##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BciVI       BclI        
BglI        BlnI        BsaWI       BseAI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BsrBI       
BsrFI       BssHII      Cfr10I      ClaI        CpoI        
DrdI        Eco47III    FbaI        FseI        FspI        
HaeII       KasI        KpnI        KspI        MluI        
MroI        NaeI        NarI        NgoMIV      NotI        
NruI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StuI        Tth111I     
Van91I      XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII          36  GACGTC               GACGT^C
AccII          33  CGCG                 CG^CG
AcyI           36  GACGTC               G[GA]^CG[CT]C
AhdI           36  GACGTCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI          36  GACGTCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         4175  TGGCCA               TGG^CCA
BanII        4222  GAGCTC               G[GA]GC[CT]^C
BcgI         2544  CGACACCATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         940  AGATCT               A^GATCT
BlpI         5153  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     5153  GCTAAGC              GC^T[ACGT]AGC
BsaAI        4389  TACGTG               [CT]AC^GT[GA]
BsaHI          36  GACGTC               G[GA]^CG[CT]C
BsiEI           6  CGGCCG               CG[GA][CT]^CG
BsmI          151  GCATTC               GAATGC(1/-1)
Bsp1407I      341  TGTACA               T^GTACA
BspHI        3377  TCATGA               T^CATGA
BsrGI         341  TGTACA               T^GTACA
BssSI        3450  CTCGTG               CACGAG(-5/-1)
BstAPI       3855  GCATATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        3943  TTCGAA               TT^CGAA
BstUI          33  CGCG                 CG^CG
BstXI        1046  CCAGCCATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        5153  GCTAAGC              GC^T[ACGT]AGC
DraIII       1426  CACGGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI            6  CGGCCG               C^GGCCG
Eam1105I       36  GACGTCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI           6  CGGCCG               C^GGCCG
Eco52I          6  CGGCCG               C^GGCCG
EcoRI        3772  GAATTC               G^AATTC
FauI         5529  GCGGG                CCCGC(4/6)
HgaI         1386  GCGTC                GACGC(5/10)
Hin1I          36  GACGTC               G[GA]^CG[CT]C
HpaI         3204  GTTAAC               GTT^AAC
MluNI        4175  TGGCCA               TGG^CCA
MscI         4175  TGGCCA               TGG^CCA
MvnI           33  CGCG                 CG^CG
NheI         4319  GCTAGC               G^CTAGC
NspV         3943  TTCGAA               TT^CGAA
PshAI        2940  GACTTTAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         3377  TCATGA               T^CATGA
SacI         4222  GAGCTC               GAGCT^C
SapI           17  GCTCTTC              GCTCTTC(1/4)
SexAI        2816  ACCAGGT              A^CC[AT]GGT
SfuI         3943  TTCGAA               TT^CGAA
SspBI         341  TGTACA               T^GTACA
XbaI         2615  TCTAGA               T^CTAGA
XcmI         6297  CCAAAGACAATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AflII        1299  CTTAAG               C^TTAAG
             2709  CTTAAG              
AocI         2751  CCTCAGG              CC^T[ACGT]AGG
             3579  CCTAAGG             
Asp700I      3408  GAAGGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5584  GAACATATTC          
AspHI        4222  GAGCTC               G[AT]GC[AT]^C
             4774  GTGCTC              
BcnI            4  CCCGG                CC^[GC]GG
                9  CCGGG               
BfrI         1299  CTTAAG               C^TTAAG
             2709  CTTAAG              
BsaBI        4113  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             5423  GATTATAATC          
BsiHKAI      4222  GAGCTC               G[AT]GC[AT]^C
             4774  GTGCTC              
BspMI        1155  ACCTGC               ACCTGC(4/8)
              240  GCAGGT              
BstEII       2113  GGTTACC              G^GT[ACGT]ACC
             3121  GGTTACC             
BstPI        2113  GGTTACC              G^GT[ACGT]ACC
             3121  GGTTACC             
Bsu36I       2751  CCTCAGG              CC^T[ACGT]AGG
             3579  CCTAAGG             
DraII        2236  AGGACCT              [GA]G^G[ACGT]CC[CT]
             4800  GGGACCT             
EaeI            6  CGGCCG               [CT]^GGCC[GA]
             4175  TGGCCA              
Eco81I       2751  CCTCAGG              CC^T[ACGT]AGG
             3579  CCTAAGG             
EcoNI        5231  CCTTATGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5769  CCTGAAACAGG         
EcoO109I     2236  AGGACCT              [GA]G^G[ACGT]CC[CT]
             4800  GGGACCT             
EcoO65I      2113  GGTTACC              G^GT[ACGT]ACC
             3121  GGTTACC             
EcoRV         184  GATATC               GAT^ATC
             4395  GATATC              
EcoT14I      4612  CCATGG               C^C[AT][AT]GG
             4793  CCATGG              
EcoT22I       349  ATGCAT               ATGCA^T
             5649  ATGCAT              
HapII           5  CCGG                 C^CGG
                9  CCGG                
HincII       3204  GTTAAC               GT[CT]^[GA]AC
             3936  GTTGAC              
HindII       3204  GTTAAC               GT[CT]^[GA]AC
             3936  GTTGAC              
HindIII      3786  AAGCTT               A^AGCTT
             4985  AAGCTT              
HpaII           5  CCGG                 C^CGG
                9  CCGG                
MaeII          37  ACGT                 A^CGT
             4390  ACGT                
MamI         4113  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             5423  GATTATAATC          
MspI            5  CCGG                 C^CGG
                9  CCGG                
NciI            4  CCCGG                CC^[GC]GG
                9  CCGGG               
NcoI         4612  CCATGG               C^CATGG
             4793  CCATGG              
NsiI          349  ATGCAT               ATGCA^T
             5649  ATGCAT              
PpuMI        2236  AGGACCT              [GA]G^G[AT]CC[CT]
             4800  GGGACCT             
PstI          238  CTGCAG               CTGCA^G
             1157  CTGCAG              
StyI         4612  CCATGG               C^C[AT][AT]GG
             4793  CCATGG              
XmnI         3408  GAAGGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5584  GAACATATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AseI        
AsnI        AvaII       BbsI        BbvCI       BbvI        
BfaI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspLU11I    BsrDI       
BsrI        BssKI       Bst1107I    BstF5I      BstNI       
BstYI       BstZ17I     Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DsaI        EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MfeI        
MflI        MnlI        MseI        MslI        MspA1I      
MunI        MvaI        MwoI        NdeI        NdeII       
NlaIII      NlaIV       NspI        PleI        PshBI       
PvuII       RsaI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        SspI        
SwaI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII