##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AcyI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspI AviII BbeI BsaHI BsiWI
BssHII CpoI EagI EclXI Eco47III
Eco52I EcoNI EcoRV FseI FspI
HgaI Hin1I KasI KpnI KspI
MfeI MluI MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
PacI PflFI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SpeI SphI Sse8387I StuI SwaI
Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 3572 CGCG CG^CG
AflII 578 CTTAAG C^TTAAG
AgeI 5621 ACCGGT A^CCGGT
AhdI 2678 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5630 CCTGAGG CC^T[ACGT]AGG
AseI 3461 ATTAAT AT^TAAT
AsnI 3461 ATTAAT AT^TAAT
AspEI 2678 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2362 CCTAGG C^CTAGG
BamHI 3710 GGATCC G^GATCC
BbrPI 2484 CACGTG CAC^GTG
BbvCI 4444 CCTCAGC CCTCAGC(-5/-2)
BcgI 6025 CGAGCGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 578 CTTAAG C^TTAAG
BglI 834 GCCGGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2362 CCTAGG C^CTAGG
BlpI 6619 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 6619 GCTGAGC GC^T[ACGT]AGC
BsaBI 275 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 5839 CGGTCG CG[GA][CT]^CG
BsmBI 2685 CGTCTC CGTCTC(1/5)
BsmI 1809 GAATGC GAATGC(1/-1)
BspDI 3339 ATCGAT AT^CGAT
BspHI 4320 TCATGA T^CATGA
BsrFI 5621 ACCGGT [GA]^CCGG[CT]
BssSI 1081 CTCGTG CACGAG(-5/-1)
Bst1107I 6190 GTATAC GTA^TAC
BstUI 3572 CGCG CG^CG
BstXI 2023 CCAGTGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 6190 GTATAC GTA^TAC
Bsu36I 5630 CCTGAGG CC^T[ACGT]AGG
CelII 6619 GCTGAGC GC^T[ACGT]AGC
Cfr10I 5621 ACCGGT [GA]^CCGG[CT]
ClaI 3339 ATCGAT AT^CGAT
DrdI 5578 GACTTCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2678 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5630 CCTGAGG CC^T[ACGT]AGG
HpaI 3785 GTTAAC GTT^AAC
MamI 275 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 3572 CGCG CG^CG
PaeR7I 5508 CTCGAG C^TCGAG
PinAI 5621 ACCGGT A^CCGGT
PmaCI 2484 CACGTG CAC^GTG
PmeI 6265 GTTTAAAC GTTT^AAAC
PmlI 2484 CACGTG CAC^GTG
PshBI 3461 ATTAAT AT^TAAT
RcaI 4320 TCATGA T^CATGA
SapI 212 GCTCTTC GCTCTTC(1/4)
SnaBI 6187 TACGTA TAC^GTA
XbaI 5711 TCTAGA T^CTAGA
XhoI 5508 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 4797 GTAGAC GT^[AC][GT]AC
6190 GTATAC
AccIII 1563 TCCGGA T^CCGGA
3667 TCCGGA
AvaI 4136 CCCGAG C^[CT]CG[GA]G
5508 CTCGAG
BciVI 1379 GGATAC GTATCC(6/5)
4483 GGATAC
BclI 328 TGATCA T^GATCA
4279 TGATCA
BsaAI 2484 CACGTG [CT]AC^GT[GA]
6187 TACGTA
BseAI 1563 TCCGGA T^CCGGA
3667 TCCGGA
BsoBI 4136 CCCGAG C^[CT]CG[GA]G
5508 CTCGAG
Bsp1407I 2885 TGTACA T^GTACA
5440 TGTACA
BspEI 1563 TCCGGA T^CCGGA
3667 TCCGGA
BsrDI 3724 CATTGC GCAATG(2/0)
1264 GCAATG
BsrGI 2885 TGTACA T^GTACA
5440 TGTACA
BstBI 1806 TTCGAA TT^CGAA
3296 TTCGAA
BstEII 3500 GGTAACC G^GT[ACGT]ACC
4558 GGTCACC
BstPI 3500 GGTAACC G^GT[ACGT]ACC
4558 GGTCACC
CfoI 2720 GCGC GCG^C
5974 GCGC
DraII 3154 AGGACCT [GA]G^G[ACGT]CC[CT]
3320 GGGACCC
DsaI 726 CCACGG C^C[GA][CT]GG
1490 CCACGG
EcoO109I 3154 AGGACCT [GA]G^G[ACGT]CC[CT]
3320 GGGACCC
EcoO65I 3500 GGTAACC G^GT[ACGT]ACC
4558 GGTCACC
EcoT22I 1975 ATGCAT ATGCA^T
2129 ATGCAT
FbaI 328 TGATCA T^GATCA
4279 TGATCA
HaeII 2719 AGCGCC [GA]GCGC^[CT]
5973 AGCGCC
HhaI 2720 GCGC GCG^C
5974 GCGC
HinP1I 2720 GCGC G^CGC
5974 GCGC
HincII 1682 GTCAAC GT[CT]^[GA]AC
3785 GTTAAC
HindII 1682 GTCAAC GT[CT]^[GA]AC
3785 GTTAAC
MroI 1563 TCCGGA T^CCGGA
3667 TCCGGA
NsiI 1975 ATGCAT ATGCA^T
2129 ATGCAT
NspV 1806 TTCGAA TT^CGAA
3296 TTCGAA
PpuMI 3154 AGGACCT [GA]G^G[AT]CC[CT]
3320 GGGACCC
PvuII 27 CAGCTG CAG^CTG
183 CAGCTG
SfuI 1806 TTCGAA TT^CGAA
3296 TTCGAA
SspBI 2885 TGTACA T^GTACA
5440 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraIII EaeI EarI
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI