##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AocI Aor51HI ApaI AscI Asp718I
AviII BamHI BbeI BcgI BciVI
BsaBI BsaHI BsiWI BsmBI BspDI
BsrBI BsrFI BssHII Bst1107I BstBI
BstZ17I Bsu36I Cfr10I ClaI CpoI
EagI EclXI Eco47III Eco52I Eco81I
EcoRV FseI FspI HgaI Hin1I
HpaI KasI KpnI MamI MluI
NaeI NarI NgoMIV NotI NruI
NspV PacI PinAI PmeI PshAI
Psp1406I RsrII SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
Sse8387I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 5106 TCCGGA T^CCGGA
AflII 731 CTTAAG C^TTAAG
AhdI 3874 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3213 GTGCAC G^TGCAC
ApaLI 3213 GTGCAC G^TGCAC
AspEI 3874 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4039 CCTAGG C^CTAGG
BclI 2063 TGATCA T^GATCA
BfrI 731 CTTAAG C^TTAAG
BglI 1278 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4039 CCTAGG C^CTAGG
BseAI 5106 TCCGGA T^CCGGA
BsiEI 5182 CGATCG CG[GA][CT]^CG
BspEI 5106 TCCGGA T^CCGGA
BspLU11I 5236 ACATGT A^CATGT
BssSI 1148 CACGAG CACGAG(-5/-1)
BstAPI 2762 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1368 CCATTGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 4511 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 5180 GACGATCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3874 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2725 CCTCATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2063 TGATCA T^GATCA
HaeII 115 GGCGCT [GA]GCGC^[CT]
HincII 1401 GTCGAC GT[CT]^[GA]AC
HindII 1401 GTCGAC GT[CT]^[GA]AC
HindIII 4189 AAGCTT A^AGCTT
KspI 111 CCGCGG CCGC^GG
MfeI 3020 CAATTG C^AATTG
MroI 5106 TCCGGA T^CCGGA
MunI 3020 CAATTG C^AATTG
NcoI 3837 CCATGG C^CATGG
NdeI 1628 CATATG CA^TATG
NheI 2359 GCTAGC G^CTAGC
PaeR7I 977 CTCGAG C^TCGAG
PflMI 2744 CCACACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 5182 CGATCG CGAT^CG
SacI 1766 GAGCTC GAGCT^C
SacII 111 CCGCGG CCGC^GG
SalI 1401 GTCGAC G^TCGAC
SexAI 4512 ACCAGGT A^CC[AT]GGT
SnaBI 4490 TACGTA TAC^GTA
SpeI 2841 ACTAGT A^CTAGT
StuI 4368 AGGCCT AGG^CCT
SwaI 3926 ATTTAAAT ATTT^AAAT
Van91I 2744 CCACACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 4433 CCAGCTCTCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 977 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 112 CGCG CG^CG
477 CGCG
BanI 4500 GGCACC G^G[CT][GA]CC
4509 GGCACC
BbrPI 791 CACGTG CAC^GTG
3677 CACGTG
BbvCI 70 GCTGAGG CCTCAGC(-5/-2)
2666 GCTGAGG
BlpI 3611 GCTGAGC GC^T[ACGT]AGC
4420 GCTAAGC
Bpu1102I 3611 GCTGAGC GC^T[ACGT]AGC
4420 GCTAAGC
BsaI 350 GAGACC GGTCTC(1/5)
1379 GGTCTC
BspHI 390 TCATGA T^CATGA
1576 TCATGA
BstEII 537 GGTTACC G^GT[ACGT]ACC
1682 GGTGACC
BstPI 537 GGTTACC G^GT[ACGT]ACC
1682 GGTGACC
BstUI 112 CGCG CG^CG
477 CGCG
CelII 3611 GCTGAGC GC^T[ACGT]AGC
4420 GCTAAGC
CfoI 116 GCGC GCG^C
5130 GCGC
EcoO65I 537 GGTTACC G^GT[ACGT]ACC
1682 GGTGACC
EcoRI 1354 GAATTC G^AATTC
4221 GAATTC
FauI 5561 CCCGC CCCGC(4/6)
5303 GCGGG
HhaI 116 GCGC GCG^C
5130 GCGC
HinP1I 116 GCGC G^CGC
5130 GCGC
MvnI 112 CGCG CG^CG
477 CGCG
PmaCI 791 CACGTG CAC^GTG
3677 CACGTG
PmlI 791 CACGTG CAC^GTG
3677 CACGTG
PpuMI 278 GGGACCC [GA]G^G[AT]CC[CT]
4650 AGGACCC
PvuII 1280 CAGCTG CAG^CTG
1298 CAGCTG
RcaI 390 TCATGA T^CATGA
1576 TCATGA
SphI 3533 GCATGC GCATG^C
3862 GCATGC
XbaI 2269 TCTAGA T^CTAGA
4409 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AspI AvaI
AvaII BalI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflFI PleI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII
XmnI