##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       AclI        AflII       AgeI        AhdI        
AscI        AspEI       AviII       AvrII       BcgI        
BfrI        BlnI        BsiEI       BsiWI       Bsp1407I    
BspHI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstZ17I     CpoI        EagI        Eam1105I    
EclXI       Eco52I      EcoRI       EcoRV       EcoT22I     
FseI        FspI        HpaI        KspI        MfeI        
MluI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
SphI        SspBI       SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccII         163  CGCG                 CG^CG
Alw44I       5220  GTGCAC               G^TGCAC
Aor51HI      5115  AGCGCT               AGC^GCT
ApaLI        5220  GTGCAC               G^TGCAC
Asp700I      5631  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         2843  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        5223  CACGTG               CAC^GTG
BciVI        5276  GTATCC               GTATCC(6/5)
BclI          498  TGATCA               T^GATCA
BglII        3737  AGATCT               A^GATCT
BsaAI        5223  CACGTG               [CT]AC^GT[GA]
BsmBI        1278  GAGACG               CGTCTC(1/5)
BspDI        3195  ATCGAT               AT^CGAT
BspLU11I     4738  ACATGT               A^CATGT
BsrFI        5820  GCCGGT               [GA]^CCGG[CT]
BssSI        1674  CACGAG               CACGAG(-5/-1)
BstEII       1556  GGTCACC              G^GT[ACGT]ACC
BstPI        1556  GGTCACC              G^GT[ACGT]ACC
BstUI         163  CGCG                 CG^CG
Cfr10I       5820  GCCGGT               [GA]^CCGG[CT]
ClaI         3195  ATCGAT               AT^CGAT
DraI         6158  TTTAAA               TTT^AAA
Eco47III     5115  AGCGCT               AGC^GCT
EcoO65I      1556  GGTCACC              G^GT[ACGT]ACC
FbaI          498  TGATCA               T^GATCA
HindIII      4240  AAGCTT               A^AGCTT
MvnI          163  CGCG                 CG^CG
PflFI        2843  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        5223  CACGTG               CAC^GTG
PmlI         5223  CACGTG               CAC^GTG
PshAI        1550  GACCGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI          545  GAAGAGC              GCTCTTC(1/4)
SbfI         1396  CCTGCAGG             CCTGCA^GG
SfiI         5312  GGCCCCCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I     1396  CCTGCAGG             CCTGCA^GG
SspI         3222  AATATT               AAT^ATT
StuI         4947  AGGCCT               AGG^CCT
Tth111I      2843  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         5069  CCAGCAGCATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         5631  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Acc65I       1861  GGTACC               G^GTACC
             6063  GGTACC              
AccI         3539  GTAGAC               GT^[AC][GT]AC
             4158  GTAGAC              
AflIII       3176  ACACGT               A^C[GA][CT]GT
             4738  ACATGT              
AlwNI        2060  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3747  CAGGCCCTG           
Asp718I      1861  GGTACC               G^GTACC
             6063  GGTACC              
BamHI        1402  GGATCC               G^GATCC
             2931  GGATCC              
BbvCI        1648  CCTCAGC              CCTCAGC(-5/-2)
             2355  CCTCAGC             
BsaBI        1804  GATGGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             5271  GATGTGTATC          
BsrBI         557  GAGCGG               CCGCTC(-3/-3)
             2045  GAGCGG              
KpnI         1861  GGTACC               GGTAC^C
             6063  GGTACC              
MamI         1804  GATGGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             5271  GATGTGTATC          
MslI         1528  CATTGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             4384  CATCCTAATG          
PflMI        4297  CCACTGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5683  CCACCACCTGG         
ScaI         1539  AGTACT               AGT^ACT
             3555  AGTACT              
SmaI          649  CCCGGG               CCC^GGG
             2034  CCCGGG              
TaqI         3181  TCGA                 T^CGA
             3196  TCGA                
TthHB8I      3181  TCGA                 T^CGA
             3196  TCGA                
Van91I       4297  CCACTGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5683  CCACCACCTGG         
XmaCI         649  CCCGGG               C^CCGGG
             2034  CCCGGG              
XmaI          649  CCCGGG               C^CCGGG
             2034  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccIII      AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AocI        ApaI        ApoI        
AseI        AsnI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbeI        BbsI        
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspEI       
BspMI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DraIII      DrdI        DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MroI        MscI        MseI        
MspA1I      MspI        MvaI        MwoI        NarI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PshBI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmlI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XbaI        XhoII