##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AclI AgeI Alw44I Aor51HI ApaI
ApaLI AscI AviII BbrPI BbvCI
BcgI BsaAI BsiEI BsiWI BsmBI
BspDI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI EagI EclXI Eco47III Eco52I
FseI FspI KspI MluI NaeI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SfiI SfuI SgrAI SmaI
SnaBI SphI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 882 GGTACC G^GTACC
AccIII 5928 TCCGGA T^CCGGA
AflII 5174 CTTAAG C^TTAAG
AflIII 6183 ACATGT A^C[GA][CT]GT
AseI 5336 ATTAAT AT^TAAT
AsnI 5336 ATTAAT AT^TAAT
Asp718I 882 GGTACC G^GTACC
AvrII 3611 CCTAGG C^CTAGG
BamHI 3911 GGATCC G^GATCC
BbeI 3574 GGCGCC GGCGC^C
BciVI 6438 GTATCC GTATCC(6/5)
BfrI 5174 CTTAAG C^TTAAG
BlnI 3611 CCTAGG C^CTAGG
BlpI 2207 GCTCAGC GC^T[ACGT]AGC
BpmI 3127 CTCCAG CTGGAG(16/14)
Bpu1102I 2207 GCTCAGC GC^T[ACGT]AGC
BseAI 5928 TCCGGA T^CCGGA
BspEI 5928 TCCGGA T^CCGGA
BspHI 1927 TCATGA T^CATGA
BspLU11I 6183 ACATGT A^CATGT
BstEII 173 GGTGACC G^GT[ACGT]ACC
BstPI 173 GGTGACC G^GT[ACGT]ACC
CelII 2207 GCTCAGC GC^T[ACGT]AGC
DraIII 2014 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 173 GGTGACC G^GT[ACGT]ACC
EcoRI 4285 GAATTC G^AATTC
EcoRV 243 GATATC GAT^ATC
HaeII 3574 GGCGCC [GA]GCGC^[CT]
KasI 3574 GGCGCC G^GCGCC
KpnI 882 GGTACC GGTAC^C
MfeI 406 CAATTG C^AATTG
MroI 5928 TCCGGA T^CCGGA
MunI 406 CAATTG C^AATTG
NarI 3574 GGCGCC GG^CGCC
NheI 4987 GCTAGC G^CTAGC
PshBI 5336 ATTAAT AT^TAAT
RcaI 1927 TCATGA T^CATGA
SacI 4811 GAGCTC GAGCT^C
SbfI 3915 CCTGCAGG CCTGCA^GG
SexAI 5744 ACCTGGT A^CC[AT]GGT
SfoI 3574 GGCGCC GGC^GCC
SpeI 213 ACTAGT A^CTAGT
Sse8387I 3915 CCTGCAGG CCTGCA^GG
SspI 2008 AATATT AAT^ATT
XcmI 3460 CCAGTGGTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 3809 GACGTC GACGT^C
4616 GACGTC
AccI 2724 GTAGAC GT^[AC][GT]AC
6140 GTCTAC
AlwNI 3165 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
3484 CAGATTCTG
AspHI 4605 GAGCAC G[AT]GC[AT]^C
4811 GAGCTC
AspI 479 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5460 GACGGTGTC
BcnI 2000 CCCGG CC^[GC]GG
4272 CCCGG
BglI 2973 GCCCAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4418 GCCACATGGGC
BglII 1571 AGATCT A^GATCT
2576 AGATCT
BsaWI 412 ACCGGA [AT]^CCGG[AT]
5928 TCCGGA
BsiHKAI 4605 GAGCAC G[AT]GC[AT]^C
4811 GAGCTC
BstAPI 452 GCACCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6194 GCATTATTTGC
DrdI 2727 GACGGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5425 GACCCTTGTGTC
HindIII 2958 AAGCTT A^AGCTT
3307 AAGCTT
HpaI 896 GTTAAC GTT^AAC
1223 GTTAAC
NciI 2000 CCCGG CC^[GC]GG
4272 CCCGG
NcoI 89 CCATGG C^CATGG
673 CCATGG
NdeI 47 CATATG CA^TATG
3942 CATATG
PflFI 479 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5460 GACGGTGTC
PflMI 3282 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5368 CCAGTGGATGG
SapI 564 GAAGAGC GCTCTTC(1/4)
915 GAAGAGC
ScaI 4209 AGTACT AGT^ACT
5958 AGTACT
StuI 4443 AGGCCT AGG^CCT
5831 AGGCCT
Tth111I 479 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5460 GACGGTGTC
Van91I 3282 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5368 CCAGTGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AocI ApoI
Asp700I AspEI AvaI AvaII BalI
BanI BanII BbsI BbvI BclI
BfaI BmyI BpuAI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI