################################## # NO CUT ENZYMES ( 31 enzymes) # ################################## AscI AvrII BbeI BcgI BciVI BlnI BsiEI BsiWI CpoI EagI EclXI Eco52I FseI KasI KspI MluI NarI NdeI NheI NotI NruI PacI PmeI PvuI RsrII SacII SalI SfoI SgrAI SnaBI XbaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AclI 6670 AACGTT AA^CGTT AflII 4762 CTTAAG C^TTAAG AgeI 2129 ACCGGT A^CCGGT AhdI 374 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 4937 AGCGCT AGC^GCT AspEI 374 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1185 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 2623 CACGTG CAC^GTG BfrI 4762 CTTAAG C^TTAAG BsaAI 2623 CACGTG [CT]AC^GT[GA] BspDI 2815 ATCGAT AT^CGAT BsrDI 653 GCAATG GCAATG(2/0) BssHII 1213 GCGCGC G^CGCGC BssSI 3769 CACGAG CACGAG(-5/-1) Bst1107I 1081 GTATAC GTA^TAC BstEII 1158 GGTCACC G^GT[ACGT]ACC BstPI 1158 GGTCACC G^GT[ACGT]ACC BstZ17I 1081 GTATAC GTA^TAC ClaI 2815 ATCGAT AT^CGAT DrdI 3442 GACATGACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 374 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4937 AGCGCT AGC^GCT EcoO65I 1158 GGTCACC G^GT[ACGT]ACC HpaI 93 GTTAAC GTT^AAC PflFI 1185 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2129 ACCGGT A^CCGGT PmaCI 2623 CACGTG CAC^GTG PmlI 2623 CACGTG CAC^GTG PshAI 1173 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 6670 AACGTT AA^CGTT SbfI 5052 CCTGCAGG CCTGCA^GG SfiI 2496 GGCCCGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 1773 ACTAGT A^CTAGT Sse8387I 5052 CCTGCAGG CCTGCA^GG SwaI 3983 ATTTAAAT ATTT^AAAT Tth111I 1185 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 707 GGTACC G^GTACC 3215 GGTACC AccI 1081 GTATAC GT^[AC][GT]AC 8814 GTAGAC AflIII 4535 ACATGT A^C[GA][CT]GT 5465 ACATGT ApaI 2495 GGGCCC GGGCC^C 2996 GGGCCC Asp718I 707 GGTACC G^GTACC 3215 GGTACC AviII 2978 TGCGCA TGC^GCA 3785 TGCGCA BamHI 56 GGATCC G^GATCC 7303 GGATCC BclI 1205 TGATCA T^GATCA 5081 TGATCA BsaBI 1743 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2073 GATTCGAATC BsaI 1171 GAGACC GGTCTC(1/5) 1948 GAGACC BsmBI 2767 CGTCTC CGTCTC(1/5) 2068 GAGACG Bsp1407I 174 TGTACA T^GTACA 8233 TGTACA BspLU11I 4535 ACATGT A^CATGT 5465 ACATGT BsrGI 174 TGTACA T^GTACA 8233 TGTACA BstAPI 1091 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4581 GCACTCCCTGC BstBI 1842 TTCGAA TT^CGAA 2075 TTCGAA DraIII 7595 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG 8013 CACCAGGTG EcoRV 211 GATATC GAT^ATC 8105 GATATC EcoT22I 5568 ATGCAT ATGCA^T 8277 ATGCAT FbaI 1205 TGATCA T^GATCA 5081 TGATCA FspI 2978 TGCGCA TGC^GCA 3785 TGCGCA KpnI 707 GGTACC GGTAC^C 3215 GGTACC MamI 1743 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2073 GATTCGAATC MfeI 167 CAATTG C^AATTG 6824 CAATTG MunI 167 CAATTG C^AATTG 6824 CAATTG NaeI 3847 GCCGGC GCC^GGC 7216 GCCGGC NgoMIV 3847 GCCGGC G^CCGGC 7216 GCCGGC NsiI 5568 ATGCAT ATGCA^T 8277 ATGCAT NspV 1842 TTCGAA TT^CGAA 2075 TTCGAA PaeR7I 3778 CTCGAG C^TCGAG 4111 CTCGAG SapI 1735 GAAGAGC GCTCTTC(1/4) 5070 GCTCTTC ScaI 6178 AGTACT AGT^ACT 8420 AGTACT SexAI 4466 ACCTGGT A^CC[AT]GGT 8014 ACCAGGT SfuI 1842 TTCGAA TT^CGAA 2075 TTCGAA SmaI 8 CCCGGG CCC^GGG 2552 CCCGGG SspBI 174 TGTACA T^GTACA 8233 TGTACA XhoI 3778 CTCGAG C^TCGAG 4111 CTCGAG XmaCI 8 CCCGGG C^CCGGG 2552 CCCGGG XmaI 8 CCCGGG C^CCGGG 2552 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 152 enzymes) # ######################################### AatII AccII AccIII AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshBI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI