################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AccIII AclI AscI AseI AsnI Asp700I AspI AvrII BamHI BbrPI BbvCI BcgI BlnI BsaAI BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspEI BspHI BsrBI BsrGI BssHII Bst1107I BstBI BstZ17I CpoI DraI DraIII EagI EclXI Eco52I EcoRI EcoRV FseI HpaI MamI MluI MroI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspBI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 3338 GGTACC G^GTACC AccI 4133 GTAGAC GT^[AC][GT]AC AflII 2675 CTTAAG C^TTAAG AflIII 4200 ACATGT A^C[GA][CT]GT AgeI 132 ACCGGT A^CCGGT AocI 2271 CCTAAGG CC^T[ACGT]AGG Aor51HI 1510 AGCGCT AGC^GCT Asp718I 3338 GGTACC G^GTACC AviII 1034 TGCGCA TGC^GCA BciVI 1561 GGATAC GTATCC(6/5) BclI 2608 TGATCA T^GATCA BfrI 2675 CTTAAG C^TTAAG BspDI 1111 ATCGAT AT^CGAT BspLU11I 4200 ACATGT A^CATGT BssSI 835 CACGAG CACGAG(-5/-1) BstEII 3726 GGTTACC G^GT[ACGT]ACC BstPI 3726 GGTTACC G^GT[ACGT]ACC Bsu36I 2271 CCTAAGG CC^T[ACGT]AGG ClaI 1111 ATCGAT AT^CGAT Eco47III 1510 AGCGCT AGC^GCT Eco81I 2271 CCTAAGG CC^T[ACGT]AGG EcoNI 1323 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3726 GGTTACC G^GT[ACGT]ACC FbaI 2608 TGATCA T^GATCA FspI 1034 TGCGCA TGC^GCA HincII 1153 GTCAAC GT[CT]^[GA]AC HindII 1153 GTCAAC GT[CT]^[GA]AC KpnI 3338 GGTACC GGTAC^C MfeI 2078 CAATTG C^AATTG MunI 2078 CAATTG C^AATTG NcoI 140 CCATGG C^CATGG NdeI 3185 CATATG CA^TATG PinAI 132 ACCGGT A^CCGGT ScaI 1244 AGTACT AGT^ACT SmaI 740 CCCGGG CCC^GGG SpeI 4081 ACTAGT A^CTAGT SspI 3806 AATATT AAT^ATT StuI 1682 AGGCCT AGG^CCT XbaI 3078 TCTAGA T^CTAGA XmaCI 740 CCCGGG C^CCGGG XmaI 740 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AhdI 1227 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3652 GACTGTATGTC Alw44I 1318 GTGCAC G^TGCAC 1632 GTGCAC ApaI 71 GGGCCC GGGCC^C 745 GGGCCC ApaLI 1318 GTGCAC G^TGCAC 1632 GTGCAC AspEI 1227 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3652 GACTGTATGTC AvaI 740 CCCGGG C^[CT]CG[GA]G 1673 CTCGGG BbeI 660 GGCGCC GGCGC^C 2290 GGCGCC BglI 738 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2282 GCCGTGGAGGC BsaWI 132 ACCGGT [AT]^CCGG[AT] 2009 ACCGGA BsmI 3388 GCATTC GAATGC(1/-1) 3513 GCATTC BsoBI 740 CCCGGG C^[CT]CG[GA]G 1673 CTCGGG BsrDI 867 GCAATG GCAATG(2/0) 1990 GCAATG BstAPI 99 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 854 GCATGTACTGC BstXI 52 CCAGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2928 CCAGAACCCTGG DrdI 68 GACGGGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 261 GACCCCAGAGTC Eam1105I 1227 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3652 GACTGTATGTC EcoT22I 3690 ATGCAT ATGCA^T 4231 ATGCAT KasI 660 GGCGCC G^GCGCC 2290 GGCGCC NarI 660 GGCGCC GG^CGCC 2290 GGCGCC NsiI 3690 ATGCAT ATGCA^T 4231 ATGCAT PshAI 1381 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1719 GACCCTGGTC SacI 1885 GAGCTC GAGCT^C 2337 GAGCTC SfoI 660 GGCGCC GGC^GCC 2290 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII