##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AflIII      
AgeI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp718I     AvrII       BamHI       BbrPI       
BciVI       BclI        BfrI        BlnI        BsaAI       
BsaBI       BsiWI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrDI       BsrGI       Bst1107I    BstZ17I     
ClaI        CpoI        DraIII      EcoRV       EcoT22I     
FbaI        FseI        KpnI        MamI        MfeI        
MluI        MunI        NcoI        NdeI        NotI        
NsiI        PacI        PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SgrAI       SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AatII        3047  GACGTC               GACGT^C
AhdI         1914  GACTCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        1914  GACTCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglII        6147  AGATCT               A^GATCT
BsmBI          96  GAGACG               CGTCTC(1/5)
BstBI        5710  TTCGAA               TT^CGAA
BstEII        485  GGTGACC              G^GT[ACGT]ACC
BstPI         485  GGTGACC              G^GT[ACGT]ACC
DraI         6272  TTTAAA               TTT^AAA
DrdI         2921  GACGGCGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1211  CGGCCG               C^GGCCG
Eam1105I     1914  GACTCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1211  CGGCCG               C^GGCCG
Eco52I       1211  CGGCCG               C^GGCCG
EcoO65I       485  GGTGACC              G^GT[ACGT]ACC
EcoRI        4245  GAATTC               G^AATTC
HpaI         6232  GTTAAC               GTT^AAC
MslI         5785  CACCCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI         3167  TCGCGA               TCG^CGA
NspV         5710  TTCGAA               TT^CGAA
PshAI        3156  GACTCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI         5000  AGTACT               AGT^ACT
SexAI        4507  ACCAGGT              A^CC[AT]GGT
SfuI         5710  TTCGAA               TT^CGAA
XbaI         2597  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccIII        300  TCCGGA               T^CCGGA
             3784  TCCGGA              
AcsI         4245  GAATTC               [GA]^AATT[CT]
             6182  AAATTC              
AocI         1064  CCTAAGG              CC^T[ACGT]AGG
             5339  CCTCAGG             
ApoI         4245  GAATTC               [GA]^AATT[CT]
             6182  AAATTC              
Asp700I       647  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3177  GAAGAGCTTC          
AspI         1181  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2387  GACCCTGTC           
AviII        5546  TGCGCA               TGC^GCA
             5590  TGCGCA              
BbsI         2918  GAAGAC               GAAGAC(2/6)
             3163  GTCTTC              
BcgI         1943  CGAGAGCCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             5702  GCAGGAGCTTCG        
BpuAI        2918  GAAGAC               GAAGAC(2/6)
             3163  GTCTTC              
BseAI         300  TCCGGA               T^CCGGA
             3784  TCCGGA              
BsiEI        1211  CGGCCG               CG[GA][CT]^CG
             4046  CGGTCG              
BsmI          413  GAATGC               GAATGC(1/-1)
             4149  GAATGC              
BspEI         300  TCCGGA               T^CCGGA
             3784  TCCGGA              
Bsu36I       1064  CCTAAGG              CC^T[ACGT]AGG
             5339  CCTCAGG             
Eco81I       1064  CCTAAGG              CC^T[ACGT]AGG
             5339  CCTCAGG             
FspI         5546  TGCGCA               TGC^GCA
             5590  TGCGCA              
HincII        963  GTCAAC               GT[CT]^[GA]AC
             6232  GTTAAC              
HindII        963  GTCAAC               GT[CT]^[GA]AC
             6232  GTTAAC              
MaeII        3048  ACGT                 A^CGT
             5085  ACGT                
MroI          300  TCCGGA               T^CCGGA
             3784  TCCGGA              
NaeI         2020  GCCGGC               GCC^GGC
             3010  GCCGGC              
NgoMIV       2020  GCCGGC               G^CCGGC
             3010  GCCGGC              
NheI         2213  GCTAGC               G^CTAGC
             2327  GCTAGC              
PaeR7I       1866  CTCGAG               C^TCGAG
             4358  CTCGAG              
PflFI        1181  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2387  GACCCTGTC           
SmaI         4093  CCCGGG               CCC^GGG
             4942  CCCGGG              
Tsp509I      4246  AATT                 ^AATT
             6183  AATT                
Tth111I      1181  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2387  GACCCTGTC           
XhoI         1866  CTCGAG               C^TCGAG
             4358  CTCGAG              
XmaCI        4093  CCCGGG               C^CCGGG
             4942  CCCGGG              
XmaI         4093  CCCGGG               C^CCGGG
             4942  CCCGGG              
XmnI          647  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3177  GAAGAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  136 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       Aor51HI     ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssHII      BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco47III    EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     KspI        
MaeI        MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        SacII       SapI        Sau3AI      
Sau96I      SbfI        ScrFI       SfaNI       SfcI        
SfiI        SfoI        SmlI        Sse8387I    StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XcmI        
XhoII