################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## Acc65I AccI AclI AflII AgeI AscI Asp718I AviII AvrII BbrPI BclI BfrI BglI BlnI BsaAI BsgI BsiEI BsiWI BspDI BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI DraIII DrdI EagI EclXI Eco52I EcoNI EcoO65I FbaI FseI FspI KpnI NaeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 1811 GACGTC GACGT^C AccIII 2139 TCCGGA T^CCGGA AhdI 526 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2665 GTGCAC G^TGCAC Aor51HI 200 AGCGCT AGC^GCT ApaI 4992 GGGCCC GGGCC^C ApaLI 2665 GTGCAC G^TGCAC AspEI 526 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 6464 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1801 TGGCCA TGG^CCA BbeI 6338 GGCGCC GGCGC^C BcgI 5618 GCACCTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3659 GGATAC GTATCC(6/5) BlpI 2171 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2171 GCTCAGC GC^T[ACGT]AGC BsaWI 2139 TCCGGA [AT]^CCGG[AT] BseAI 2139 TCCGGA T^CCGGA BspEI 2139 TCCGGA T^CCGGA BsrDI 3000 CATTGC GCAATG(2/0) BstAPI 2088 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 2171 GCTCAGC GC^T[ACGT]AGC CpoI 2457 CGGTCCG CG^G[AT]CCG Eam1105I 526 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 200 AGCGCT AGC^GCT EcoRI 2600 GAATTC G^AATTC HincII 5774 GTTAAC GT[CT]^[GA]AC HindII 5774 GTTAAC GT[CT]^[GA]AC HindIII 2687 AAGCTT A^AGCTT HpaI 5774 GTTAAC GTT^AAC KasI 6338 GGCGCC G^GCGCC KspI 474 CCGCGG CCGC^GG MfeI 4025 CAATTG C^AATTG MluI 352 ACGCGT A^CGCGT MluNI 1801 TGGCCA TGG^CCA MroI 2139 TCCGGA T^CCGGA MscI 1801 TGGCCA TGG^CCA MunI 4025 CAATTG C^AATTG NarI 6338 GGCGCC GG^CGCC NcoI 2588 CCATGG C^CATGG PaeR7I 2061 CTCGAG C^TCGAG PflFI 6464 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 4816 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 6429 CTGCAG CTGCA^G RsrII 2457 CGGTCCG CG^G[AT]CCG SacII 474 CCGCGG CCGC^GG SfoI 6338 GGCGCC GGC^GCC SmaI 6390 CCCGGG CCC^GGG SphI 1480 GCATGC GCATG^C StuI 1598 AGGCCT AGG^CCT Tth111I 6464 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2061 CTCGAG C^TCGAG XmaCI 6390 CCCGGG C^CCGGG XmaI 6390 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 1811 GACGTC G[GA]^CG[CT]C 6338 GGCGCC AocI 2389 CCTAAGG CC^T[ACGT]AGG 2726 CCTCAGG AseI 4191 ATTAAT AT^TAAT 5376 ATTAAT AsnI 4191 ATTAAT AT^TAAT 5376 ATTAAT BamHI 922 GGATCC G^GATCC 1924 GGATCC BanI 5617 GGCACC G^G[CT][GA]CC 6338 GGCGCC BglII 950 AGATCT A^GATCT 4355 AGATCT BsaBI 656 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4188 GATATTAATC BsaHI 1811 GACGTC G[GA]^CG[CT]C 6338 GGCGCC Bsp1407I 2308 TGTACA T^GTACA 5053 TGTACA BspHI 3958 TCATGA T^CATGA 6247 TCATGA BspMI 1714 ACCTGC ACCTGC(4/8) 3311 GCAGGT BsrFI 181 ACCGGC [GA]^CCGG[CT] 5633 GCCGGT BsrGI 2308 TGTACA T^GTACA 5053 TGTACA BstXI 6217 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6351 CCAGCTACTTGG Bsu36I 2389 CCTAAGG CC^T[ACGT]AGG 2726 CCTCAGG Cfr10I 181 ACCGGC [GA]^CCGG[CT] 5633 GCCGGT Eco81I 2389 CCTAAGG CC^T[ACGT]AGG 2726 CCTCAGG EcoT22I 778 ATGCAT ATGCA^T 2997 ATGCAT HaeII 200 AGCGCT [GA]GCGC^[CT] 6338 GGCGCC HgaI 1061 GACGC GACGC(5/10) 1123 GCGTC Hin1I 1811 GACGTC G[GA]^CG[CT]C 6338 GGCGCC MamI 656 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4188 GATATTAATC NdeI 1533 CATATG CA^TATG 5049 CATATG NsiI 778 ATGCAT ATGCA^T 2997 ATGCAT PshBI 4191 ATTAAT AT^TAAT 5376 ATTAAT RcaI 3958 TCATGA T^CATGA 6247 TCATGA SacI 581 GAGCTC GAGCT^C 5919 GAGCTC SspBI 2308 TGTACA T^GTACA 5053 TGTACA XcmI 2772 CCAGGCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4418 CCAAGAATCCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII XmnI