##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
Acc65I AscI AseI AsnI Asp718I
BsiEI BsmI BspDI BspLU11I BstBI
BstEII BstPI ClaI EagI EclXI
Eco52I EcoO65I EcoRV FseI KpnI
KspI MluI NdeI NotI NruI
NspV PacI PaeR7I PmeI PshBI
PvuI SacII SalI SbfI SexAI
SfiI SfuI SgrAI SpeI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1827 GACGTC GACGT^C
AflII 6733 CTTAAG C^TTAAG
AgeI 5884 ACCGGT A^CCGGT
AhdI 1087 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4379 AGCGCT AGC^GCT
AspEI 1087 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2774 TGCGCA TGC^GCA
AvrII 7554 CCTAGG C^CTAGG
BamHI 553 GGATCC G^GATCC
BciVI 7965 GTATCC GTATCC(6/5)
BfrI 6733 CTTAAG C^TTAAG
BglII 5460 AGATCT A^GATCT
BlnI 7554 CCTAGG C^CTAGG
BsaBI 650 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 7502 CGTACG C^GTACG
Bsp1407I 5664 TGTACA T^GTACA
BsrDI 7040 GCAATG GCAATG(2/0)
BsrGI 5664 TGTACA T^GTACA
BssHII 3093 GCGCGC G^CGCGC
BssSI 5787 CACGAG CACGAG(-5/-1)
Bst1107I 7254 GTATAC GTA^TAC
BstAPI 5101 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 7254 GTATAC GTA^TAC
DrdI 1821 GACATTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1087 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4379 AGCGCT AGC^GCT
EcoRI 2866 GAATTC G^AATTC
EcoT22I 4843 ATGCAT ATGCA^T
FspI 2774 TGCGCA TGC^GCA
HpaI 7631 GTTAAC GTT^AAC
MamI 650 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 536 CAATTG C^AATTG
MunI 536 CAATTG C^AATTG
NaeI 1931 GCCGGC GCC^GGC
NgoMIV 1931 GCCGGC G^CCGGC
NheI 6681 GCTAGC G^CTAGC
NsiI 4843 ATGCAT ATGCA^T
PinAI 5884 ACCGGT A^CCGGT
SacI 1897 GAGCTC GAGCT^C
ScaI 217 AGTACT AGT^ACT
SnaBI 7504 TACGTA TAC^GTA
SspBI 5664 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 4862 GTCTAC GT^[AC][GT]AC
7254 GTATAC
AccIII 2478 TCCGGA T^CCGGA
4145 TCCGGA
AclI 1215 AACGTT AA^CGTT
7812 AACGTT
Asp700I 2862 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7858 GAAATTTTTC
BbsI 990 GAAGAC GAAGAC(2/6)
1462 GAAGAC
BclI 7051 TGATCA T^GATCA
7919 TGATCA
BlpI 3534 GCTGAGC GC^T[ACGT]AGC
4764 GCTGAGC
Bpu1102I 3534 GCTGAGC GC^T[ACGT]AGC
4764 GCTGAGC
BpuAI 990 GAAGAC GAAGAC(2/6)
1462 GAAGAC
BseAI 2478 TCCGGA T^CCGGA
4145 TCCGGA
BsmBI 748 GAGACG CGTCTC(1/5)
1882 GAGACG
BspEI 2478 TCCGGA T^CCGGA
4145 TCCGGA
BspHI 7652 TCATGA T^CATGA
7745 TCATGA
BsrBI 5919 CCGCTC CCGCTC(-3/-3)
6739 GAGCGG
CelII 3534 GCTGAGC GC^T[ACGT]AGC
4764 GCTGAGC
CpoI 3457 CGGACCG CG^G[AT]CCG
7497 CGGTCCG
DraI 5641 TTTAAA TTT^AAA
8029 TTTAAA
DraIII 2722 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
6137 CACCATGTG
FbaI 7051 TGATCA T^GATCA
7919 TGATCA
MroI 2478 TCCGGA T^CCGGA
4145 TCCGGA
PshAI 1693 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1755 GACTCCGGTC
Psp1406I 1215 AACGTT AA^CGTT
7812 AACGTT
RcaI 7652 TCATGA T^CATGA
7745 TCATGA
RsrII 3457 CGGACCG CG^G[AT]CCG
7497 CGGTCCG
XbaI 51 TCTAGA T^CTAGA
5484 TCTAGA
XmnI 2862 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7858 GAAATTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
SphI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI