##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AatII       AccIII      AclI        AscI        AseI        
AsnI        Asp700I     AspI        AvrII       BamHI       
BbrPI       BbvCI       BcgI        BlnI        BsaAI       
BsaBI       BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspEI       BspHI       BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     CpoI        
DraI        DraIII      EagI        EclXI       Eco52I      
EcoRI       EcoRV       FseI        HpaI        MamI        
MluI        MroI        NaeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SapI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SphI        Sse8387I    SspBI       
SwaI        Tth111I     XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       3338  GGTACC               G^GTACC
AccI         4133  GTAGAC               GT^[AC][GT]AC
AflII        2675  CTTAAG               C^TTAAG
AflIII       4200  ACATGT               A^C[GA][CT]GT
AgeI          132  ACCGGT               A^CCGGT
AocI         2271  CCTAAGG              CC^T[ACGT]AGG
Aor51HI      1510  AGCGCT               AGC^GCT
Asp718I      3338  GGTACC               G^GTACC
AviII        1034  TGCGCA               TGC^GCA
BciVI        1561  GGATAC               GTATCC(6/5)
BclI         2608  TGATCA               T^GATCA
BfrI         2675  CTTAAG               C^TTAAG
BspDI        1111  ATCGAT               AT^CGAT
BspLU11I     4200  ACATGT               A^CATGT
BssSI         835  CACGAG               CACGAG(-5/-1)
BstEII       3726  GGTTACC              G^GT[ACGT]ACC
BstPI        3726  GGTTACC              G^GT[ACGT]ACC
Bsu36I       2271  CCTAAGG              CC^T[ACGT]AGG
ClaI         1111  ATCGAT               AT^CGAT
Eco47III     1510  AGCGCT               AGC^GCT
Eco81I       2271  CCTAAGG              CC^T[ACGT]AGG
EcoNI        1323  CCTGACTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3726  GGTTACC              G^GT[ACGT]ACC
FbaI         2608  TGATCA               T^GATCA
FspI         1034  TGCGCA               TGC^GCA
HincII       1153  GTCAAC               GT[CT]^[GA]AC
HindII       1153  GTCAAC               GT[CT]^[GA]AC
KpnI         3338  GGTACC               GGTAC^C
MfeI         2078  CAATTG               C^AATTG
MunI         2078  CAATTG               C^AATTG
NcoI          140  CCATGG               C^CATGG
NdeI         3185  CATATG               CA^TATG
PinAI         132  ACCGGT               A^CCGGT
ScaI         1244  AGTACT               AGT^ACT
SmaI          740  CCCGGG               CCC^GGG
SpeI         4081  ACTAGT               A^CTAGT
SspI         3806  AATATT               AAT^ATT
StuI         1682  AGGCCT               AGG^CCT
XbaI         3078  TCTAGA               T^CTAGA
XmaCI         740  CCCGGG               C^CCGGG
XmaI          740  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AhdI         1227  GACCACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3652  GACTGTATGTC         
Alw44I       1318  GTGCAC               G^TGCAC
             1632  GTGCAC              
ApaI           71  GGGCCC               GGGCC^C
              745  GGGCCC              
ApaLI        1318  GTGCAC               G^TGCAC
             1632  GTGCAC              
AspEI        1227  GACCACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3652  GACTGTATGTC         
AvaI          740  CCCGGG               C^[CT]CG[GA]G
             1673  CTCGGG              
BbeI          660  GGCGCC               GGCGC^C
             2290  GGCGCC              
BglI          738  GCCCCGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2282  GCCGTGGAGGC         
BsaWI         132  ACCGGT               [AT]^CCGG[AT]
             2009  ACCGGA              
BsmI         3388  GCATTC               GAATGC(1/-1)
             3513  GCATTC              
BsoBI         740  CCCGGG               C^[CT]CG[GA]G
             1673  CTCGGG              
BsrDI         867  GCAATG               GCAATG(2/0)
             1990  GCAATG              
BstAPI         99  GCACCCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              854  GCATGTACTGC         
BstXI          52  CCAGGGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2928  CCAGAACCCTGG        
DrdI           68  GACGGGCCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              261  GACCCCAGAGTC        
Eam1105I     1227  GACCACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3652  GACTGTATGTC         
EcoT22I      3690  ATGCAT               ATGCA^T
             4231  ATGCAT              
KasI          660  GGCGCC               G^GCGCC
             2290  GGCGCC              
NarI          660  GGCGCC               GG^CGCC
             2290  GGCGCC              
NsiI         3690  ATGCAT               ATGCA^T
             4231  ATGCAT              
PshAI        1381  GACTTGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1719  GACCCTGGTC          
SacI         1885  GAGCTC               GAGCT^C
             2337  GAGCTC              
SfoI          660  GGCGCC               GGC^GCC
             2290  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BglII       BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII