##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AgeI        AhdI        Aor51HI     AscI        Asp718I     
AspEI       AviII       AvrII       BbeI        BcgI        
BlnI        BsaBI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRI       
EcoRV       EcoT22I     FseI        FspI        HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PinAI       PmeI        PshAI       Psp1406I    
PvuI        RsrII       SacII       SalI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII        2517  CGCG                 CG^CG
AcyI         2722  GACGCC               G[GA]^CG[CT]C
AflII        1170  CTTAAG               C^TTAAG
Alw44I        549  GTGCAC               G^TGCAC
ApaI         4766  GGGCCC               GGGCC^C
ApaLI         549  GTGCAC               G^TGCAC
AseI         4509  ATTAAT               AT^TAAT
AsnI         4509  ATTAAT               AT^TAAT
BamHI        2435  GGATCC               G^GATCC
BclI         3757  TGATCA               T^GATCA
BfrI         1170  CTTAAG               C^TTAAG
BsaHI        2722  GACGCC               G[GA]^CG[CT]C
BsaWI         312  TCCGGT               [AT]^CCGG[AT]
Bsp1407I       55  TGTACA               T^GTACA
BspHI        1879  TCATGA               T^CATGA
BspLU11I     4014  ACATGT               A^CATGT
BsrBI         764  GAGCGG               CCGCTC(-3/-3)
BsrFI         192  ACCGGC               [GA]^CCGG[CT]
BsrGI          55  TGTACA               T^GTACA
BstEII       4025  GGTAACC              G^GT[ACGT]ACC
BstPI        4025  GGTAACC              G^GT[ACGT]ACC
BstUI        2517  CGCG                 CG^CG
Cfr10I        192  ACCGGC               [GA]^CCGG[CT]
DrdI         4290  GACCTGGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      4025  GGTAACC              G^GT[ACGT]ACC
FbaI         3757  TGATCA               T^GATCA
Hin1I        2722  GACGCC               G[GA]^CG[CT]C
HincII        623  GTTGAC               GT[CT]^[GA]AC
HindII        623  GTTGAC               GT[CT]^[GA]AC
HindIII      3655  AAGCTT               A^AGCTT
MluI         2516  ACGCGT               A^CGCGT
MvnI         2517  CGCG                 CG^CG
NheI         3910  GCTAGC               G^CTAGC
PshBI        4509  ATTAAT               AT^TAAT
RcaI         1879  TCATGA               T^CATGA
SbfI         2631  CCTGCAGG             CCTGCA^GG
SexAI        2239  ACCTGGT              A^CC[AT]GGT
SmaI          629  CCCGGG               CCC^GGG
SphI         4690  GCATGC               GCATG^C
Sse8387I     2631  CCTGCAGG             CCTGCA^GG
SspBI          55  TGTACA               T^GTACA
SspI         1552  AATATT               AAT^ATT
XmaCI         629  CCCGGG               C^CCGGG
XmaI          629  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

Asp700I      1518  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4078  GAAAGATTTC          
BbrPI         461  CACGTG               CAC^GTG
             2526  CACGTG              
BciVI         423  GTATCC               GTATCC(6/5)
             2520  GTATCC              
BglI          518  GCCCTGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1626  GCCTGCTAGGC         
BglII         434  AGATCT               A^GATCT
             4307  AGATCT              
BsmBI        2448  CGTCTC               CGTCTC(1/5)
              664  GAGACG              
BsmI         1758  GAATGC               GAATGC(1/-1)
             4411  GCATTC              
NcoI         2840  CCATGG               C^CATGG
             4760  CCATGG              
PaeR7I       2923  CTCGAG               C^TCGAG
             3093  CTCGAG              
PmaCI         461  CACGTG               CAC^GTG
             2526  CACGTG              
PmlI          461  CACGTG               CAC^GTG
             2526  CACGTG              
PvuII        2548  CAGCTG               CAG^CTG
             4827  CAGCTG              
StuI         1745  AGGCCT               AGG^CCT
             4242  AGGCCT              
XhoI         2923  CTCGAG               C^TCGAG
             3093  CTCGAG              
XmnI         1518  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4078  GAAAGATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       AocI        ApoI        AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflFI       
PflMI       PleI        PpuMI       PstI        RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII