##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AflIII AgeI AscI
Asp718I BamHI BclI BsiEI BsiWI
BspDI BspHI BspLU11I BssSI BstBI
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRV FbaI FseI KpnI
KspI MfeI MluI MunI NaeI
NcoI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmeI PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 3116 TCCGGA T^CCGGA
AclI 5293 AACGTT AA^CGTT
AhdI 4723 GACGTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4596 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3668 AGCGCT AGC^GCT
AseI 4464 ATTAAT AT^TAAT
AsnI 4464 ATTAAT AT^TAAT
AspEI 4723 GACGTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5725 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3395 TGCGCA TGC^GCA
AvrII 2382 CCTAGG C^CTAGG
BbeI 2343 GGCGCC GGCGC^C
BbrPI 644 CACGTG CAC^GTG
BlnI 2382 CCTAGG C^CTAGG
BseAI 3116 TCCGGA T^CCGGA
BsmBI 3649 CGTCTC CGTCTC(1/5)
Bsp1407I 1317 TGTACA T^GTACA
BspEI 3116 TCCGGA T^CCGGA
BsrDI 2731 GCAATG GCAATG(2/0)
BsrGI 1317 TGTACA T^GTACA
BssHII 899 GCGCGC G^CGCGC
Bst1107I 5020 GTATAC GTA^TAC
BstEII 4289 GGTCACC G^GT[ACGT]ACC
BstPI 4289 GGTCACC G^GT[ACGT]ACC
BstZ17I 5020 GTATAC GTA^TAC
Bsu36I 4596 CCTCAGG CC^T[ACGT]AGG
Eam1105I 4723 GACGTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3668 AGCGCT AGC^GCT
Eco81I 4596 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4289 GGTCACC G^GT[ACGT]ACC
EcoT22I 2614 ATGCAT ATGCA^T
FspI 3395 TGCGCA TGC^GCA
HindIII 5958 AAGCTT A^AGCTT
KasI 2343 GGCGCC G^GCGCC
MroI 3116 TCCGGA T^CCGGA
NarI 2343 GGCGCC GG^CGCC
NheI 1808 GCTAGC G^CTAGC
NsiI 2614 ATGCAT ATGCA^T
PflFI 5725 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 644 CACGTG CAC^GTG
PmlI 644 CACGTG CAC^GTG
PshAI 3376 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4464 ATTAAT AT^TAAT
Psp1406I 5293 AACGTT AA^CGTT
SacI 940 GAGCTC GAGCT^C
SapI 5801 GCTCTTC GCTCTTC(1/4)
ScaI 4254 AGTACT AGT^ACT
SfoI 2343 GGCGCC GGC^GCC
SmaI 1667 CCCGGG CCC^GGG
SphI 1404 GCATGC GCATG^C
SspBI 1317 TGTACA T^GTACA
SwaI 6309 ATTTAAAT ATTT^AAAT
Tth111I 5725 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1667 CCCGGG C^CCGGG
XmaI 1667 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccII 900 CGCG CG^CG
2332 CGCG
Alw44I 5395 GTGCAC G^TGCAC
5600 GTGCAC
ApaI 431 GGGCCC GGGCC^C
1644 GGGCCC
ApaLI 5395 GTGCAC G^TGCAC
5600 GTGCAC
BcgI 3313 GCATCAGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4540 GCAGTCTCTTCG
BsaAI 644 CACGTG [CT]AC^GT[GA]
1868 TACGTG
BsaBI 1198 GATCCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5032 GATGCCCATC
BsrFI 4060 ACCGGC [GA]^CCGG[CT]
6199 GCCGGT
BstUI 900 CGCG CG^CG
2332 CGCG
Cfr10I 4060 ACCGGC [GA]^CCGG[CT]
6199 GCCGGT
EcoRI 5155 GAATTC G^AATTC
6298 GAATTC
MamI 1198 GATCCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5032 GATGCCCATC
MvnI 900 CGCG CG^CG
2332 CGCG
NdeI 399 CATATG CA^TATG
6289 CATATG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BfrI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI