##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AgeI Aor51HI
AscI Asp718I AviII BamHI BanI
BbeI BbrPI BclI BsaWI BseAI
BsiEI BspDI BspEI BsrBI BsrFI
BssHII Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI DraIII DsaI EagI
EclXI Eco47III Eco52I EcoRV FbaI
FseI FspI HaeII KasI KpnI
KspI MluI MroI NaeI NarI
NcoI NgoMIV NotI NspV PaeR7I
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 1064 AACGTT AA^CGTT
AcyI 3988 GACGCC G[GA]^CG[CT]C
AflII 6548 CTTAAG C^TTAAG
AhdI 3058 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 6417 GGGCCC GGGCC^C
Asp700I 3245 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3058 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 4474 CGATTCTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 6548 CTTAAG C^TTAAG
BglI 1902 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2491 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2491 GCTCAGC GC^T[ACGT]AGC
BsaAI 3415 TACGTG [CT]AC^GT[GA]
BsaBI 615 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3988 GACGCC G[GA]^CG[CT]C
BsiWI 5606 CGTACG C^GTACG
BspHI 1362 TCATGA T^CATGA
BsrDI 5292 CATTGC GCAATG(2/0)
BstAPI 1890 GCAACCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2491 GCTCAGC GC^T[ACGT]AGC
DrdI 795 GACAGTAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3058 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 3988 GACGCC G[GA]^CG[CT]C
HincII 2210 GTTAAC GT[CT]^[GA]AC
HindII 2210 GTTAAC GT[CT]^[GA]AC
HindIII 5037 AAGCTT A^AGCTT
HpaI 2210 GTTAAC GTT^AAC
MamI 615 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2147 CAATTG C^AATTG
MunI 2147 CAATTG C^AATTG
NheI 1183 GCTAGC G^CTAGC
NruI 5547 TCGCGA TCG^CGA
PacI 6017 TTAATTAA TTAAT^TAA
PpuMI 2902 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1064 AACGTT AA^CGTT
PvuII 473 CAGCTG CAG^CTG
RcaI 1362 TCATGA T^CATGA
SmaI 3936 CCCGGG CCC^GGG
SpeI 5202 ACTAGT A^CTAGT
XcmI 4578 CCAGGCTGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3936 CCCGGG C^CCGGG
XmaI 3936 CCCGGG C^CCGGG
XmnI 3245 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 2877 GTAGAC GT^[AC][GT]AC
3227 GTCTAC
AccII 4134 CGCG CG^CG
5548 CGCG
AflIII 756 ACATGT A^C[GA][CT]GT
3601 ACATGT
AlwNI 2940 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
6150 CAGCAACTG
AspI 2337 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3866 GACGGGGTC
AvaI 3936 CCCGGG C^[CT]CG[GA]G
4494 CCCGAG
AvrII 1666 CCTAGG C^CTAGG
3340 CCTAGG
BciVI 3756 GGATAC GTATCC(6/5)
2685 GTATCC
BlnI 1666 CCTAGG C^CTAGG
3340 CCTAGG
BsmBI 3864 GAGACG CGTCTC(1/5)
4031 GAGACG
BsoBI 3936 CCCGGG C^[CT]CG[GA]G
4494 CCCGAG
Bsp1407I 1291 TGTACA T^GTACA
6114 TGTACA
BspLU11I 756 ACATGT A^CATGT
3601 ACATGT
BsrGI 1291 TGTACA T^GTACA
6114 TGTACA
BstEII 1107 GGTTACC G^GT[ACGT]ACC
2772 GGTTACC
BstPI 1107 GGTTACC G^GT[ACGT]ACC
2772 GGTTACC
BstUI 4134 CGCG CG^CG
5548 CGCG
EcoNI 1872 CCTTATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2449 CCTCTTTGAGG
EcoO65I 1107 GGTTACC G^GT[ACGT]ACC
2772 GGTTACC
FauI 4525 CCCGC CCCGC(4/6)
3303 GCGGG
HgaI 3988 GACGC GACGC(5/10)
802 GCGTC
MspA1I 473 CAGCTG C[AC]G^C[GT]G
806 CCGCTG
MvnI 4134 CGCG CG^CG
5548 CGCG
PflFI 2337 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3866 GACGGGGTC
ScaI 2407 AGTACT AGT^ACT
3803 AGTACT
SphI 2245 GCATGC GCATG^C
6451 GCATGC
SspBI 1291 TGTACA T^GTACA
6114 TGTACA
SspI 2654 AATATT AAT^ATT
6529 AATATT
StuI 2667 AGGCCT AGG^CCT
3271 AGGCCT
TaqI 968 TCGA T^CGA
4775 TCGA
Tth111I 2337 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3866 GACGGGGTC
TthHB8I 968 TCGA T^CGA
4775 TCGA
XbaI 4197 TCTAGA T^CTAGA
6254 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AocI ApaLI ApoI AseI
AsnI AspHI AvaII BalI BanII
BbsI BbvCI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BssSI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PshAI PshBI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII