##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AccI AflII AhdI AscI Asp700I
AspEI BbeI BbrPI BfrI BsiWI
Bsp1407I BspDI BsrGI BssHII Bst1107I
BstZ17I ClaI CpoI Eam1105I HindIII
HpaI KasI KspI MfeI MunI
NarI NcoI NdeI NotI NruI
PacI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI ScaI
SfoI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 2066 GACGTC GACGT^C
Acc65I 1252 GGTACC G^GTACC
AccIII 1915 TCCGGA T^CCGGA
AclI 876 AACGTT AA^CGTT
Alw44I 1871 GTGCAC G^TGCAC
ApaLI 1871 GTGCAC G^TGCAC
AseI 6268 ATTAAT AT^TAAT
AsnI 6268 ATTAAT AT^TAAT
Asp718I 1252 GGTACC G^GTACC
AspI 1695 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4210 TGCGCA TGC^GCA
AvrII 5425 CCTAGG C^CTAGG
BglII 3576 AGATCT A^GATCT
BlnI 5425 CCTAGG C^CTAGG
BseAI 1915 TCCGGA T^CCGGA
BspEI 1915 TCCGGA T^CCGGA
BspLU11I 1939 ACATGT A^CATGT
BsrBI 461 CCGCTC CCGCTC(-3/-3)
BsrDI 4521 GCAATG GCAATG(2/0)
BstBI 5019 TTCGAA TT^CGAA
DrdI 2060 GACCAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 145 CGGCCG C^GGCCG
EclXI 145 CGGCCG C^GGCCG
Eco52I 145 CGGCCG C^GGCCG
EcoRV 4011 GATATC GAT^ATC
FspI 4210 TGCGCA TGC^GCA
KpnI 1252 GGTACC GGTAC^C
MluI 4609 ACGCGT A^CGCGT
MroI 1915 TCCGGA T^CCGGA
NspV 5019 TTCGAA TT^CGAA
PaeR7I 3984 CTCGAG C^TCGAG
PflFI 1695 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 6268 ATTAAT AT^TAAT
Psp1406I 876 AACGTT AA^CGTT
SacI 3251 GAGCTC GAGCT^C
SexAI 631 ACCTGGT A^CC[AT]GGT
SfuI 5019 TTCGAA TT^CGAA
SphI 4399 GCATGC GCATG^C
Tth111I 1695 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5631 TCTAGA T^CTAGA
XhoI 3984 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AgeI 3911 ACCGGT A^CCGGT
6137 ACCGGT
Aor51HI 391 AGCGCT AGC^GCT
5465 AGCGCT
ApaI 5375 GGGCCC GGGCC^C
5872 GGGCCC
BciVI 3585 GGATAC GTATCC(6/5)
3594 GTATCC
BclI 1989 TGATCA T^GATCA
3435 TGATCA
BsaAI 889 TACGTG [CT]AC^GT[GA]
1604 TACGTG
BseRI 1257 CTCCTC GAGGAG(10/8)
1470 GAGGAG
BsiEI 145 CGGCCG CG[GA][CT]^CG
537 CGGTCG
BsmBI 1278 CGTCTC CGTCTC(1/5)
4553 CGTCTC
BsmI 1004 GAATGC GAATGC(1/-1)
3392 GCATTC
BspHI 5342 TCATGA T^CATGA
6129 TCATGA
BssSI 1488 CTCGTG CACGAG(-5/-1)
4699 CTCGTG
BstXI 4724 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5473 CCAGGAAGGTGG
DraI 4458 TTTAAA TTT^AAA
5171 TTTAAA
DraIII 1454 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1874 CACGACGTG
Eco47III 391 AGCGCT AGC^GCT
5465 AGCGCT
EcoNI 757 CCTCGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1449 CCTCCCACAGG
EcoRI 3166 GAATTC G^AATTC
3667 GAATTC
EcoT22I 1891 ATGCAT ATGCA^T
4401 ATGCAT
FbaI 1989 TGATCA T^GATCA
3435 TGATCA
FseI 125 GGCCGGCC GGCCGG^CC
6049 GGCCGGCC
HaeII 391 AGCGCT [GA]GCGC^[CT]
5465 AGCGCT
HincII 1292 GTCAAC GT[CT]^[GA]AC
2732 GTTGAC
HindII 1292 GTCAAC GT[CT]^[GA]AC
2732 GTTGAC
NaeI 126 GCCGGC GCC^GGC
6050 GCCGGC
NgoMIV 126 GCCGGC G^CCGGC
6050 GCCGGC
NheI 3224 GCTAGC G^CTAGC
5468 GCTAGC
NsiI 1891 ATGCAT ATGCA^T
4401 ATGCAT
PflMI 1766 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2098 CCAGAGACTGG
PinAI 3911 ACCGGT A^CCGGT
6137 ACCGGT
PshAI 2213 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2785 GACAGAGGTC
RcaI 5342 TCATGA T^CATGA
6129 TCATGA
SapI 3253 GCTCTTC GCTCTTC(1/4)
4228 GCTCTTC
Van91I 1766 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2098 CCAGAGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaJI BsaWI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI