##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
Alw44I Aor51HI ApaLI AscI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BlnI BsaHI BsaWI
BseAI BsiEI BsmBI BspDI BspEI
BsrBI BssHII BstEII BstPI BstUI
ClaI CpoI DraIII DsaI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FseI FspI Hin1I
KasI KspI MluI MluNI MroI
MscI MvnI NarI NcoI NotI
NruI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacI SacII SalI SbfI
SfiI SfoI SgrAI SnaBI Sse8387I
StuI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AhdI 4231 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 5832 GGGCCC GGGCC^C
AspEI 4231 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3785 GAGCAC G[AT]GC[AT]^C
AspI 5510 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 5437 GCTGAGG CCTCAGC(-5/-2)
BclI 3670 TGATCA T^GATCA
BglI 2870 GCCGTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4891 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4891 GCTCAGC GC^T[ACGT]AGC
BsaAI 5061 CACGTA [CT]AC^GT[GA]
BsaBI 4965 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 3785 GAGCAC G[AT]GC[AT]^C
BsiWI 3818 CGTACG C^GTACG
BsmFI 81 GGGAC GGGAC(10/14)
Bsp1407I 5124 TGTACA T^GTACA
BspMI 1175 GCAGGT ACCTGC(4/8)
BsrFI 15 GCCGGC [GA]^CCGG[CT]
BsrGI 5124 TGTACA T^GTACA
BssSI 3782 CACGAG CACGAG(-5/-1)
BstAPI 4700 GCAACCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 4891 GCTCAGC GC^T[ACGT]AGC
CfoI 3987 GCGC GCG^C
Cfr10I 15 GCCGGC [GA]^CCGG[CT]
Eam1105I 4231 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3670 TGATCA T^GATCA
HaeII 3986 GGCGCT [GA]GCGC^[CT]
HgaI 566 GACGC GACGC(5/10)
HhaI 3987 GCGC GCG^C
HinP1I 3987 GCGC G^CGC
HincII 3475 GTTAAC GT[CT]^[GA]AC
HindII 3475 GTTAAC GT[CT]^[GA]AC
HpaI 3475 GTTAAC GTT^AAC
MamI 4965 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3617 CAATTG C^AATTG
MunI 3617 CAATTG C^AATTG
NaeI 15 GCCGGC GCC^GGC
NdeI 6144 CATATG CA^TATG
NgoMIV 15 GCCGGC G^CCGGC
NheI 1171 GCTAGC G^CTAGC
PflFI 5510 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 5314 AGTACT AGT^ACT
SexAI 4840 ACCAGGT A^CC[AT]GGT
SmaI 4482 CCCGGG CCC^GGG
SpeI 6439 ACTAGT A^CTAGT
SspBI 5124 TGTACA T^GTACA
Tth111I 5510 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 4482 CCCGGG C^CCGGG
XmaI 4482 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 1609 GGTACC G^GTACC
5347 GGTACC
AclI 3447 AACGTT AA^CGTT
5548 AACGTT
AflIII 2612 ACATGT A^C[GA][CT]GT
5655 ACATGT
AlwNI 207 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
3663 CAGAAACTG
AocI 5289 CCTCAGG CC^T[ACGT]AGG
6107 CCTTAGG
Asp718I 1609 GGTACC G^GTACC
5347 GGTACC
AvaI 4482 CCCGGG C^[CT]CG[GA]G
5493 CTCGGG
BanI 1609 GGTACC G^G[CT][GA]CC
5347 GGTACC
BanII 5790 GAGCCC G[GA]GC[CT]^C
5832 GGGCCC
BglII 2980 AGATCT A^GATCT
3157 AGATCT
BsaI 2909 GAGACC GGTCTC(1/5)
5508 GAGACC
BsgI 5482 CTGCAC GTGCAG(16/14)
4662 GTGCAG
BsmI 2545 GAATGC GAATGC(1/-1)
2825 GAATGC
BsoBI 4482 CCCGGG C^[CT]CG[GA]G
5493 CTCGGG
BspHI 1081 TCATGA T^CATGA
1498 TCATGA
BspLU11I 2612 ACATGT A^CATGT
5655 ACATGT
Bst1107I 1658 GTATAC GTA^TAC
5759 GTATAC
BstBI 1463 TTCGAA TT^CGAA
4266 TTCGAA
BstZ17I 1658 GTATAC GTA^TAC
5759 GTATAC
Bsu36I 5289 CCTCAGG CC^T[ACGT]AGG
6107 CCTTAGG
DrdI 1276 GACATTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3178 GACATTACAGTC
Eco81I 5289 CCTCAGG CC^T[ACGT]AGG
6107 CCTTAGG
EcoRI 3905 GAATTC G^AATTC
5264 GAATTC
EcoT14I 2316 CCTTGG C^C[AT][AT]GG
3876 CCAAGG
EcoT22I 5232 ATGCAT ATGCA^T
6461 ATGCAT
KpnI 1609 GGTACC GGTAC^C
5347 GGTACC
NsiI 5232 ATGCAT ATGCA^T
6461 ATGCAT
NspV 1463 TTCGAA TT^CGAA
4266 TTCGAA
PpuMI 4260 AGGTCCT [GA]G^G[AT]CC[CT]
5293 AGGACCC
Psp1406I 3447 AACGTT AA^CGTT
5548 AACGTT
RcaI 1081 TCATGA T^CATGA
1498 TCATGA
SfuI 1463 TTCGAA TT^CGAA
4266 TTCGAA
StyI 2316 CCTTGG C^C[AT][AT]GG
3876 CCAAGG
SwaI 2169 ATTTAAAT ATTT^AAAT
6557 ATTTAAAT
XbaI 2533 TCTAGA T^CTAGA
4462 TCTAGA
XcmI 3633 CCAAAAAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4620 CCAAAGAAGAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI AseI AsnI
Asp700I AvaII BbsI BbvI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PshBI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI