC               G^GCGCC
KspI          115  CCGCGG               CCGC^GG
NarI         2223  GGCGCC               GG^CGCC
PflMI        2428  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII         115  CCGCGG               CCGC^GG
SfoI         2223  GGCGCC               GGC^GCC
SmaI           40  CCCGGG               CCC^GGG
SphI         2181  GCATGC               GCATG^C
SspBI        3460  TGTACA               T^GTACA
TaqI          282  TCGA                 T^CGA
TthHB8I       282  TCGA                 T^CGA
Van91I       2428  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1204  TCTAGA               T^CTAGA
XmaCI          40  CCCGGG               C^CCGGG
XmaI           40  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

Asp700I       469  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2095  GAAGAAGTTC          
AvaI           40  CCCGGG               C^[CT]CG[GA]G
              563  CCCGAG              
BalI          534  TGGCCA               TGG^CCA
             2187  TGGCCA              
BglI           38  GCCCCGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2266  GCCTGACCGGC         
BsaI          307  GGTCTC               GGTCTC(1/5)
             3126  GGTCTC              
BsiEI         829  CGACCG               CG[GA][CT]^CG
             2242  CGGCCG              
BsoBI          40  CCCGGG               C^[CT]CG[GA]G
              563  CCCGAG              
BsrBI        1657  CCGCTC               CCGCTC(-3/-3)
             1411  GAGCGG              
BsrDI         519  GCAATG               GCAATG(2/0)
             1590  GCAATG              
BstYI         216  AGATCT               [GA]^GATC[CT]
             2965  GGATCC              
HincII       1745  GTTGAC               GT[CT]^[GA]AC
             2675  GTTGAC              
HindII       1745  GTTGAC               GT[CT]^[GA]AC
             2675  GTTGAC              
MflI          216  AGATCT               [GA]^GATC[CT]
             2965  GGATCC              
MluNI         534  TGGCCA               TGG^CCA
             2187  TGGCCA              
MscI          534  TGGCCA               TGG^CCA
             2187  TGGCCA              
ScaI          886  AGTACT               AGT^ACT
             1302  AGTACT              
TfiI          934  GATTC                G^A[AT]TC
             3580  GAATC               
XhoII         216  AGATCT               [GA]^GATC[CT]
             2965  GGATCC              
XmnI          469  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2095  GAAGAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        AspHI       AvaII       
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TseI        Tsp45I      Tsp509I     
TspRI       XcmI