##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflIII      
AgeI        Alw44I      ApaLI       AscI        AspHI       
AspI        AvrII       BbeI        BbrPI       BcgI        
BciVI       BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspLU11I    BssHII      BssSI       Bst1107I    
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
EclXI       Eco52I      EcoT22I     FseI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KspI        MamI        MfeI        MluI        
MroI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SgrAI       
SnaBI       SphI        Sse8387I    SspI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I       1329  GGTACC               G^GTACC
AflII         447  CTTAAG               C^TTAAG
Aor51HI       808  AGCGCT               AGC^GCT
ApaI          743  GGGCCC               GGGCC^C
AseI         2200  ATTAAT               AT^TAAT
AsnI         2200  ATTAAT               AT^TAAT
Asp700I      2385  GAACAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1329  GGTACC               G^GTACC
AviII          10  TGCGCA               TGC^GCA
BamHI        2502  GGATCC               G^GATCC
BclI         1805  TGATCA               T^GATCA
BfrI          447  CTTAAG               C^TTAAG
BsaAI        2179  TACGTG               [CT]AC^GT[GA]
BsgI          492  CTGCAC               GTGCAG(16/14)
Bsp1407I     1898  TGTACA               T^GTACA
BspHI        2432  TCATGA               T^CATGA
BspMI        2009  ACCTGC               ACCTGC(4/8)
BsrBI        1325  GAGCGG               CCGCTC(-3/-3)
BsrFI        1886  GCCGGC               [GA]^CCGG[CT]
BsrGI        1898  TGTACA               T^GTACA
BstBI        2094  TTCGAA               TT^CGAA
BstEII       1074  GGTGACC              G^GT[ACGT]ACC
BstPI        1074  GGTGACC              G^GT[ACGT]ACC
BstYI        2502  GGATCC               [GA]^GATC[CT]
Cfr10I       1886  GCCGGC               [GA]^CCGG[CT]
Eco47III      808  AGCGCT               AGC^GCT
EcoO65I      1074  GGTGACC              G^GT[ACGT]ACC
EcoRV        1921  GATATC               GAT^ATC
FbaI         1805  TGATCA               T^GATCA
FspI           10  TGCGCA               TGC^GCA
KpnI         1329  GGTACC               GGTAC^C
MflI         2502  GGATCC               [GA]^GATC[CT]
MslI         1068  CACTGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         1886  GCCGGC               GCC^GGC
NgoMIV       1886  GCCGGC               G^CCGGC
NspV         2094  TTCGAA               TT^CGAA
PflMI         463  CCACGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        2200  ATTAAT               AT^TAAT
RcaI         2432  TCATGA               T^CATGA
SexAI        1357  ACCAGGT              A^CC[AT]GGT
SfuI         2094  TTCGAA               TT^CGAA
SmlI          447  CTTAAG               C^T[CT][GA]AG
SpeI         2379  ACTAGT               A^CTAGT
SspBI        1898  TGTACA               T^GTACA
StuI         1782  AGGCCT               AGG^CCT
Van91I        463  CCACGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        2502  GGATCC               [GA]^GATC[CT]
XmnI         2385  GAACAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          568  GTCTAC               GT^[AC][GT]AC
             1408  GTAGAC              
AccII         505  CGCG                 CG^CG
             1654  CGCG                
AocI          242  CCTTAGG              CC^T[ACGT]AGG
              871  CCTAAGG             
BalI          611  TGGCCA               TGG^CCA
             1847  TGGCCA              
BbsI         1592  GAAGAC               GAAGAC(2/6)
              306  GTCTTC              
BpuAI        1592  GAAGAC               GAAGAC(2/6)
              306  GTCTTC              
BsaI          590  GAGACC               GGTCTC(1/5)
             1567  GAGACC              
BstUI         505  CGCG                 CG^CG
             1654  CGCG                
Bsu36I        242  CCTTAGG              CC^T[ACGT]AGG
              871  CCTAAGG             
EaeI          611  TGGCCA               [CT]^GGCC[GA]
             1847  TGGCCA              
Eco81I        242  CCTTAGG              CC^T[ACGT]AGG
              871  CCTAAGG             
EcoNI         219  CCTGGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1491  CCTGGCAGAGG         
EcoRI         229  GAATTC               G^AATTC
             2266  GAATTC              
HaeII         808  AGCGCT               [GA]GCGC^[CT]
             1613  AGCGCC              
MaeII         133  ACGT                 A^CGT
             2180  ACGT                
MluNI         611  TGGCCA               TGG^CCA
             1847  TGGCCA              
MscI          611  TGGCCA               TGG^CCA
             1847  TGGCCA              
MvnI          505  CGCG                 CG^CG
             1654  CGCG                
SmaI          925  CCCGGG               CCC^GGG
             1123  CCCGGG              
TaqI          686  TCGA                 T^CGA
             2095  TCGA                
TthHB8I       686  TCGA                 T^CGA
             2095  TCGA                
XcmI          599  CCAAGAAGAGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              843  CCAAGAGGCAGATGG     
XmaCI         925  CCCGGG               C^CCGGG
             1123  CCCGGG              
XmaI          925  CCCGGG               C^CCGGG
             1123  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AfaI        AhdI        AluI        
AlwI        AlwNI       ApoI        AspEI       AvaI        
AvaII       BanI        BanII       BbvCI       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
Eam1105I    EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI