##################################
# NO CUT ENZYMES (   62 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
AscI        Asp718I     AviII       AvrII       BbrPI       
BclI        BfrI        BglI        BlnI        BsaAI       
BsgI        BsiEI       BsiWI       BspDI       BsrBI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        DraIII      DrdI        
EagI        EclXI       Eco52I      EcoNI       EcoO65I     
FbaI        FseI        FspI        KpnI        NaeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PvuI        SalI        SbfI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII        1811  GACGTC               GACGT^C
AccIII       2139  TCCGGA               T^CCGGA
AhdI          526  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2665  GTGCAC               G^TGCAC
Aor51HI       200  AGCGCT               AGC^GCT
ApaI         4992  GGGCCC               GGGCC^C
ApaLI        2665  GTGCAC               G^TGCAC
AspEI         526  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         6464  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1801  TGGCCA               TGG^CCA
BbeI         6338  GGCGCC               GGCGC^C
BcgI         5618  GCACCTCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3659  GGATAC               GTATCC(6/5)
BlpI         2171  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2171  GCTCAGC              GC^T[ACGT]AGC
BsaWI        2139  TCCGGA               [AT]^CCGG[AT]
BseAI        2139  TCCGGA               T^CCGGA
BspEI        2139  TCCGGA               T^CCGGA
BsrDI        3000  CATTGC               GCAATG(2/0)
BstAPI       2088  GCATATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        2171  GCTCAGC              GC^T[ACGT]AGC
CpoI         2457  CGGTCCG              CG^G[AT]CCG
Eam1105I      526  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      200  AGCGCT               AGC^GCT
EcoRI        2600  GAATTC               G^AATTC
HincII       5774  GTTAAC               GT[CT]^[GA]AC
HindII       5774  GTTAAC               GT[CT]^[GA]AC
HindIII      2687  AAGCTT               A^AGCTT
HpaI         5774  GTTAAC               GTT^AAC
KasI         6338  GGCGCC               G^GCGCC
KspI          474  CCGCGG               CCGC^GG
MfeI         4025  CAATTG               C^AATTG
MluI          352  ACGCGT               A^CGCGT
MluNI        1801  TGGCCA               TGG^CCA
MroI         2139  TCCGGA               T^CCGGA
MscI         1801  TGGCCA               TGG^CCA
MunI         4025  CAATTG               C^AATTG
NarI         6338  GGCGCC               GG^CGCC
NcoI         2588  CCATGG               C^CATGG
PaeR7I       2061  CTCGAG               C^TCGAG
PflFI        6464  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        4816  GACACAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         6429  CTGCAG               CTGCA^G
RsrII        2457  CGGTCCG              CG^G[AT]CCG
SacII         474  CCGCGG               CCGC^GG
SfoI         6338  GGCGCC               GGC^GCC
SmaI         6390  CCCGGG               CCC^GGG
SphI         1480  GCATGC               GCATG^C
StuI         1598  AGGCCT               AGG^CCT
Tth111I      6464  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2061  CTCGAG               C^TCGAG
XmaCI        6390  CCCGGG               C^CCGGG
XmaI         6390  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcyI         1811  GACGTC               G[GA]^CG[CT]C
             6338  GGCGCC              
AocI         2389  CCTAAGG              CC^T[ACGT]AGG
             2726  CCTCAGG             
AseI         4191  ATTAAT               AT^TAAT
             5376  ATTAAT              
AsnI         4191  ATTAAT               AT^TAAT
             5376  ATTAAT              
BamHI         922  GGATCC               G^GATCC
             1924  GGATCC              
BanI         5617  GGCACC               G^G[CT][GA]CC
             6338  GGCGCC              
BglII         950  AGATCT               A^GATCT
             4355  AGATCT              
BsaBI         656  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4188  GATATTAATC          
BsaHI        1811  GACGTC               G[GA]^CG[CT]C
             6338  GGCGCC              
Bsp1407I     2308  TGTACA               T^GTACA
             5053  TGTACA              
BspHI        3958  TCATGA               T^CATGA
             6247  TCATGA              
BspMI        1714  ACCTGC               ACCTGC(4/8)
             3311  GCAGGT              
BsrFI         181  ACCGGC               [GA]^CCGG[CT]
             5633  GCCGGT              
BsrGI        2308  TGTACA               T^GTACA
             5053  TGTACA              
BstXI        6217  CCAGCACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             6351  CCAGCTACTTGG        
Bsu36I       2389  CCTAAGG              CC^T[ACGT]AGG
             2726  CCTCAGG             
Cfr10I        181  ACCGGC               [GA]^CCGG[CT]
             5633  GCCGGT              
Eco81I       2389  CCTAAGG              CC^T[ACGT]AGG
             2726  CCTCAGG             
EcoT22I       778  ATGCAT               ATGCA^T
             2997  ATGCAT              
HaeII         200  AGCGCT               [GA]GCGC^[CT]
             6338  GGCGCC              
HgaI         1061  GACGC                GACGC(5/10)
             1123  GCGTC               
Hin1I        1811  GACGTC               G[GA]^CG[CT]C
             6338  GGCGCC              
MamI          656  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4188  GATATTAATC          
NdeI         1533  CATATG               CA^TATG
             5049  CATATG              
NsiI          778  ATGCAT               ATGCA^T
             2997  ATGCAT              
PshBI        4191  ATTAAT               AT^TAAT
             5376  ATTAAT              
RcaI         3958  TCATGA               T^CATGA
             6247  TCATGA              
SacI          581  GAGCTC               GAGCT^C
             5919  GAGCTC              
SspBI        2308  TGTACA               T^GTACA
             5053  TGTACA              
XcmI         2772  CCAGGCACACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4418  CCAAGAATCCACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaI        AvaII       BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspLU11I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoRV       EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XbaI        XhoII       XmnI