################################## # NO CUT ENZYMES ( 46 enzymes) # ################################## AccIII AclI AscI AseI AsnI BciVI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EcoRV EcoT22I FseI MluI MroI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII SalI ScaI SfuI SnaBI SpeI SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 5418 GACGTC GACGT^C Acc65I 6116 GGTACC G^GTACC AccI 1621 GTCTAC GT^[AC][GT]AC AflIII 378 ACGTGT A^C[GA][CT]GT AgeI 7472 ACCGGT A^CCGGT AhdI 6380 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3270 AGCGCT AGC^GCT Asp700I 4215 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 6116 GGTACC G^GTACC AspEI 6380 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 3122 GGCGCC GGCGC^C BcgI 5089 CGACACTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 7208 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 6688 CGGCCG CG[GA][CT]^CG BsmI 2904 GCATTC GAATGC(1/-1) BspHI 6878 TCATGA T^CATGA BstEII 7043 GGTGACC G^GT[ACGT]ACC BstPI 7043 GGTGACC G^GT[ACGT]ACC DraI 7561 TTTAAA TTT^AAA DrdI 2247 GACCACAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 6688 CGGCCG C^GGCCG Eam1105I 6380 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 6688 CGGCCG C^GGCCG Eco47III 3270 AGCGCT AGC^GCT Eco52I 6688 CGGCCG C^GGCCG EcoO65I 7043 GGTGACC G^GT[ACGT]ACC EcoRI 3459 GAATTC G^AATTC HincII 613 GTTAAC GT[CT]^[GA]AC HindII 613 GTTAAC GT[CT]^[GA]AC HindIII 164 AAGCTT A^AGCTT HpaI 613 GTTAAC GTT^AAC KasI 3122 GGCGCC G^GCGCC KpnI 6116 GGTACC GGTAC^C KspI 6666 CCGCGG CCGC^GG MamI 7208 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4678 CAATTG C^AATTG MunI 4678 CAATTG C^AATTG NarI 3122 GGCGCC GG^CGCC NdeI 700 CATATG CA^TATG PinAI 7472 ACCGGT A^CCGGT PshAI 1614 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 6878 TCATGA T^CATGA SacII 6666 CCGCGG CCGC^GG SapI 7407 GCTCTTC GCTCTTC(1/4) SexAI 5315 ACCAGGT A^CC[AT]GGT SfoI 3122 GGCGCC GGC^GCC SphI 1284 GCATGC GCATG^C XmnI 4215 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 3459 GAATTC [GA]^AATT[CT] 7597 AAATTT AflII 134 CTTAAG C^TTAAG 5578 CTTAAG Alw44I 170 GTGCAC G^TGCAC 1463 GTGCAC ApaLI 170 GTGCAC G^TGCAC 1463 GTGCAC ApoI 3459 GAATTC [GA]^AATT[CT] 7597 AAATTT AviII 1943 TGCGCA TGC^GCA 6497 TGCGCA BamHI 6470 GGATCC G^GATCC 7146 GGATCC BclI 72 TGATCA T^GATCA 6614 TGATCA BfrI 134 CTTAAG C^TTAAG 5578 CTTAAG BglII 915 AGATCT A^GATCT 7320 AGATCT BsaWI 5797 TCCGGT [AT]^CCGG[AT] 7472 ACCGGT BsmBI 781 GAGACG CGTCTC(1/5) 4387 GAGACG BsrDI 1963 CATTGC GCAATG(2/0) 7109 CATTGC BssSI 631 CACGAG CACGAG(-5/-1) 4384 CACGAG FbaI 72 TGATCA T^GATCA 6614 TGATCA FspI 1943 TGCGCA TGC^GCA 6497 TGCGCA SbfI 5232 CCTGCAGG CCTGCA^GG 6993 CCTGCAGG SfiI 4609 GGCCAGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 6964 GGCCTCCGGGGCC Sse8387I 5232 CCTGCAGG CCTGCA^GG 6993 CCTGCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 145 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AspI AvaI AvaII AvrII BalI BanI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SgrAI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI