##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
AviII AvrII BbrPI BcgI BciVI
BfrI BglII BlnI BsaAI BsaBI
BseAI BsiEI BspDI BspEI BssHII
Bst1107I BstZ17I ClaI DraI EagI
EclXI Eco52I EcoRI EcoRV EcoT22I
FspI HincII HindII HindIII HpaI
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI SacII SalI SbfI ScaI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2583 GGTACC G^GTACC
AcsI 3333 AAATTC [GA]^AATT[CT]
AflIII 4890 ACATGT A^C[GA][CT]GT
AhdI 1722 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3601 AGCGCT AGC^GCT
ApoI 3333 AAATTC [GA]^AATT[CT]
Asp700I 143 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2583 GGTACC G^GTACC
AspEI 1722 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 41 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 4896 TGATCA T^GATCA
BsaWI 211 ACCGGA [AT]^CCGG[AT]
BsiWI 2623 CGTACG C^GTACG
Bsp1407I 4583 TGTACA T^GTACA
BspHI 151 TCATGA T^CATGA
BspLU11I 4890 ACATGT A^CATGT
BsrFI 2343 GCCGGC [GA]^CCGG[CT]
BsrGI 4583 TGTACA T^GTACA
BstBI 3594 TTCGAA TT^CGAA
Cfr10I 2343 GCCGGC [GA]^CCGG[CT]
CpoI 213 CGGACCG CG^G[AT]CCG
DraIII 1758 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1722 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3601 AGCGCT AGC^GCT
FbaI 4896 TGATCA T^GATCA
FseI 2342 GGCCGGCC GGCCGG^CC
KpnI 2583 GGTACC GGTAC^C
NaeI 2343 GCCGGC GCC^GGC
NgoMIV 2343 GCCGGC G^CCGGC
NspV 3594 TTCGAA TT^CGAA
PflFI 41 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 151 TCATGA T^CATGA
RsrII 213 CGGACCG CG^G[AT]CCG
SapI 3597 GAAGAGC GCTCTTC(1/4)
SexAI 1967 ACCAGGT A^CC[AT]GGT
SfuI 3594 TTCGAA TT^CGAA
SspBI 4583 TGTACA T^GTACA
SspI 4907 AATATT AAT^ATT
Tsp509I 3334 AATT ^AATT
Tth111I 41 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 143 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 633 GTAGAC GT^[AC][GT]AC
2886 GTCTAC
AocI 3630 CCTGAGG CC^T[ACGT]AGG
4395 CCTCAGG
BamHI 1085 GGATCC G^GATCC
1310 GGATCC
BbeI 1796 GGCGCC GGCGC^C
4689 GGCGCC
BbsI 3548 GAAGAC GAAGAC(2/6)
3775 GAAGAC
BlpI 574 GCTCAGC GC^T[ACGT]AGC
2269 GCTCAGC
Bpu1102I 574 GCTCAGC GC^T[ACGT]AGC
2269 GCTCAGC
BpuAI 3548 GAAGAC GAAGAC(2/6)
3775 GAAGAC
BsmI 143 GAATGC GAATGC(1/-1)
3088 GCATTC
BssSI 380 CACGAG CACGAG(-5/-1)
2633 CACGAG
BstAPI 2567 GCACGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2690 GCAGCCCCTGC
BstEII 941 GGTGACC G^GT[ACGT]ACC
1544 GGTCACC
BstPI 941 GGTGACC G^GT[ACGT]ACC
1544 GGTCACC
Bsu36I 3630 CCTGAGG CC^T[ACGT]AGG
4395 CCTCAGG
CelII 574 GCTCAGC GC^T[ACGT]AGC
2269 GCTCAGC
DrdI 3354 GACTGGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3567 GACAACAGGGTC
EarI 3597 GAAGAG CTCTTC(1/4)
3707 GAAGAG
Eco81I 3630 CCTGAGG CC^T[ACGT]AGG
4395 CCTCAGG
EcoNI 59 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1976 CCTGGGCAAGG
EcoO65I 941 GGTGACC G^GT[ACGT]ACC
1544 GGTCACC
KasI 1796 GGCGCC G^GCGCC
4689 GGCGCC
Ksp632I 3597 GAAGAG CTCTTC(1/4)
3707 GAAGAG
MaeII 35 ACGT A^CGT
3874 ACGT
MseI 4946 TTAA T^TAA
4956 TTAA
NarI 1796 GGCGCC GG^CGCC
4689 GGCGCC
SfiI 1496 GGCCTATGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3026 GGCCCCCAAGGCC
SfoI 1796 GGCGCC GGC^GCC
4689 GGCGCC
SmlI 924 CTCAAG C^T[CT][GA]AG
3198 CTTGAG
StuI 4482 AGGCCT AGG^CCT
4670 AGGCCT
TaqI 3595 TCGA T^CGA
4514 TCGA
Tru9I 4946 TTAA T^TAA
4956 TTAA
TthHB8I 3595 TCGA T^CGA
4514 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TfiI TseI
Tsp45I TspRI Van91I XcmI XhoII
XmaCI XmaI