################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI AhdI Aor51HI AscI Asp718I AspEI AviII AvrII BbeI BcgI BlnI BsaBI BseAI BsiEI BsiWI BspDI BspEI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HpaI KasI KpnI KspI MamI MfeI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SwaI XbaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 2517 CGCG CG^CG AcyI 2722 GACGCC G[GA]^CG[CT]C AflII 1170 CTTAAG C^TTAAG Alw44I 549 GTGCAC G^TGCAC ApaI 4766 GGGCCC GGGCC^C ApaLI 549 GTGCAC G^TGCAC AseI 4509 ATTAAT AT^TAAT AsnI 4509 ATTAAT AT^TAAT BamHI 2435 GGATCC G^GATCC BclI 3757 TGATCA T^GATCA BfrI 1170 CTTAAG C^TTAAG BsaHI 2722 GACGCC G[GA]^CG[CT]C BsaWI 312 TCCGGT [AT]^CCGG[AT] Bsp1407I 55 TGTACA T^GTACA BspHI 1879 TCATGA T^CATGA BspLU11I 4014 ACATGT A^CATGT BsrBI 764 GAGCGG CCGCTC(-3/-3) BsrFI 192 ACCGGC [GA]^CCGG[CT] BsrGI 55 TGTACA T^GTACA BstEII 4025 GGTAACC G^GT[ACGT]ACC BstPI 4025 GGTAACC G^GT[ACGT]ACC BstUI 2517 CGCG CG^CG Cfr10I 192 ACCGGC [GA]^CCGG[CT] DrdI 4290 GACCTGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 4025 GGTAACC G^GT[ACGT]ACC FbaI 3757 TGATCA T^GATCA Hin1I 2722 GACGCC G[GA]^CG[CT]C HincII 623 GTTGAC GT[CT]^[GA]AC HindII 623 GTTGAC GT[CT]^[GA]AC HindIII 3655 AAGCTT A^AGCTT MluI 2516 ACGCGT A^CGCGT MvnI 2517 CGCG CG^CG NheI 3910 GCTAGC G^CTAGC PshBI 4509 ATTAAT AT^TAAT RcaI 1879 TCATGA T^CATGA SbfI 2631 CCTGCAGG CCTGCA^GG SexAI 2239 ACCTGGT A^CC[AT]GGT SmaI 629 CCCGGG CCC^GGG SphI 4690 GCATGC GCATG^C Sse8387I 2631 CCTGCAGG CCTGCA^GG SspBI 55 TGTACA T^GTACA SspI 1552 AATATT AAT^ATT XmaCI 629 CCCGGG C^CCGGG XmaI 629 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### Asp700I 1518 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4078 GAAAGATTTC BbrPI 461 CACGTG CAC^GTG 2526 CACGTG BciVI 423 GTATCC GTATCC(6/5) 2520 GTATCC BglI 518 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1626 GCCTGCTAGGC BglII 434 AGATCT A^GATCT 4307 AGATCT BsmBI 2448 CGTCTC CGTCTC(1/5) 664 GAGACG BsmI 1758 GAATGC GAATGC(1/-1) 4411 GCATTC NcoI 2840 CCATGG C^CATGG 4760 CCATGG PaeR7I 2923 CTCGAG C^TCGAG 3093 CTCGAG PmaCI 461 CACGTG CAC^GTG 2526 CACGTG PmlI 461 CACGTG CAC^GTG 2526 CACGTG PvuII 2548 CAGCTG CAG^CTG 4827 CAGCTG StuI 1745 AGGCCT AGG^CCT 4242 AGGCCT XhoI 2923 CTCGAG C^TCGAG 3093 CTCGAG XmnI 1518 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4078 GAAAGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII