##################################
# NO CUT ENZYMES ( 31 enzymes) #
##################################
AatII Acc65I AclI AscI Asp718I
AviII BbeI BsiWI BssHII Bst1107I
BstZ17I CpoI DraIII FseI FspI
KasI KpnI KspI MluI NarI
NotI NruI PacI PshAI Psp1406I
RsrII SacII SfiI SfoI SgrAI
XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 4194 TCCGGA T^CCGGA
AcyI 1632 GGCGTC G[GA]^CG[CT]C
AgeI 12582 ACCGGT A^CCGGT
Alw44I 11204 GTGCAC G^TGCAC
ApaLI 11204 GTGCAC G^TGCAC
AvrII 11038 CCTAGG C^CTAGG
BlnI 11038 CCTAGG C^CTAGG
BsaHI 1632 GGCGTC G[GA]^CG[CT]C
BseAI 4194 TCCGGA T^CCGGA
BspEI 4194 TCCGGA T^CCGGA
BstBI 3803 TTCGAA TT^CGAA
EagI 4904 CGGCCG C^GGCCG
EclXI 4904 CGGCCG C^GGCCG
Eco52I 4904 CGGCCG C^GGCCG
EcoRV 10873 GATATC GAT^ATC
Hin1I 1632 GGCGTC G[GA]^CG[CT]C
MroI 4194 TCCGGA T^CCGGA
NheI 2543 GCTAGC G^CTAGC
NspV 3803 TTCGAA TT^CGAA
PinAI 12582 ACCGGT A^CCGGT
PmeI 10527 GTTTAAAC GTTT^AAAC
PvuI 5497 CGATCG CGAT^CG
SbfI 3229 CCTGCAGG CCTGCA^GG
SfuI 3803 TTCGAA TT^CGAA
SmaI 12813 CCCGGG CCC^GGG
SnaBI 888 TACGTA TAC^GTA
SpeI 8095 ACTAGT A^CTAGT
Sse8387I 3229 CCTGCAGG CCTGCA^GG
SwaI 14141 ATTTAAAT ATTT^AAAT
XmaCI 12813 CCCGGG C^CCGGG
XmaI 12813 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflII 1561 CTTAAG C^TTAAG
10039 CTTAAG
Aor51HI 1128 AGCGCT AGC^GCT
13187 AGCGCT
AseI 7036 ATTAAT AT^TAAT
13596 ATTAAT
AsnI 7036 ATTAAT AT^TAAT
13596 ATTAAT
BciVI 11062 GGATAC GTATCC(6/5)
1215 GTATCC
BfrI 1561 CTTAAG C^TTAAG
10039 CTTAAG
BglI 2115 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4670 GCCTTCCTGGC
BsaWI 4194 TCCGGA [AT]^CCGG[AT]
12582 ACCGGT
BsmBI 9695 GAGACG CGTCTC(1/5)
13151 GAGACG
BsmI 1933 GAATGC GAATGC(1/-1)
4975 GAATGC
BspDI 2453 ATCGAT AT^CGAT
12335 ATCGAT
ClaI 2453 ATCGAT AT^CGAT
12335 ATCGAT
DrdI 9899 GACCCAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
12215 GACTTTGATGTC
Eco47III 1128 AGCGCT AGC^GCT
13187 AGCGCT
NaeI 11421 GCCGGC GCC^GGC
13113 GCCGGC
NdeI 6704 CATATG CA^TATG
10259 CATATG
NgoMIV 11421 GCCGGC G^CCGGC
13113 GCCGGC
PaeR7I 1187 CTCGAG C^TCGAG
12267 CTCGAG
PshBI 7036 ATTAAT AT^TAAT
13596 ATTAAT
SalI 991 GTCGAC G^TCGAC
6699 GTCGAC
SexAI 4038 ACCTGGT A^CC[AT]GGT
5570 ACCTGGT
SphI 1518 GCATGC GCATG^C
9823 GCATGC
XhoI 1187 CTCGAG C^TCGAG
12267 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 173 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspEI
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbrPI BbsI
BbvCI BbvI BcgI BclI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmnI