##################################
# NO CUT ENZYMES (   49 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        ApoI        
AscI        Asp700I     Asp718I     AvrII       BamHI       
BciVI       BglII       BlnI        BsaBI       BsiWI       
BspDI       BspHI       BssHII      Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DrdI        EcoRI       
EcoRV       EcoT22I     HpaI        KpnI        MamI        
MluI        NdeI        NotI        NruI        NsiI        
NspV        PacI        PmeI        Psp1406I    PvuI        
RcaI        RsrII       ScaI        SfuI        SgrAI       
SnaBI       SpeI        SspI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII       3948  TCCGGA               T^CCGGA
AflII        7001  CTTAAG               C^TTAAG
AgeI         6424  ACCGGT               A^CCGGT
AhdI         5120  GACTTTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        5120  GACTTTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         6122  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        3495  TGCGCA               TGC^GCA
BbeI         1941  GGCGCC               GGCGC^C
BbrPI        6519  CACGTG               CAC^GTG
BcgI         2808  CGAGGGGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         7272  TGATCA               T^GATCA
BfrI         7001  CTTAAG               C^TTAAG
BseAI        3948  TCCGGA               T^CCGGA
BspEI        3948  TCCGGA               T^CCGGA
BspLU11I     1560  ACATGT               A^CATGT
BstEII       3512  GGTTACC              G^GT[ACGT]ACC
BstPI        3512  GGTTACC              G^GT[ACGT]ACC
DraI         6827  TTTAAA               TTT^AAA
EagI         2448  CGGCCG               C^GGCCG
Eam1105I     5120  GACTTTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2448  CGGCCG               C^GGCCG
Eco52I       2448  CGGCCG               C^GGCCG
EcoO65I      3512  GGTTACC              G^GT[ACGT]ACC
FbaI         7272  TGATCA               T^GATCA
FseI         2449  GGCCGGCC             GGCCGG^CC
FspI         3495  TGCGCA               TGC^GCA
HindIII      3893  AAGCTT               A^AGCTT
KasI         1941  GGCGCC               G^GCGCC
MfeI         7520  CAATTG               C^AATTG
MroI         3948  TCCGGA               T^CCGGA
MunI         7520  CAATTG               C^AATTG
NaeI         2450  GCCGGC               GCC^GGC
NarI         1941  GGCGCC               GG^CGCC
NgoMIV       2450  GCCGGC               G^CCGGC
PaeR7I       3989  CTCGAG               C^TCGAG
PflFI        6122  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        6424  ACCGGT               A^CCGGT
PmaCI        6519  CACGTG               CAC^GTG
PmlI         6519  CACGTG               CAC^GTG
PshAI        7316  GACATGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI        5103  ACCAGGT              A^CC[AT]GGT
SfiI          911  GGCCCCGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1941  GGCGCC               GGC^GCC
SphI         3192  GCATGC               GCATG^C
SwaI         6826  ATTTAAAT             ATTT^AAAT
TfiI          344  GAATC                G^A[AT]TC
Tth111I      6122  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         3989  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI          788  GTCGAC               GT^[AC][GT]AC
             2543  GTCGAC              
AflIII       1485  ACACGT               A^C[GA][CT]GT
             1560  ACATGT              
Alw44I       2421  GTGCAC               G^TGCAC
             3311  GTGCAC              
ApaLI        2421  GTGCAC               G^TGCAC
             3311  GTGCAC              
AseI         1016  ATTAAT               AT^TAAT
             1826  ATTAAT              
AsnI         1016  ATTAAT               AT^TAAT
             1826  ATTAAT              
BsaAI        1486  CACGTA               [CT]AC^GT[GA]
             6519  CACGTG              
BsaI         3411  GAGACC               GGTCTC(1/5)
             6427  GGTCTC              
BsiEI        1393  CGACCG               CG[GA][CT]^CG
             2448  CGGCCG              
BsmI         3049  GAATGC               GAATGC(1/-1)
             5954  GCATTC              
Bsp1407I     1785  TGTACA               T^GTACA
             3841  TGTACA              
BsrGI        1785  TGTACA               T^GTACA
             3841  TGTACA              
BssSI        2651  CACGAG               CACGAG(-5/-1)
             6771  CTCGTG              
KspI         2587  CCGCGG               CCGC^GG
             3469  CCGCGG              
NheI         1472  GCTAGC               G^CTAGC
             5714  GCTAGC              
PshBI        1016  ATTAAT               AT^TAAT
             1826  ATTAAT              
SacII        2587  CCGCGG               CCGC^GG
             3469  CCGCGG              
SalI          788  GTCGAC               G^TCGAC
             2543  GTCGAC              
SmaI         2551  CCCGGG               CCC^GGG
             5490  CCCGGG              
SspBI        1785  TGTACA               T^GTACA
             3841  TGTACA              
XmaCI        2551  CCCGGG               C^CCGGG
             5490  CCCGGG              
XmaI         2551  CCCGGG               C^CCGGG
             5490  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  139 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        Aor51HI     ApaI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrDI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
Eco47III    Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
SbfI        ScrFI       SfaNI       SfcI        SmlI        
Sse8387I    StuI        StyI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XbaI        XcmI        XhoII