##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
AscI AseI AsnI Asp718I AspEI
AspI BciVI BclI BfrI BspDI
BssHII Bst1107I BstZ17I ClaI CpoI
DraI Eam1105I EcoRI FbaI HpaI
KpnI MfeI MluI MunI NdeI
NheI NotI PacI PflFI PmeI
PshBI Psp1406I RsrII ScaI SgrAI
SnaBI SpeI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 5544 TCCGGA T^CCGGA
AcsI 5275 AAATTC [GA]^AATT[CT]
AgeI 2092 ACCGGT A^CCGGT
ApoI 5275 AAATTC [GA]^AATT[CT]
AvrII 3030 CCTAGG C^CTAGG
BbrPI 294 CACGTG CAC^GTG
BglII 6020 AGATCT A^GATCT
BlnI 3030 CCTAGG C^CTAGG
BsaAI 294 CACGTG [CT]AC^GT[GA]
BsaBI 5064 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 5544 TCCGGA T^CCGGA
BsiWI 5471 CGTACG C^GTACG
BsmBI 1483 GAGACG CGTCTC(1/5)
BspEI 5544 TCCGGA T^CCGGA
BspLU11I 6951 ACATGT A^CATGT
BstBI 2459 TTCGAA TT^CGAA
EcoRV 5064 GATATC GAT^ATC
EcoT22I 788 ATGCAT ATGCA^T
FseI 3350 GGCCGGCC GGCCGG^CC
MamI 5064 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 5544 TCCGGA T^CCGGA
NruI 4533 TCGCGA TCG^CGA
NsiI 788 ATGCAT ATGCA^T
NspV 2459 TTCGAA TT^CGAA
PinAI 2092 ACCGGT A^CCGGT
PmaCI 294 CACGTG CAC^GTG
PmlI 294 CACGTG CAC^GTG
PshAI 3003 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1043 CGATCG CGAT^CG
SfuI 2459 TTCGAA TT^CGAA
SphI 401 GCATGC GCATG^C
SspI 6986 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 463 GTGCAC G^TGCAC
2970 GTGCAC
ApaLI 463 GTGCAC G^TGCAC
2970 GTGCAC
BbeI 4517 GGCGCC GGCGC^C
6053 GGCGCC
BsaWI 2092 ACCGGT [AT]^CCGG[AT]
5544 TCCGGA
Bsp1407I 314 TGTACA T^GTACA
663 TGTACA
BspHI 3722 TCATGA T^CATGA
4649 TCATGA
BsrGI 314 TGTACA T^GTACA
663 TGTACA
DrdI 2728 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2851 GACCGCATTGTC
HindIII 1195 AAGCTT A^AGCTT
6292 AAGCTT
KasI 4517 GGCGCC G^GCGCC
6053 GGCGCC
NarI 4517 GGCGCC GG^CGCC
6053 GGCGCC
RcaI 3722 TCATGA T^CATGA
4649 TCATGA
SalI 6205 GTCGAC G^TCGAC
7303 GTCGAC
SapI 7084 GAAGAGC GCTCTTC(1/4)
1176 GCTCTTC
SexAI 4961 ACCAGGT A^CC[AT]GGT
6404 ACCTGGT
SfiI 3375 GGCCCTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5379 GGCCACCGTGGCC
SfoI 4517 GGCGCC GGC^GCC
6053 GGCGCC
SspBI 314 TGTACA T^GTACA
663 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 163 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI ApaI Asp700I AspHI AvaI
AvaII AviII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PaeR7I PflMI
PleI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmaI SmlI
Sse8387I StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII
XmaCI XmaI XmnI