################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AclI AflII AgeI AhdI AscI AspEI AviII AvrII BcgI BfrI BlnI BsiEI BsiWI Bsp1407I BspHI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I CpoI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FseI FspI HpaI KspI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RcaI RsrII SacII SalI SexAI SfuI SgrAI SnaBI SpeI SphI SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 163 CGCG CG^CG Alw44I 5220 GTGCAC G^TGCAC Aor51HI 5115 AGCGCT AGC^GCT ApaLI 5220 GTGCAC G^TGCAC Asp700I 5631 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2843 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 5223 CACGTG CAC^GTG BciVI 5276 GTATCC GTATCC(6/5) BclI 498 TGATCA T^GATCA BglII 3737 AGATCT A^GATCT BsaAI 5223 CACGTG [CT]AC^GT[GA] BsmBI 1278 GAGACG CGTCTC(1/5) BspDI 3195 ATCGAT AT^CGAT BspLU11I 4738 ACATGT A^CATGT BsrFI 5820 GCCGGT [GA]^CCGG[CT] BssSI 1674 CACGAG CACGAG(-5/-1) BstEII 1556 GGTCACC G^GT[ACGT]ACC BstPI 1556 GGTCACC G^GT[ACGT]ACC BstUI 163 CGCG CG^CG Cfr10I 5820 GCCGGT [GA]^CCGG[CT] ClaI 3195 ATCGAT AT^CGAT DraI 6158 TTTAAA TTT^AAA Eco47III 5115 AGCGCT AGC^GCT EcoO65I 1556 GGTCACC G^GT[ACGT]ACC FbaI 498 TGATCA T^GATCA HindIII 4240 AAGCTT A^AGCTT MvnI 163 CGCG CG^CG PflFI 2843 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 5223 CACGTG CAC^GTG PmlI 5223 CACGTG CAC^GTG PshAI 1550 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 545 GAAGAGC GCTCTTC(1/4) SbfI 1396 CCTGCAGG CCTGCA^GG SfiI 5312 GGCCCCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 1396 CCTGCAGG CCTGCA^GG SspI 3222 AATATT AAT^ATT StuI 4947 AGGCCT AGG^CCT Tth111I 2843 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 5069 CCAGCAGCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 5631 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Acc65I 1861 GGTACC G^GTACC 6063 GGTACC AccI 3539 GTAGAC GT^[AC][GT]AC 4158 GTAGAC AflIII 3176 ACACGT A^C[GA][CT]GT 4738 ACATGT AlwNI 2060 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 3747 CAGGCCCTG Asp718I 1861 GGTACC G^GTACC 6063 GGTACC BamHI 1402 GGATCC G^GATCC 2931 GGATCC BbvCI 1648 CCTCAGC CCTCAGC(-5/-2) 2355 CCTCAGC BsaBI 1804 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5271 GATGTGTATC BsrBI 557 GAGCGG CCGCTC(-3/-3) 2045 GAGCGG KpnI 1861 GGTACC GGTAC^C 6063 GGTACC MamI 1804 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5271 GATGTGTATC MslI 1528 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4384 CATCCTAATG PflMI 4297 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5683 CCACCACCTGG ScaI 1539 AGTACT AGT^ACT 3555 AGTACT SmaI 649 CCCGGG CCC^GGG 2034 CCCGGG TaqI 3181 TCGA T^CGA 3196 TCGA TthHB8I 3181 TCGA T^CGA 3196 TCGA Van91I 4297 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5683 CCACCACCTGG XmaCI 649 CCCGGG C^CCGGG 2034 CCCGGG XmaI 649 CCCGGG C^CCGGG 2034 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccIII AciI AcsI AcyI AfaI AluI AlwI AocI ApaI ApoI AseI AsnI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspEI BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MspA1I MspI MvaI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI XhoII