##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AviII       AvrII       BcgI        BciVI       BclI        
BglII       BlnI        BlpI        Bpu1102I    BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BspDI       BspEI       BsrBI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     CelII       ClaI        CpoI        DraI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     KspI        MfeI        MluI        
MroI        MunI        MvnI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tth111I     XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        340  GGTACC               G^GTACC
AflII        2551  CTTAAG               C^TTAAG
AocI          333  CCTCAGG              CC^T[ACGT]AGG
ApaI         1696  GGGCCC               GGGCC^C
Asp700I      2272  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       340  GGTACC               G^GTACC
BamHI        1447  GGATCC               G^GATCC
BbeI          432  GGCGCC               GGCGC^C
BbrPI         291  CACGTG               CAC^GTG
BbsI         1757  GAAGAC               GAAGAC(2/6)
BfrI         2551  CTTAAG               C^TTAAG
BpuAI        1757  GAAGAC               GAAGAC(2/6)
BsaAI         291  CACGTG               [CT]AC^GT[GA]
BsaBI         416  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI        2909  TCATGA               T^CATGA
BsrDI         956  CATTGC               GCAATG(2/0)
BstAPI       1869  GCAGAAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        333  CCTCAGG              CC^T[ACGT]AGG
DraIII        276  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1171  GACGCCCACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        333  CCTCAGG              CC^T[ACGT]AGG
EcoRI        2651  GAATTC               G^AATTC
HpaI         2957  GTTAAC               GTT^AAC
KasI          432  GGCGCC               G^GCGCC
KpnI          340  GGTACC               GGTAC^C
MamI          416  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         1205  GCCGGC               GCC^GGC
NarI          432  GGCGCC               GG^CGCC
NgoMIV       1205  GCCGGC               G^CCGGC
PaeR7I          7  CTCGAG               C^TCGAG
PmaCI         291  CACGTG               CAC^GTG
PmlI          291  CACGTG               CAC^GTG
PpuMI        2784  GGGTCCT              [GA]G^G[AT]CC[CT]
RcaI         2909  TCATGA               T^CATGA
SacI         2096  GAGCTC               GAGCT^C
SapI          689  GCTCTTC              GCTCTTC(1/4)
SexAI         478  ACCTGGT              A^CC[AT]GGT
SfoI          432  GGCGCC               GGC^GCC
TfiI         1437  GATTC                G^A[AT]TC
XhoI            7  CTCGAG               C^TCGAG
XmnI         2272  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI         2651  GAATTC               [GA]^AATT[CT]
             2691  GAATTT              
AflIII        700  ACATGT               A^C[GA][CT]GT
             1623  ACATGT              
AlwNI         874  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1548  CAGGCACTG           
ApoI         2651  GAATTC               [GA]^AATT[CT]
             2691  GAATTT              
BbvCI        1000  CCTCAGC              CCTCAGC(-5/-2)
              807  GCTGAGG             
BglI         1717  GCCACGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2207  GCCGCCTGGGC         
Bsp1407I      703  TGTACA               T^GTACA
             2776  TGTACA              
BspLU11I      700  ACATGT               A^CATGT
             1623  ACATGT              
BspMI         682  ACCTGC               ACCTGC(4/8)
             1992  GCAGGT              
BsrGI         703  TGTACA               T^GTACA
             2776  TGTACA              
BstXI        1054  CCAGCTCGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1676  CCACTTTCCTGG        
BstYI        1282  GGATCT               [GA]^GATC[CT]
             1447  GGATCC              
DraII        1184  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             2784  GGGTCCT             
EcoO109I     1184  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             2784  GGGTCCT             
HaeII         432  GGCGCC               [GA]GCGC^[CT]
             1658  GGCGCT              
MflI         1282  GGATCT               [GA]^GATC[CT]
             1447  GGATCC              
NcoI          355  CCATGG               C^CATGG
             1132  CCATGG              
PleI           38  GACTC                GAGTC(4/5)
              876  GACTC               
PstI          684  CTGCAG               CTGCA^G
             1294  CTGCAG              
PvuII         363  CAGCTG               CAG^CTG
             1701  CAGCTG              
SmlI            7  CTCGAG               C^T[CT][GA]AG
             2551  CTTAAG              
SspBI         703  TGTACA               T^GTACA
             2776  TGTACA              
XhoII        1282  GGATCT               [GA]^GATC[CT]
             1447  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrFI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        EcoNI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StuI        StyI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI