##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       AccIII      AcyI        AflIII      AgeI        
AocI        Aor51HI     AscI        AspI        AviII       
AvrII       BbeI        BcgI        BlnI        BsaHI       
BseAI       BsiWI       BspEI       BspLU11I    BsrFI       
BssHII      BstEII      BstPI       Bsu36I      Cfr10I      
CpoI        DrdI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRV       FseI        
FspI        HgaI        Hin1I       HpaI        KasI        
KspI        MluI        MroI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PpuMI       RsrII       SacII       SalI        SapI        
SbfI        SfiI        SfoI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SwaI        Tth111I     
Van91I      XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I        554  GGTACC               G^GTACC
AccII        3778  CGCG                 CG^CG
AclI         1413  AACGTT               AA^CGTT
AflII        5966  CTTAAG               C^TTAAG
Alw44I       3153  GTGCAC               G^TGCAC
ApaI         5447  GGGCCC               GGGCC^C
ApaLI        3153  GTGCAC               G^TGCAC
AseI          332  ATTAAT               AT^TAAT
AsnI          332  ATTAAT               AT^TAAT
Asp718I       554  GGTACC               G^GTACC
BalI         5096  TGGCCA               TGG^CCA
BamHI        4307  GGATCC               G^GATCC
BanI          554  GGTACC               G^G[CT][GA]CC
BbrPI        1796  CACGTG               CAC^GTG
BclI         1072  TGATCA               T^GATCA
BfrI         5966  CTTAAG               C^TTAAG
BlpI         2231  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2231  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1796  CACGTG               [CT]AC^GT[GA]
BsaWI        4450  ACCGGA               [AT]^CCGG[AT]
BsiEI        5000  CGATCG               CG[GA][CT]^CG
BspDI         396  ATCGAT               AT^CGAT
BsrBI        4073  CCGCTC               CCGCTC(-3/-3)
BssSI        5408  CACGAG               CACGAG(-5/-1)
BstAPI       3080  GCAACCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        3778  CGCG                 CG^CG
CelII        2231  GCTGAGC              GC^T[ACGT]AGC
ClaI          396  ATCGAT               AT^CGAT
DraIII       1172  CACGATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         5096  TGGCCA               [CT]^GGCC[GA]
EcoRI        1855  GAATTC               G^AATTC
FbaI         1072  TGATCA               T^GATCA
HaeII        5210  AGCGCC               [GA]GCGC^[CT]
HindIII      2711  AAGCTT               A^AGCTT
KpnI          554  GGTACC               GGTAC^C
MfeI         1488  CAATTG               C^AATTG
MluNI        5096  TGGCCA               TGG^CCA
MscI         5096  TGGCCA               TGG^CCA
MunI         1488  CAATTG               C^AATTG
MvnI         3778  CGCG                 CG^CG
NcoI         3825  CCATGG               C^CATGG
NspI         5798  GCATGT               [GA]CATG^[CT]
PmaCI        1796  CACGTG               CAC^GTG
PmlI         1796  CACGTG               CAC^GTG
PshAI        3332  GACCCCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         332  ATTAAT               AT^TAAT
Psp1406I     1413  AACGTT               AA^CGTT
PvuI         5000  CGATCG               CGAT^CG
SacI         2234  GAGCTC               GAGCT^C
ScaI         1432  AGTACT               AGT^ACT
SexAI         363  ACCAGGT              A^CC[AT]GGT


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AhdI         3577  GACTCAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5381  GACCCTTGGTC         
AlwNI         720  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             5473  CAGTTTCTG           
AspEI        3577  GACTCAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5381  GACCCTTGGTC         
BbvCI        3671  CCTCAGC              CCTCAGC(-5/-2)
             3698  CCTCAGC             
BsaBI        2111  GATTTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4308  GATCCAAATC          
BsgI         2063  GTGCAG               GTGCAG(16/14)
             3087  GTGCAG              
BsmBI        4581  CGTCTC               CGTCTC(1/5)
             1524  GAGACG              
BsmI          135  GAATGC               GAATGC(1/-1)
             3052  GAATGC              
Bsp1407I     2771  TGTACA               T^GTACA
             5670  TGTACA              
BspHI        1699  TCATGA               T^CATGA
             5462  TCATGA              
BsrGI        2771  TGTACA               T^GTACA
             5670  TGTACA              
Bst1107I     1364  GTATAC               GTA^TAC
             6052  GTATAC              
BstBI        3216  TTCGAA               TT^CGAA
             3565  TTCGAA              
BstXI         364  CCAGGTATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4448  CCACCGGAGTGG        
BstZ17I      1364  GTATAC               GTA^TAC
             6052  GTATAC              
DraII        2520  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             5447  GGGCCCT             
DsaI         3825  CCATGG               C^C[GA][CT]GG
             5339  CCGTGG              
Eam1105I     3577  GACTCAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5381  GACCCTTGGTC         
EcoO109I     2520  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             5447  GGGCCCT             
MamI         2111  GATTTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4308  GATCCAAATC          
NdeI         3071  CATATG               CA^TATG
             6007  CATATG              
NspV         3216  TTCGAA               TT^CGAA
             3565  TTCGAA              
RcaI         1699  TCATGA               T^CATGA
             5462  TCATGA              
SfuI         3216  TTCGAA               TT^CGAA
             3565  TTCGAA              
SmaI         4614  CCCGGG               CCC^GGG
             4717  CCCGGG              
SspBI        2771  TGTACA               T^GTACA
             5670  TGTACA              
StuI         3237  AGGCCT               AGG^CCT
             5013  AGGCCT              
XmaCI        4614  CCCGGG               C^CCGGG
             4717  CCCGGG              
XmaI         4614  CCCGGG               C^CCGGG
             4717  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AspHI       AvaI        
AvaII       BanII       BbsI        BbvI        BciVI       
BcnI        BfaI        BglI        BglII       BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
EarI        EcoNI       EcoRII      EcoT14I     EcoT22I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NsiI        PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XbaI        XhoII       XmnI