##################################
# NO CUT ENZYMES ( 36 enzymes) #
##################################
AatII AclI AgeI AscI AviII
AvrII BbrPI BlnI BsiWI BssHII
Bst1107I BstZ17I CpoI DraIII FseI
FspI HpaI NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SalI
SbfI SexAI SgrAI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 6111 GGTACC G^GTACC
AccI 3161 GTCTAC GT^[AC][GT]AC
AccIII 5319 TCCGGA T^CCGGA
Alw44I 5782 GTGCAC G^TGCAC
Aor51HI 269 AGCGCT AGC^GCT
ApaLI 5782 GTGCAC G^TGCAC
Asp718I 6111 GGTACC G^GTACC
AspI 4619 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 437 GGATCC G^GATCC
BclI 3434 TGATCA T^GATCA
BsaBI 4121 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 5319 TCCGGA [AT]^CCGG[AT]
BseAI 5319 TCCGGA T^CCGGA
BsgI 4501 CTGCAC GTGCAG(16/14)
BsiEI 237 CGGCCG CG[GA][CT]^CG
BsmBI 6076 CGTCTC CGTCTC(1/5)
Bsp1407I 3650 TGTACA T^GTACA
BspDI 1039 ATCGAT AT^CGAT
BspEI 5319 TCCGGA T^CCGGA
BsrDI 1454 CATTGC GCAATG(2/0)
BsrGI 3650 TGTACA T^GTACA
BstBI 3038 TTCGAA TT^CGAA
BstEII 381 GGTAACC G^GT[ACGT]ACC
BstPI 381 GGTAACC G^GT[ACGT]ACC
ClaI 1039 ATCGAT AT^CGAT
EagI 237 CGGCCG C^GGCCG
EclXI 237 CGGCCG C^GGCCG
Eco47III 269 AGCGCT AGC^GCT
Eco52I 237 CGGCCG C^GGCCG
EcoO65I 381 GGTAACC G^GT[ACGT]ACC
EcoT22I 2997 ATGCAT ATGCA^T
FbaI 3434 TGATCA T^GATCA
KpnI 6111 GGTACC GGTAC^C
KspI 376 CCGCGG CCGC^GG
MamI 4121 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 288 ACGCGT A^CGCGT
MroI 5319 TCCGGA T^CCGGA
NdeI 4893 CATATG CA^TATG
NsiI 2997 ATGCAT ATGCA^T
NspV 3038 TTCGAA TT^CGAA
PaeR7I 785 CTCGAG C^TCGAG
PflFI 4619 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 376 CCGCGG CCGC^GG
ScaI 3054 AGTACT AGT^ACT
SfiI 1008 GGCCTCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3038 TTCGAA TT^CGAA
SspBI 3650 TGTACA T^GTACA
Tth111I 4619 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 785 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflII 3184 CTTAAG C^TTAAG
4409 CTTAAG
AhdI 5604 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6418 GACAGCTTGTC
ApaI 4357 GGGCCC GGGCC^C
5809 GGGCCC
AseI 2726 ATTAAT AT^TAAT
3834 ATTAAT
AsnI 2726 ATTAAT AT^TAAT
3834 ATTAAT
AspEI 5604 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6418 GACAGCTTGTC
BcgI 3040 CGAAGTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5791 CGAGGAGAATGC
BciVI 557 GGATAC GTATCC(6/5)
1539 GTATCC
BfrI 3184 CTTAAG C^TTAAG
4409 CTTAAG
BglI 1000 GCCGAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1009 GCCTCCACGGC
BsaAI 493 TACGTA [CT]AC^GT[GA]
3816 TACGTA
BspLU11I 3143 ACATGT A^CATGT
3341 ACATGT
BsrFI 34 GCCGGC [GA]^CCGG[CT]
147 GCCGGC
Cfr10I 34 GCCGGC [GA]^CCGG[CT]
147 GCCGGC
DrdI 1239 GACACCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3522 GACGTTGGGGTC
Eam1105I 5604 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6418 GACAGCTTGTC
EarI 4471 CTCTTC CTCTTC(1/4)
837 GAAGAG
HincII 2293 GTTGAC GT[CT]^[GA]AC
2843 GTTGAC
HindII 2293 GTTGAC GT[CT]^[GA]AC
2843 GTTGAC
Ksp632I 4471 CTCTTC CTCTTC(1/4)
837 GAAGAG
MfeI 2957 CAATTG C^AATTG
3211 CAATTG
MunI 2957 CAATTG C^AATTG
3211 CAATTG
NaeI 34 GCCGGC GCC^GGC
147 GCCGGC
NgoMIV 34 GCCGGC G^CCGGC
147 GCCGGC
PflMI 4605 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4998 CCAGTGTGTGG
PshBI 2726 ATTAAT AT^TAAT
3834 ATTAAT
PvuII 3659 CAGCTG CAG^CTG
6554 CAGCTG
SacI 2090 GAGCTC GAGCT^C
5532 GAGCTC
SapI 837 GAAGAGC GCTCTTC(1/4)
4470 GCTCTTC
SnaBI 493 TACGTA TAC^GTA
3816 TACGTA
SpeI 3785 ACTAGT A^CTAGT
4231 ACTAGT
SphI 4932 GCATGC GCATG^C
4960 GCATGC
Van91I 4605 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4998 CCAGTGTGTGG
XbaI 1104 TCTAGA T^CTAGA
2317 TCTAGA
XcmI 521 CCAACAGCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2068 CCACATCTTTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI XmnI