##################################
# NO CUT ENZYMES ( 31 enzymes) #
##################################
AclI AgeI AscI AseI AsnI
AvrII BlnI BsiWI BssHII Bst1107I
BstZ17I CpoI FseI KspI MluI
NotI PacI PinAI PmeI PshBI
Psp1406I RsrII SacII SbfI SfiI
SgrAI SnaBI SpeI Sse8387I SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 14 enzymes) #
###################################
AatII 295 GACGTC GACGT^C
Acc65I 3044 GGTACC G^GTACC
AflII 2233 CTTAAG C^TTAAG
Asp718I 3044 GGTACC G^GTACC
BfrI 2233 CTTAAG C^TTAAG
BstBI 13906 TTCGAA TT^CGAA
EcoT22I 1240 ATGCAT ATGCA^T
KpnI 3044 GGTACC GGTAC^C
NheI 9516 GCTAGC G^CTAGC
NruI 1839 TCGCGA TCG^CGA
NsiI 1240 ATGCAT ATGCA^T
NspV 13906 TTCGAA TT^CGAA
PvuI 12030 CGATCG CGAT^CG
SfuI 13906 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Alw44I 10910 GTGCAC G^TGCAC
13965 GTGCAC
AocI 1485 CCTGAGG CC^T[ACGT]AGG
13585 CCTGAGG
ApaI 6706 GGGCCC GGGCC^C
8253 GGGCCC
ApaLI 10910 GTGCAC G^TGCAC
13965 GTGCAC
BspDI 4063 ATCGAT AT^CGAT
7639 ATCGAT
BspHI 2913 TCATGA T^CATGA
4283 TCATGA
BssSI 9175 CACGAG CACGAG(-5/-1)
9598 CACGAG
Bsu36I 1485 CCTGAGG CC^T[ACGT]AGG
13585 CCTGAGG
ClaI 4063 ATCGAT AT^CGAT
7639 ATCGAT
Eco81I 1485 CCTGAGG CC^T[ACGT]AGG
13585 CCTGAGG
HpaI 781 GTTAAC GTT^AAC
2245 GTTAAC
NdeI 2528 CATATG CA^TATG
6611 CATATG
PaeR7I 6766 CTCGAG C^TCGAG
11651 CTCGAG
RcaI 2913 TCATGA T^CATGA
4283 TCATGA
SalI 9670 GTCGAC G^TCGAC
12898 GTCGAC
XhoI 6766 CTCGAG C^TCGAG
11651 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 197 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AhdI AluI
AlwI AlwNI Aor51HI ApoI Asp700I
AspEI AspHI AspI AvaI AvaII
AviII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT14I
FauI FbaI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MunI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PmaCI PmlI
PpuMI PshAI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SexAI SfaNI SfcI SfoI
SmaI SmlI SphI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI