##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
AseI AsnI AviII BbeI BclI
BsiEI BsiWI BssHII Bst1107I BstBI
BstZ17I CpoI DrdI EagI EclXI
Eco47III Eco52I EcoRV FbaI FseI
FspI HaeII HindIII HpaI KasI
MluI NarI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 3733 GGTACC G^GTACC
AccI 4647 GTCTAC GT^[AC][GT]AC
AcyI 3276 GACGCC G[GA]^CG[CT]C
ApaI 2382 GGGCCC GGGCC^C
Asp718I 3733 GGTACC G^GTACC
AvaI 5291 CCCGGG C^[CT]CG[GA]G
AvrII 4795 CCTAGG C^CTAGG
BbrPI 4926 CACGTG CAC^GTG
BbvCI 2708 CCTCAGC CCTCAGC(-5/-2)
BcgI 2140 CGAAGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1590 AGATCT A^GATCT
BlnI 4795 CCTAGG C^CTAGG
BsaAI 4926 CACGTG [CT]AC^GT[GA]
BsaHI 3276 GACGCC G[GA]^CG[CT]C
BsoBI 5291 CCCGGG C^[CT]CG[GA]G
BspDI 1233 ATCGAT AT^CGAT
BsrBI 2035 GAGCGG CCGCTC(-3/-3)
BsrDI 4054 CATTGC GCAATG(2/0)
BssSI 962 CTCGTG CACGAG(-5/-1)
ClaI 1233 ATCGAT AT^CGAT
EcoRI 1145 GAATTC G^AATTC
Hin1I 3276 GACGCC G[GA]^CG[CT]C
KpnI 3733 GGTACC GGTAC^C
KspI 2357 CCGCGG CCGC^GG
NaeI 5296 GCCGGC GCC^GGC
NgoMIV 5296 GCCGGC G^CCGGC
PmaCI 4926 CACGTG CAC^GTG
PmlI 4926 CACGTG CAC^GTG
SacII 2357 CCGCGG CCGC^GG
SapI 1644 GCTCTTC GCTCTTC(1/4)
SbfI 3209 CCTGCAGG CCTGCA^GG
SmaI 5291 CCCGGG CCC^GGG
SpeI 3620 ACTAGT A^CTAGT
Sse8387I 3209 CCTGCAGG CCTGCA^GG
SspI 954 AATATT AAT^ATT
XmaCI 5291 CCCGGG C^CCGGG
XmaI 5291 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 173 TCCGGA T^CCGGA
4584 TCCGGA
AflII 2462 CTTAAG C^TTAAG
5995 CTTAAG
AflIII 1707 ACATGT A^C[GA][CT]GT
5606 ACATGT
AhdI 1753 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2779 GACTTGCTGTC
AocI 1157 CCTGAGG CC^T[ACGT]AGG
5237 CCTTAGG
AspEI 1753 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2779 GACTTGCTGTC
BamHI 1081 GGATCC G^GATCC
4630 GGATCC
BciVI 881 GGATAC GTATCC(6/5)
4581 GTATCC
BfrI 2462 CTTAAG C^TTAAG
5995 CTTAAG
BlpI 1563 GCTCAGC GC^T[ACGT]AGC
2890 GCTCAGC
Bpu1102I 1563 GCTCAGC GC^T[ACGT]AGC
2890 GCTCAGC
BsaBI 1261 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1395 GATTGTCATC
BseAI 173 TCCGGA T^CCGGA
4584 TCCGGA
BspEI 173 TCCGGA T^CCGGA
4584 TCCGGA
BspHI 1450 TCATGA T^CATGA
5512 TCATGA
BspLU11I 1707 ACATGT A^CATGT
5606 ACATGT
BstEII 985 GGTGACC G^GT[ACGT]ACC
2969 GGTCACC
BstPI 985 GGTGACC G^GT[ACGT]ACC
2969 GGTCACC
Bsu36I 1157 CCTGAGG CC^T[ACGT]AGG
5237 CCTTAGG
CelII 1563 GCTCAGC GC^T[ACGT]AGC
2890 GCTCAGC
DraI 6189 TTTAAA TTT^AAA
6379 TTTAAA
DraIII 3377 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
6008 CACACAGTG
Eam1105I 1753 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2779 GACTTGCTGTC
Eco81I 1157 CCTGAGG CC^T[ACGT]AGG
5237 CCTTAGG
EcoO65I 985 GGTGACC G^GT[ACGT]ACC
2969 GGTCACC
HincII 5591 GTCAAC GT[CT]^[GA]AC
6077 GTCAAC
HindII 5591 GTCAAC GT[CT]^[GA]AC
6077 GTCAAC
MamI 1261 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1395 GATTGTCATC
MfeI 969 CAATTG C^AATTG
3116 CAATTG
MroI 173 TCCGGA T^CCGGA
4584 TCCGGA
MunI 969 CAATTG C^AATTG
3116 CAATTG
NdeI 2752 CATATG CA^TATG
3719 CATATG
RcaI 1450 TCATGA T^CATGA
5512 TCATGA
SacI 284 GAGCTC GAGCT^C
1561 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I AspHI AspI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoII XmnI