##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BcgI BciVI BclI
BglII BlnI BlpI Bpu1102I BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HindIII KspI MfeI MluI
MroI MunI MvnI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 340 GGTACC G^GTACC
AflII 2551 CTTAAG C^TTAAG
AocI 333 CCTCAGG CC^T[ACGT]AGG
ApaI 1696 GGGCCC GGGCC^C
Asp700I 2272 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 340 GGTACC G^GTACC
BamHI 1447 GGATCC G^GATCC
BbeI 432 GGCGCC GGCGC^C
BbrPI 291 CACGTG CAC^GTG
BbsI 1757 GAAGAC GAAGAC(2/6)
BfrI 2551 CTTAAG C^TTAAG
BpuAI 1757 GAAGAC GAAGAC(2/6)
BsaAI 291 CACGTG [CT]AC^GT[GA]
BsaBI 416 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 2909 TCATGA T^CATGA
BsrDI 956 CATTGC GCAATG(2/0)
BstAPI 1869 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 333 CCTCAGG CC^T[ACGT]AGG
DraIII 276 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1171 GACGCCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 333 CCTCAGG CC^T[ACGT]AGG
EcoRI 2651 GAATTC G^AATTC
HpaI 2957 GTTAAC GTT^AAC
KasI 432 GGCGCC G^GCGCC
KpnI 340 GGTACC GGTAC^C
MamI 416 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1205 GCCGGC GCC^GGC
NarI 432 GGCGCC GG^CGCC
NgoMIV 1205 GCCGGC G^CCGGC
PaeR7I 7 CTCGAG C^TCGAG
PmaCI 291 CACGTG CAC^GTG
PmlI 291 CACGTG CAC^GTG
PpuMI 2784 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 2909 TCATGA T^CATGA
SacI 2096 GAGCTC GAGCT^C
SapI 689 GCTCTTC GCTCTTC(1/4)
SexAI 478 ACCTGGT A^CC[AT]GGT
SfoI 432 GGCGCC GGC^GCC
TfiI 1437 GATTC G^A[AT]TC
XhoI 7 CTCGAG C^TCGAG
XmnI 2272 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 2651 GAATTC [GA]^AATT[CT]
2691 GAATTT
AflIII 700 ACATGT A^C[GA][CT]GT
1623 ACATGT
AlwNI 874 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
1548 CAGGCACTG
ApoI 2651 GAATTC [GA]^AATT[CT]
2691 GAATTT
BbvCI 1000 CCTCAGC CCTCAGC(-5/-2)
807 GCTGAGG
BglI 1717 GCCACGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2207 GCCGCCTGGGC
Bsp1407I 703 TGTACA T^GTACA
2776 TGTACA
BspLU11I 700 ACATGT A^CATGT
1623 ACATGT
BspMI 682 ACCTGC ACCTGC(4/8)
1992 GCAGGT
BsrGI 703 TGTACA T^GTACA
2776 TGTACA
BstXI 1054 CCAGCTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1676 CCACTTTCCTGG
BstYI 1282 GGATCT [GA]^GATC[CT]
1447 GGATCC
DraII 1184 AGGCCCC [GA]G^G[ACGT]CC[CT]
2784 GGGTCCT
EcoO109I 1184 AGGCCCC [GA]G^G[ACGT]CC[CT]
2784 GGGTCCT
HaeII 432 GGCGCC [GA]GCGC^[CT]
1658 GGCGCT
MflI 1282 GGATCT [GA]^GATC[CT]
1447 GGATCC
NcoI 355 CCATGG C^CATGG
1132 CCATGG
PleI 38 GACTC GAGTC(4/5)
876 GACTC
PstI 684 CTGCAG CTGCA^G
1294 CTGCAG
PvuII 363 CAGCTG CAG^CTG
1701 CAGCTG
SmlI 7 CTCGAG C^T[CT][GA]AG
2551 CTTAAG
SspBI 703 TGTACA T^GTACA
2776 TGTACA
XhoII 1282 GGATCT [GA]^GATC[CT]
1447 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoNI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI