##################################
# NO CUT ENZYMES (   31 enzymes) #
##################################

AscI        AvrII       BbeI        BcgI        BciVI       
BlnI        BsiEI       BsiWI       CpoI        EagI        
EclXI       Eco52I      FseI        KasI        KspI        
MluI        NarI        NdeI        NheI        NotI        
NruI        PacI        PmeI        PvuI        RsrII       
SacII       SalI        SfoI        SgrAI       SnaBI       
XbaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AclI         6670  AACGTT               AA^CGTT
AflII        4762  CTTAAG               C^TTAAG
AgeI         2129  ACCGGT               A^CCGGT
AhdI          374  GACAAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      4937  AGCGCT               AGC^GCT
AspEI         374  GACAAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1185  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        2623  CACGTG               CAC^GTG
BfrI         4762  CTTAAG               C^TTAAG
BsaAI        2623  CACGTG               [CT]AC^GT[GA]
BspDI        2815  ATCGAT               AT^CGAT
BsrDI         653  GCAATG               GCAATG(2/0)
BssHII       1213  GCGCGC               G^CGCGC
BssSI        3769  CACGAG               CACGAG(-5/-1)
Bst1107I     1081  GTATAC               GTA^TAC
BstEII       1158  GGTCACC              G^GT[ACGT]ACC
BstPI        1158  GGTCACC              G^GT[ACGT]ACC
BstZ17I      1081  GTATAC               GTA^TAC
ClaI         2815  ATCGAT               AT^CGAT
DrdI         3442  GACATGACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      374  GACAAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     4937  AGCGCT               AGC^GCT
EcoO65I      1158  GGTCACC              G^GT[ACGT]ACC
HpaI           93  GTTAAC               GTT^AAC
PflFI        1185  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        2129  ACCGGT               A^CCGGT
PmaCI        2623  CACGTG               CAC^GTG
PmlI         2623  CACGTG               CAC^GTG
PshAI        1173  GACCTACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     6670  AACGTT               AA^CGTT
SbfI         5052  CCTGCAGG             CCTGCA^GG
SfiI         2496  GGCCCGCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         1773  ACTAGT               A^CTAGT
Sse8387I     5052  CCTGCAGG             CCTGCA^GG
SwaI         3983  ATTTAAAT             ATTT^AAAT
Tth111I      1185  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

Acc65I        707  GGTACC               G^GTACC
             3215  GGTACC              
AccI         1081  GTATAC               GT^[AC][GT]AC
             8814  GTAGAC              
AflIII       4535  ACATGT               A^C[GA][CT]GT
             5465  ACATGT              
ApaI         2495  GGGCCC               GGGCC^C
             2996  GGGCCC              
Asp718I       707  GGTACC               G^GTACC
             3215  GGTACC              
AviII        2978  TGCGCA               TGC^GCA
             3785  TGCGCA              
BamHI          56  GGATCC               G^GATCC
             7303  GGATCC              
BclI         1205  TGATCA               T^GATCA
             5081  TGATCA              
BsaBI        1743  GATCAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2073  GATTCGAATC          
BsaI         1171  GAGACC               GGTCTC(1/5)
             1948  GAGACC              
BsmBI        2767  CGTCTC               CGTCTC(1/5)
             2068  GAGACG              
Bsp1407I      174  TGTACA               T^GTACA
             8233  TGTACA              
BspLU11I     4535  ACATGT               A^CATGT
             5465  ACATGT              
BsrGI         174  TGTACA               T^GTACA
             8233  TGTACA              
BstAPI       1091  GCAACTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4581  GCACTCCCTGC         
BstBI        1842  TTCGAA               TT^CGAA
             2075  TTCGAA              
DraIII       7595  CACTAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             8013  CACCAGGTG           
EcoRV         211  GATATC               GAT^ATC
             8105  GATATC              
EcoT22I      5568  ATGCAT               ATGCA^T
             8277  ATGCAT              
FbaI         1205  TGATCA               T^GATCA
             5081  TGATCA              
FspI         2978  TGCGCA               TGC^GCA
             3785  TGCGCA              
KpnI          707  GGTACC               GGTAC^C
             3215  GGTACC              
MamI         1743  GATCAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2073  GATTCGAATC          
MfeI          167  CAATTG               C^AATTG
             6824  CAATTG              
MunI          167  CAATTG               C^AATTG
             6824  CAATTG              
NaeI         3847  GCCGGC               GCC^GGC
             7216  GCCGGC              
NgoMIV       3847  GCCGGC               G^CCGGC
             7216  GCCGGC              
NsiI         5568  ATGCAT               ATGCA^T
             8277  ATGCAT              
NspV         1842  TTCGAA               TT^CGAA
             2075  TTCGAA              
PaeR7I       3778  CTCGAG               C^TCGAG
             4111  CTCGAG              
SapI         1735  GAAGAGC              GCTCTTC(1/4)
             5070  GCTCTTC             
ScaI         6178  AGTACT               AGT^ACT
             8420  AGTACT              
SexAI        4466  ACCTGGT              A^CC[AT]GGT
             8014  ACCAGGT             
SfuI         1842  TTCGAA               TT^CGAA
             2075  TTCGAA              
SmaI            8  CCCGGG               CCC^GGG
             2552  CCCGGG              
SspBI         174  TGTACA               T^GTACA
             8233  TGTACA              
XhoI         3778  CTCGAG               C^TCGAG
             4111  CTCGAG              
XmaCI           8  CCCGGG               C^CCGGG
             2552  CCCGGG              
XmaI            8  CCCGGG               C^CCGGG
             2552  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  152 enzymes) #
#########################################

AatII       AccII       AccIII      AciI        AcsI        
AcyI        AfaI        AluI        Alw44I      AlwI        
AlwNI       AocI        ApaLI       ApoI        AseI        
AsnI        Asp700I     AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspEI       
BspHI       BspMI       BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MroI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PshBI       
PstI        PvuII       RcaI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SphI        SspI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmnI