##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AccII       AclI        AgeI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        AviII       
BbeI        BbrPI       BcgI        BciVI       BsaAI       
BsiEI       BsiWI       BsmBI       BspDI       BspHI       
BssHII      BstAPI      BstBI       BstUI       ClaI        
CpoI        DrdI        EagI        EclXI       Eco47III    
Eco52I      FseI        FspI        HpaI        KasI        
KspI        MfeI        MluI        MunI        MvnI        
NaeI        NarI        NgoMIV      NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SbfI        
SfoI        SfuI        SgrAI       SnaBI       Sse8387I    
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AatII        3724  GACGTC               GACGT^C
Acc65I       2103  GGTACC               G^GTACC
AccIII       3117  TCCGGA               T^CCGGA
AhdI         2162  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I      2103  GGTACC               G^GTACC
AspEI        2162  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        4709  CCTAGG               C^CTAGG
BlnI         4709  CCTAGG               C^CTAGG
BsaBI        5731  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2527  GGTCTC               GGTCTC(1/5)
BseAI        3117  TCCGGA               T^CCGGA
BsgI         4895  GTGCAG               GTGCAG(16/14)
BspEI        3117  TCCGGA               T^CCGGA
BssSI        2556  CACGAG               CACGAG(-5/-1)
CfoI         2791  GCGC                 GCG^C
DraIII       2954  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     2162  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI          61  GAATTC               G^AATTC
EcoT22I      6642  ATGCAT               ATGCA^T
HaeII        2790  AGCGCC               [GA]GCGC^[CT]
HhaI         2791  GCGC                 GCG^C
HinP1I       2791  GCGC                 G^CGC
KpnI         2103  GGTACC               GGTAC^C
MamI         5731  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         3117  TCCGGA               T^CCGGA
NheI         1067  GCTAGC               G^CTAGC
NsiI         6642  ATGCAT               ATGCA^T
SalI         2073  GTCGAC               G^TCGAC
ScaI         2154  AGTACT               AGT^ACT
SexAI        1884  ACCTGGT              A^CC[AT]GGT
SfiI         2041  GGCCTCTGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         3896  ACTAGT               A^CTAGT
XbaI         7018  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcyI         1307  GACGCC               G[GA]^CG[CT]C
             3724  GACGTC              
AflII         245  CTTAAG               C^TTAAG
             3876  CTTAAG              
BalI         4166  TGGCCA               TGG^CCA
             6746  TGGCCA              
BclI         3048  TGATCA               T^GATCA
             7122  TGATCA              
BfrI          245  CTTAAG               C^TTAAG
             3876  CTTAAG              
BglII         340  AGATCT               A^GATCT
             1092  AGATCT              
BsaHI        1307  GACGCC               G[GA]^CG[CT]C
             3724  GACGTC              
BsmI         7022  GAATGC               GAATGC(1/-1)
             5138  GCATTC              
BsrFI        1713  ACCGGC               [GA]^CCGG[CT]
             2766  ACCGGC              
Bst1107I     6035  GTATAC               GTA^TAC
             7143  GTATAC              
BstZ17I      6035  GTATAC               GTA^TAC
             7143  GTATAC              
Cfr10I       1713  ACCGGC               [GA]^CCGG[CT]
             2766  ACCGGC              
EcoNI        2029  CCTAAGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3820  CCTTTCCCAGG         
EcoRV        1535  GATATC               GAT^ATC
             4018  GATATC              
FbaI         3048  TGATCA               T^GATCA
             7122  TGATCA              
Hin1I        1307  GACGCC               G[GA]^CG[CT]C
             3724  GACGTC              
HindIII      4313  AAGCTT               A^AGCTT
             6153  AAGCTT              
MluNI        4166  TGGCCA               TGG^CCA
             6746  TGGCCA              
MscI         4166  TGGCCA               TGG^CCA
             6746  TGGCCA              
PstI         5063  CTGCAG               CTGCA^G
             5975  CTGCAG              
SacI         6909  GAGCTC               GAGCT^C
             7037  GAGCTC              
SapI         5316  GAAGAGC              GCTCTTC(1/4)
              756  GCTCTTC             
SmaI         1978  CCCGGG               CCC^GGG
             6299  CCCGGG              
SphI         2448  GCATGC               GCATG^C
             2623  GCATGC              
SspI         3174  AATATT               AAT^ATT
             5028  AATATT              
XcmI          334  CCATGCAGATCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             6074  CCAAGATGCAACTGG     
XmaCI        1978  CCCGGG               C^CCGGG
             6299  CCCGGG              
XmaI         1978  CCCGGG               C^CCGGG
             6299  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  130 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        ApaI        
ApoI        Asp700I     AspHI       AspI        AvaI        
AvaII       BamHI       BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaJI       BsaWI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspLU11I    BspMI       BsrBI       BsrDI       
BsrGI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
Eco81I      EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PflMI       
PleI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XhoII       XmnI