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# NO CUT ENZYMES ( 28 enzymes) #
##################################
AclI AgeI AscI AseI AsnI
BclI BsiWI BspDI Bst1107I BstZ17I
ClaI DrdI EcoRV FbaI MfeI
MunI NheI NotI PacI PinAI
PmeI PshBI Psp1406I PvuI SnaBI
SpeI SwaI XbaI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 4273 GGTACC G^GTACC
AflII 3603 CTTAAG C^TTAAG
Asp718I 4273 GGTACC G^GTACC
AspI 3894 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 5834 GGATCC G^GATCC
BciVI 10356 GGATAC GTATCC(6/5)
BfrI 3603 CTTAAG C^TTAAG
BsaBI 4977 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 10403 TGTACA T^GTACA
BspHI 3998 TCATGA T^CATGA
BsrGI 10403 TGTACA T^GTACA
BstBI 5845 TTCGAA TT^CGAA
CpoI 7805 CGGACCG CG^G[AT]CCG
DraI 10422 TTTAAA TTT^AAA
EcoRI 2500 GAATTC G^AATTC
EcoT22I 10200 ATGCAT ATGCA^T
FseI 309 GGCCGGCC GGCCGG^CC
HpaI 2568 GTTAAC GTT^AAC
KpnI 4273 GGTACC GGTAC^C
MamI 4977 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 6713 ACGCGT A^CGCGT
NdeI 1634 CATATG CA^TATG
NruI 4821 TCGCGA TCG^CGA
NsiI 10200 ATGCAT ATGCA^T
NspV 5845 TTCGAA TT^CGAA
PflFI 3894 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 3998 TCATGA T^CATGA
RsrII 7805 CGGACCG CG^G[AT]CCG
ScaI 6432 AGTACT AGT^ACT
SfiI 5715 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5845 TTCGAA TT^CGAA
SgrAI 6204 CACCGGCG C[GA]^CCGG[CT]G
SphI 2958 GCATGC GCATG^C
SspBI 10403 TGTACA T^GTACA
SspI 9648 AATATT AAT^ATT
Tth111I 3894 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 4037 TCCGGA T^CCGGA
4490 TCCGGA
AhdI 1534 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8851 GACCTGGAGTC
Asp700I 3988 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
10367 GAATGGCTTC
AspEI 1534 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8851 GACCTGGAGTC
AvrII 217 CCTAGG C^CTAGG
8892 CCTAGG
BalI 207 TGGCCA TGG^CCA
9804 TGGCCA
BcgI 2391 GCATGAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3386 GCACAGAGCTCG
BglII 4305 AGATCT A^GATCT
7707 AGATCT
BlnI 217 CCTAGG C^CTAGG
8892 CCTAGG
BseAI 4037 TCCGGA T^CCGGA
4490 TCCGGA
BsmI 366 GCATTC GAATGC(1/-1)
8718 GCATTC
BspEI 4037 TCCGGA T^CCGGA
4490 TCCGGA
BspLU11I 6652 ACATGT A^CATGT
6830 ACATGT
BstXI 2273 CCAGAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
8321 CCAAGGCCCTGG
Eam1105I 1534 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8851 GACCTGGAGTC
HindIII 3990 AAGCTT A^AGCTT
9256 AAGCTT
KspI 31 CCGCGG CCGC^GG
298 CCGCGG
MluNI 207 TGGCCA TGG^CCA
9804 TGGCCA
MroI 4037 TCCGGA T^CCGGA
4490 TCCGGA
MscI 207 TGGCCA TGG^CCA
9804 TGGCCA
NcoI 6566 CCATGG C^CATGG
7572 CCATGG
PshAI 3158 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5337 GACTGCTGTC
SacII 31 CCGCGG CCGC^GG
298 CCGCGG
SalI 3870 GTCGAC G^TCGAC
8375 GTCGAC
SbfI 2937 CCTGCAGG CCTGCA^GG
7770 CCTGCAGG
SexAI 3590 ACCAGGT A^CC[AT]GGT
9613 ACCAGGT
Sse8387I 2937 CCTGCAGG CCTGCA^GG
7770 CCTGCAGG
XmnI 3988 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
10367 GAATGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 166 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AspHI AvaI AvaII
AviII BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PaeR7I PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI