##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AviII BbeI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BssHII BssSI ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FseI FspI HaeII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
PacI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1282 GTATAC GT^[AC][GT]AC
AcyI 2163 GGCGTC G[GA]^CG[CT]C
Alw44I 3298 GTGCAC G^TGCAC
ApaLI 3298 GTGCAC G^TGCAC
AseI 1208 ATTAAT AT^TAAT
AsnI 1208 ATTAAT AT^TAAT
AvrII 5656 CCTAGG C^CTAGG
BalI 2004 TGGCCA TGG^CCA
BcgI 1707 GCAGGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1461 GGATAC GTATCC(6/5)
BcnI 3294 CCCGG CC^[GC]GG
BglI 2136 GCCCCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5656 CCTAGG C^CTAGG
BsaBI 2416 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2163 GGCGTC G[GA]^CG[CT]C
BseRI 2603 GAGGAG GAGGAG(10/8)
BspHI 1251 TCATGA T^CATGA
BsrFI 2788 GCCGGT [GA]^CCGG[CT]
Bst1107I 1282 GTATAC GTA^TAC
BstAPI 2213 GCAGTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2307 TTCGAA TT^CGAA
BstEII 397 GGTCACC G^GT[ACGT]ACC
BstPI 397 GGTCACC G^GT[ACGT]ACC
BstZ17I 1282 GTATAC GTA^TAC
Cfr10I 2788 GCCGGT [GA]^CCGG[CT]
DrdI 3721 GACCTTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 397 GGTCACC G^GT[ACGT]ACC
FauI 2785 CCCGC CCCGC(4/6)
Hin1I 2163 GGCGTC G[GA]^CG[CT]C
MamI 2416 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2004 TGGCCA TGG^CCA
MscI 2004 TGGCCA TGG^CCA
NciI 3294 CCCGG CC^[GC]GG
NheI 1160 GCTAGC G^CTAGC
NspV 2307 TTCGAA TT^CGAA
PaeR7I 678 CTCGAG C^TCGAG
PshAI 773 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1208 ATTAAT AT^TAAT
RcaI 1251 TCATGA T^CATGA
ScaI 551 AGTACT AGT^ACT
SexAI 401 ACCAGGT A^CC[AT]GGT
SfuI 2307 TTCGAA TT^CGAA
SspI 6490 AATATT AAT^ATT
SwaI 1785 ATTTAAAT ATTT^AAAT
XhoI 678 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 1984 CGCG CG^CG
2727 CGCG
AflIII 454 ACGTGT A^C[GA][CT]GT
4894 ACACGT
BamHI 1106 GGATCC G^GATCC
5875 GGATCC
BanI 409 GGCACC G^G[CT][GA]CC
5563 GGTGCC
BbrPI 453 CACGTG CAC^GTG
3370 CACGTG
BbsI 5402 GAAGAC GAAGAC(2/6)
987 GTCTTC
BbvCI 2460 CCTCAGC CCTCAGC(-5/-2)
3506 CCTCAGC
BlpI 810 GCTGAGC GC^T[ACGT]AGC
1475 GCTAAGC
Bpu1102I 810 GCTGAGC GC^T[ACGT]AGC
1475 GCTAAGC
BpuAI 5402 GAAGAC GAAGAC(2/6)
987 GTCTTC
Bsp1407I 5766 TGTACA T^GTACA
6080 TGTACA
BsrGI 5766 TGTACA T^GTACA
6080 TGTACA
BstUI 1984 CGCG CG^CG
2727 CGCG
CelII 810 GCTGAGC GC^T[ACGT]AGC
1475 GCTAAGC
EaeI 2004 TGGCCA [CT]^GGCC[GA]
4776 TGGCCG
EcoNI 1691 CCTTTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5917 CCTGGGGTAGG
EcoRI 1730 GAATTC G^AATTC
5007 GAATTC
HapII 2789 CCGG C^CGG
3295 CCGG
HincII 4455 GTTGAC GT[CT]^[GA]AC
5293 GTTGAC
HindII 4455 GTTGAC GT[CT]^[GA]AC
5293 GTTGAC
HindIII 5061 AAGCTT A^AGCTT
5523 AAGCTT
HpaII 2789 CCGG C^CGG
3295 CCGG
MspI 2789 CCGG C^CGG
3295 CCGG
MvnI 1984 CGCG CG^CG
2727 CGCG
NcoI 913 CCATGG C^CATGG
2513 CCATGG
PmaCI 453 CACGTG CAC^GTG
3370 CACGTG
PmlI 453 CACGTG CAC^GTG
3370 CACGTG
SpeI 2386 ACTAGT A^CTAGT
4691 ACTAGT
SspBI 5766 TGTACA T^GTACA
6080 TGTACA
StuI 4558 AGGCCT AGG^CCT
4951 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AspI AvaI AvaII BanII
BbvI BclI BfaI BfrI BglII
BmyI BpmI BsaAI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EarI Eco81I
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HgaI HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmnI