##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspI        AviII       BbeI        BsaHI       BsiWI       
BssHII      CpoI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoRV       FseI        FspI        
HgaI        Hin1I       KasI        KpnI        KspI        
MfeI        MluI        MunI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NruI        
PacI        PflFI       PshAI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SpeI        SphI        Sse8387I    StuI        SwaI        
Tth111I     XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII        3572  CGCG                 CG^CG
AflII         578  CTTAAG               C^TTAAG
AgeI         5621  ACCGGT               A^CCGGT
AhdI         2678  GACCTTCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         5630  CCTGAGG              CC^T[ACGT]AGG
AseI         3461  ATTAAT               AT^TAAT
AsnI         3461  ATTAAT               AT^TAAT
AspEI        2678  GACCTTCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        2362  CCTAGG               C^CTAGG
BamHI        3710  GGATCC               G^GATCC
BbrPI        2484  CACGTG               CAC^GTG
BbvCI        4444  CCTCAGC              CCTCAGC(-5/-2)
BcgI         6025  CGAGCGGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          578  CTTAAG               C^TTAAG
BglI          834  GCCGGAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         2362  CCTAGG               C^CTAGG
BlpI         6619  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     6619  GCTGAGC              GC^T[ACGT]AGC
BsaBI         275  GATAGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        5839  CGGTCG               CG[GA][CT]^CG
BsmBI        2685  CGTCTC               CGTCTC(1/5)
BsmI         1809  GAATGC               GAATGC(1/-1)
BspDI        3339  ATCGAT               AT^CGAT
BspHI        4320  TCATGA               T^CATGA
BsrFI        5621  ACCGGT               [GA]^CCGG[CT]
BssSI        1081  CTCGTG               CACGAG(-5/-1)
Bst1107I     6190  GTATAC               GTA^TAC
BstUI        3572  CGCG                 CG^CG
BstXI        2023  CCAGTGTACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      6190  GTATAC               GTA^TAC
Bsu36I       5630  CCTGAGG              CC^T[ACGT]AGG
CelII        6619  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       5621  ACCGGT               [GA]^CCGG[CT]
ClaI         3339  ATCGAT               AT^CGAT
DrdI         5578  GACTTCTTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2678  GACCTTCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       5630  CCTGAGG              CC^T[ACGT]AGG
HpaI         3785  GTTAAC               GTT^AAC
MamI          275  GATAGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         3572  CGCG                 CG^CG
PaeR7I       5508  CTCGAG               C^TCGAG
PinAI        5621  ACCGGT               A^CCGGT
PmaCI        2484  CACGTG               CAC^GTG
PmeI         6265  GTTTAAAC             GTTT^AAAC
PmlI         2484  CACGTG               CAC^GTG
PshBI        3461  ATTAAT               AT^TAAT
RcaI         4320  TCATGA               T^CATGA
SapI          212  GCTCTTC              GCTCTTC(1/4)
SnaBI        6187  TACGTA               TAC^GTA
XbaI         5711  TCTAGA               T^CTAGA
XhoI         5508  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccI         4797  GTAGAC               GT^[AC][GT]AC
             6190  GTATAC              
AccIII       1563  TCCGGA               T^CCGGA
             3667  TCCGGA              
AvaI         4136  CCCGAG               C^[CT]CG[GA]G
             5508  CTCGAG              
BciVI        1379  GGATAC               GTATCC(6/5)
             4483  GGATAC              
BclI          328  TGATCA               T^GATCA
             4279  TGATCA              
BsaAI        2484  CACGTG               [CT]AC^GT[GA]
             6187  TACGTA              
BseAI        1563  TCCGGA               T^CCGGA
             3667  TCCGGA              
BsoBI        4136  CCCGAG               C^[CT]CG[GA]G
             5508  CTCGAG              
Bsp1407I     2885  TGTACA               T^GTACA
             5440  TGTACA              
BspEI        1563  TCCGGA               T^CCGGA
             3667  TCCGGA              
BsrDI        3724  CATTGC               GCAATG(2/0)
             1264  GCAATG              
BsrGI        2885  TGTACA               T^GTACA
             5440  TGTACA              
BstBI        1806  TTCGAA               TT^CGAA
             3296  TTCGAA              
BstEII       3500  GGTAACC              G^GT[ACGT]ACC
             4558  GGTCACC             
BstPI        3500  GGTAACC              G^GT[ACGT]ACC
             4558  GGTCACC             
CfoI         2720  GCGC                 GCG^C
             5974  GCGC                
DraII        3154  AGGACCT              [GA]G^G[ACGT]CC[CT]
             3320  GGGACCC             
DsaI          726  CCACGG               C^C[GA][CT]GG
             1490  CCACGG              
EcoO109I     3154  AGGACCT              [GA]G^G[ACGT]CC[CT]
             3320  GGGACCC             
EcoO65I      3500  GGTAACC              G^GT[ACGT]ACC
             4558  GGTCACC             
EcoT22I      1975  ATGCAT               ATGCA^T
             2129  ATGCAT              
FbaI          328  TGATCA               T^GATCA
             4279  TGATCA              
HaeII        2719  AGCGCC               [GA]GCGC^[CT]
             5973  AGCGCC              
HhaI         2720  GCGC                 GCG^C
             5974  GCGC                
HinP1I       2720  GCGC                 G^CGC
             5974  GCGC                
HincII       1682  GTCAAC               GT[CT]^[GA]AC
             3785  GTTAAC              
HindII       1682  GTCAAC               GT[CT]^[GA]AC
             3785  GTTAAC              
MroI         1563  TCCGGA               T^CCGGA
             3667  TCCGGA              
NsiI         1975  ATGCAT               ATGCA^T
             2129  ATGCAT              
NspV         1806  TTCGAA               TT^CGAA
             3296  TTCGAA              
PpuMI        3154  AGGACCT              [GA]G^G[AT]CC[CT]
             3320  GGGACCC             
PvuII          27  CAGCTG               CAG^CTG
              183  CAGCTG              
SfuI         1806  TTCGAA               TT^CGAA
             3296  TTCGAA              
SspBI        2885  TGTACA               T^GTACA
             5440  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspLU11I    BspMI       
BsrBI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraIII      EaeI        EarI        
EcoRI       EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PstI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       XmnI