##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
Aor51HI AscI AspEI AviII AvrII
BcgI BclI BlnI BsiEI BsiWI
BspDI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I FbaI
FseI FspI KspI MluI NheI
NotI NruI NspV PacI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SnaBI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 323 TCCGGA T^CCGGA
ApaI 5854 GGGCCC GGGCC^C
AseI 3090 ATTAAT AT^TAAT
AsnI 3090 ATTAAT AT^TAAT
AspI 2963 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 61 GGCGCC GGCGC^C
BbrPI 1384 CACGTG CAC^GTG
BciVI 5373 GTATCC GTATCC(6/5)
BglI 423 GCCTGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1384 CACGTG [CT]AC^GT[GA]
BseAI 323 TCCGGA T^CCGGA
Bsp1407I 4330 TGTACA T^GTACA
BspEI 323 TCCGGA T^CCGGA
BspLU11I 6104 ACATGT A^CATGT
BsrBI 13 GAGCGG CCGCTC(-3/-3)
BsrGI 4330 TGTACA T^GTACA
DraI 5447 TTTAAA TTT^AAA
EcoRV 6822 GATATC GAT^ATC
HaeII 61 GGCGCC [GA]GCGC^[CT]
HgaI 508 GACGC GACGC(5/10)
KasI 61 GGCGCC G^GCGCC
MroI 323 TCCGGA T^CCGGA
NaeI 76 GCCGGC GCC^GGC
NarI 61 GGCGCC GG^CGCC
NdeI 4969 CATATG CA^TATG
NgoMIV 76 GCCGGC G^CCGGC
PflFI 2963 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1384 CACGTG CAC^GTG
PmlI 1384 CACGTG CAC^GTG
PshAI 4967 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3090 ATTAAT AT^TAAT
SacI 2413 GAGCTC GAGCT^C
SfoI 61 GGCGCC GGC^GCC
SgrAI 75 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2582 CCCGGG CCC^GGG
SpeI 6315 ACTAGT A^CTAGT
SspBI 4330 TGTACA T^GTACA
Tth111I 2963 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2582 CCCGGG C^CCGGG
XmaI 2582 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 5144 GGTACC G^GTACC
6117 GGTACC
AccII 100 CGCG CG^CG
815 CGCG
AcyI 61 GGCGCC G[GA]^CG[CT]C
508 GACGCC
Alw44I 2998 GTGCAC G^TGCAC
4078 GTGCAC
AlwNI 188 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
3027 CAGAGTCTG
ApaLI 2998 GTGCAC G^TGCAC
4078 GTGCAC
Asp718I 5144 GGTACC G^GTACC
6117 GGTACC
BglII 2388 AGATCT A^GATCT
4319 AGATCT
BsaHI 61 GGCGCC G[GA]^CG[CT]C
508 GACGCC
BsmBI 1159 GAGACG CGTCTC(1/5)
1398 GAGACG
BsrFI 76 GCCGGC [GA]^CCGG[CT]
1627 GCCGGT
BssSI 805 CACGAG CACGAG(-5/-1)
1395 CACGAG
BstEII 1255 GGTCACC G^GT[ACGT]ACC
5619 GGTCACC
BstPI 1255 GGTCACC G^GT[ACGT]ACC
5619 GGTCACC
BstUI 100 CGCG CG^CG
815 CGCG
BstXI 944 CCAGTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5822 CCAAGTAGCTGG
Cfr10I 76 GCCGGC [GA]^CCGG[CT]
1627 GCCGGT
EcoO65I 1255 GGTCACC G^GT[ACGT]ACC
5619 GGTCACC
EcoRI 2863 GAATTC G^AATTC
6653 GAATTC
Hin1I 61 GGCGCC G[GA]^CG[CT]C
508 GACGCC
HincII 1771 GTTAAC GT[CT]^[GA]AC
3038 GTTAAC
HindII 1771 GTTAAC GT[CT]^[GA]AC
3038 GTTAAC
HindIII 663 AAGCTT A^AGCTT
2126 AAGCTT
HpaI 1771 GTTAAC GTT^AAC
3038 GTTAAC
KpnI 5144 GGTACC GGTAC^C
6117 GGTACC
MfeI 4670 CAATTG C^AATTG
6619 CAATTG
MunI 4670 CAATTG C^AATTG
6619 CAATTG
MvnI 100 CGCG CG^CG
815 CGCG
NcoI 485 CCATGG C^CATGG
6363 CCATGG
PaeR7I 306 CTCGAG C^TCGAG
4745 CTCGAG
SapI 2593 GCTCTTC GCTCTTC(1/4)
6321 GCTCTTC
StuI 312 AGGCCT AGG^CCT
1601 AGGCCT
XhoI 306 CTCGAG C^TCGAG
4745 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AocI ApoI Asp700I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BfrI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SphI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI