##################################
# NO CUT ENZYMES (   46 enzymes) #
##################################

AccIII      AclI        AscI        AseI        AsnI        
BciVI       BseAI       BsiWI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        EcoRV       
EcoT22I     FseI        MluI        MroI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PmeI        PshBI       Psp1406I    PvuI        
RsrII       SalI        ScaI        SfuI        SnaBI       
SpeI        SspBI       SspI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII        5418  GACGTC               GACGT^C
Acc65I       6116  GGTACC               G^GTACC
AccI         1621  GTCTAC               GT^[AC][GT]AC
AflIII        378  ACGTGT               A^C[GA][CT]GT
AgeI         7472  ACCGGT               A^CCGGT
AhdI         6380  GACCTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      3270  AGCGCT               AGC^GCT
Asp700I      4215  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      6116  GGTACC               G^GTACC
AspEI        6380  GACCTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         3122  GGCGCC               GGCGC^C
BcgI         5089  CGACACTATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI        7208  GATCAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        6688  CGGCCG               CG[GA][CT]^CG
BsmI         2904  GCATTC               GAATGC(1/-1)
BspHI        6878  TCATGA               T^CATGA
BstEII       7043  GGTGACC              G^GT[ACGT]ACC
BstPI        7043  GGTGACC              G^GT[ACGT]ACC
DraI         7561  TTTAAA               TTT^AAA
DrdI         2247  GACCACAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         6688  CGGCCG               C^GGCCG
Eam1105I     6380  GACCTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        6688  CGGCCG               C^GGCCG
Eco47III     3270  AGCGCT               AGC^GCT
Eco52I       6688  CGGCCG               C^GGCCG
EcoO65I      7043  GGTGACC              G^GT[ACGT]ACC
EcoRI        3459  GAATTC               G^AATTC
HincII        613  GTTAAC               GT[CT]^[GA]AC
HindII        613  GTTAAC               GT[CT]^[GA]AC
HindIII       164  AAGCTT               A^AGCTT
HpaI          613  GTTAAC               GTT^AAC
KasI         3122  GGCGCC               G^GCGCC
KpnI         6116  GGTACC               GGTAC^C
KspI         6666  CCGCGG               CCGC^GG
MamI         7208  GATCAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         4678  CAATTG               C^AATTG
MunI         4678  CAATTG               C^AATTG
NarI         3122  GGCGCC               GG^CGCC
NdeI          700  CATATG               CA^TATG
PinAI        7472  ACCGGT               A^CCGGT
PshAI        1614  GACAGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         6878  TCATGA               T^CATGA
SacII        6666  CCGCGG               CCGC^GG
SapI         7407  GCTCTTC              GCTCTTC(1/4)
SexAI        5315  ACCAGGT              A^CC[AT]GGT
SfoI         3122  GGCGCC               GGC^GCC
SphI         1284  GCATGC               GCATG^C
XmnI         4215  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI         3459  GAATTC               [GA]^AATT[CT]
             7597  AAATTT              
AflII         134  CTTAAG               C^TTAAG
             5578  CTTAAG              
Alw44I        170  GTGCAC               G^TGCAC
             1463  GTGCAC              
ApaLI         170  GTGCAC               G^TGCAC
             1463  GTGCAC              
ApoI         3459  GAATTC               [GA]^AATT[CT]
             7597  AAATTT              
AviII        1943  TGCGCA               TGC^GCA
             6497  TGCGCA              
BamHI        6470  GGATCC               G^GATCC
             7146  GGATCC              
BclI           72  TGATCA               T^GATCA
             6614  TGATCA              
BfrI          134  CTTAAG               C^TTAAG
             5578  CTTAAG              
BglII         915  AGATCT               A^GATCT
             7320  AGATCT              
BsaWI        5797  TCCGGT               [AT]^CCGG[AT]
             7472  ACCGGT              
BsmBI         781  GAGACG               CGTCTC(1/5)
             4387  GAGACG              
BsrDI        1963  CATTGC               GCAATG(2/0)
             7109  CATTGC              
BssSI         631  CACGAG               CACGAG(-5/-1)
             4384  CACGAG              
FbaI           72  TGATCA               T^GATCA
             6614  TGATCA              
FspI         1943  TGCGCA               TGC^GCA
             6497  TGCGCA              
SbfI         5232  CCTGCAGG             CCTGCA^GG
             6993  CCTGCAGG            
SfiI         4609  GGCCAGGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             6964  GGCCTCCGGGGCC       
Sse8387I     5232  CCTGCAGG             CCTGCA^GG
             6993  CCTGCAGG            


#########################################
# 3 OR MORE CUT ENZYMES (  145 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AspI        AvaI        AvaII       AvrII       BalI        
BanI        BanII       BbrPI       BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflFI       PflMI       PleI        PmaCI       
PmlI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SgrAI       SmaI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI