##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Aor51HI     AscI        
Asp718I     AspEI       BcgI        BciVI       BclI        
BfrI        BsaBI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       FbaI        FseI        HpaI        
KpnI        KspI        MamI        MluI        MroI        
NotI        NruI        NspV        PacI        PinAI       
PmeI        Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

Alw44I       2884  GTGCAC               G^TGCAC
ApaLI        2884  GTGCAC               G^TGCAC
AseI         3595  ATTAAT               AT^TAAT
AsnI         3595  ATTAAT               AT^TAAT
AviII         866  TGCGCA               TGC^GCA
BbeI         1592  GGCGCC               GGCGC^C
BbvCI        3234  GCTGAGG              CCTCAGC(-5/-2)
BglII        3223  AGATCT               A^GATCT
BsmBI        1892  CGTCTC               CGTCTC(1/5)
Bsp1407I     5374  TGTACA               T^GTACA
BspLU11I     2836  ACATGT               A^CATGT
BsrGI        5374  TGTACA               T^GTACA
BssSI        2143  CTCGTG               CACGAG(-5/-1)
BstAPI       2886  GCACAGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII       3297  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1285  GACATTATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1296  CCTAAGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      2248  ATGCAT               ATGCA^T
FspI          866  TGCGCA               TGC^GCA
KasI         1592  GGCGCC               G^GCGCC
MfeI         2374  CAATTG               C^AATTG
MunI         2374  CAATTG               C^AATTG
NarI         1592  GGCGCC               GG^CGCC
NheI          789  GCTAGC               G^CTAGC
NsiI         2248  ATGCAT               ATGCA^T
PaeR7I       1223  CTCGAG               C^TCGAG
PflMI         690  CCACAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        4463  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3595  ATTAAT               AT^TAAT
SfoI         1592  GGCGCC               GGC^GCC
SspBI        5374  TGTACA               T^GTACA
StuI         2514  AGGCCT               AGG^CCT
SwaI         2447  ATTTAAAT             ATTT^AAAT
Van91I        690  CCACAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1223  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         117  CGCG                 CG^CG
              583  CGCG                
AflIII        552  ACGTGT               A^C[GA][CT]GT
             2836  ACATGT              
AocI         4597  CCTCAGG              CC^T[ACGT]AGG
             4926  CCTTAGG             
ApaI          411  GGGCCC               GGGCC^C
             4700  GGGCCC              
AspI          273  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1648  GACCCTGTC           
AvrII        3710  CCTAGG               C^CTAGG
             5262  CCTAGG              
BbrPI         551  CACGTG               CAC^GTG
             4729  CACGTG              
BglI         3138  GCCACTTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4693  GCCCCTTGGGC         
BlnI         3710  CCTAGG               C^CTAGG
             5262  CCTAGG              
BsaWI         599  ACCGGA               [AT]^CCGG[AT]
             4655  ACCGGA              
BstUI         117  CGCG                 CG^CG
              583  CGCG                
BstXI        3493  CCATGGCACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5190  CCAGGTACTTGG        
Bsu36I       4597  CCTCAGG              CC^T[ACGT]AGG
             4926  CCTTAGG             
Eco81I       4597  CCTCAGG              CC^T[ACGT]AGG
             4926  CCTTAGG             
EcoRI        2281  GAATTC               G^AATTC
             4769  GAATTC              
MvnI          117  CGCG                 CG^CG
              583  CGCG                
NaeI         1442  GCCGGC               GCC^GGC
             3779  GCCGGC              
NgoMIV       1442  GCCGGC               G^CCGGC
             3779  GCCGGC              
PflFI         273  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1648  GACCCTGTC           
PmaCI         551  CACGTG               CAC^GTG
             4729  CACGTG              
PmlI          551  CACGTG               CAC^GTG
             4729  CACGTG              
SapI         2043  GCTCTTC              GCTCTTC(1/4)
             3165  GCTCTTC             
ScaI         1144  AGTACT               AGT^ACT
             2258  AGTACT              
Tth111I       273  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1648  GACCCTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  130 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BamHI       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspHI       
BspMI       BsrBI       BsrDI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RcaI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI