################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## Acc65I AccI AclI AflII AflIII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AvrII BamHI BbrPI BciVI BclI BfrI BlnI BsaAI BsaBI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI Bst1107I BstZ17I ClaI CpoI DraIII EcoRV EcoT22I FbaI FseI KpnI MamI MfeI MluI MunI NcoI NdeI NotI NsiI PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SgrAI SnaBI SpeI SphI SspBI SspI SwaI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### AatII 3047 GACGTC GACGT^C AhdI 1914 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 1914 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BglII 6147 AGATCT A^GATCT BsmBI 96 GAGACG CGTCTC(1/5) BstBI 5710 TTCGAA TT^CGAA BstEII 485 GGTGACC G^GT[ACGT]ACC BstPI 485 GGTGACC G^GT[ACGT]ACC DraI 6272 TTTAAA TTT^AAA DrdI 2921 GACGGCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1211 CGGCCG C^GGCCG Eam1105I 1914 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1211 CGGCCG C^GGCCG Eco52I 1211 CGGCCG C^GGCCG EcoO65I 485 GGTGACC G^GT[ACGT]ACC EcoRI 4245 GAATTC G^AATTC HpaI 6232 GTTAAC GTT^AAC MslI 5785 CACCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NruI 3167 TCGCGA TCG^CGA NspV 5710 TTCGAA TT^CGAA PshAI 3156 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 5000 AGTACT AGT^ACT SexAI 4507 ACCAGGT A^CC[AT]GGT SfuI 5710 TTCGAA TT^CGAA XbaI 2597 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccIII 300 TCCGGA T^CCGGA 3784 TCCGGA AcsI 4245 GAATTC [GA]^AATT[CT] 6182 AAATTC AocI 1064 CCTAAGG CC^T[ACGT]AGG 5339 CCTCAGG ApoI 4245 GAATTC [GA]^AATT[CT] 6182 AAATTC Asp700I 647 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3177 GAAGAGCTTC AspI 1181 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2387 GACCCTGTC AviII 5546 TGCGCA TGC^GCA 5590 TGCGCA BbsI 2918 GAAGAC GAAGAC(2/6) 3163 GTCTTC BcgI 1943 CGAGAGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5702 GCAGGAGCTTCG BpuAI 2918 GAAGAC GAAGAC(2/6) 3163 GTCTTC BseAI 300 TCCGGA T^CCGGA 3784 TCCGGA BsiEI 1211 CGGCCG CG[GA][CT]^CG 4046 CGGTCG BsmI 413 GAATGC GAATGC(1/-1) 4149 GAATGC BspEI 300 TCCGGA T^CCGGA 3784 TCCGGA Bsu36I 1064 CCTAAGG CC^T[ACGT]AGG 5339 CCTCAGG Eco81I 1064 CCTAAGG CC^T[ACGT]AGG 5339 CCTCAGG FspI 5546 TGCGCA TGC^GCA 5590 TGCGCA HincII 963 GTCAAC GT[CT]^[GA]AC 6232 GTTAAC HindII 963 GTCAAC GT[CT]^[GA]AC 6232 GTTAAC MaeII 3048 ACGT A^CGT 5085 ACGT MroI 300 TCCGGA T^CCGGA 3784 TCCGGA NaeI 2020 GCCGGC GCC^GGC 3010 GCCGGC NgoMIV 2020 GCCGGC G^CCGGC 3010 GCCGGC NheI 2213 GCTAGC G^CTAGC 2327 GCTAGC PaeR7I 1866 CTCGAG C^TCGAG 4358 CTCGAG PflFI 1181 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2387 GACCCTGTC SmaI 4093 CCCGGG CCC^GGG 4942 CCCGGG Tsp509I 4246 AATT ^AATT 6183 AATT Tth111I 1181 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2387 GACCCTGTC XhoI 1866 CTCGAG C^TCGAG 4358 CTCGAG XmaCI 4093 CCCGGG C^CCGGG 4942 CCCGGG XmaI 4093 CCCGGG C^CCGGG 4942 CCCGGG XmnI 647 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3177 GAAGAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI Aor51HI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII SapI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SfiI SfoI SmlI Sse8387I StuI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII