##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AviII BamHI BbeI
BbrPI BcgI BciVI BclI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BsrFI BssHII BstBI BstUI
CfoI Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
FbaI FseI FspI HaeII HhaI
HinP1I KasI KpnI KspI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 651 AACGTT AA^CGTT
AcyI 4316 GGCGTC G[GA]^CG[CT]C
AocI 5494 CCTCAGG CC^T[ACGT]AGG
ApaI 4825 GGGCCC GGGCC^C
AvaI 5402 CCCGAG C^[CT]CG[GA]G
BbvCI 5393 CCTCAGC CCTCAGC(-5/-2)
BglI 4782 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 48 AGATCT A^GATCT
BlpI 4766 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4766 GCTTAGC GC^T[ACGT]AGC
BsaAI 4136 CACGTA [CT]AC^GT[GA]
BsaHI 4316 GGCGTC G[GA]^CG[CT]C
BsmBI 5894 CGTCTC CGTCTC(1/5)
BsoBI 5402 CCCGAG C^[CT]CG[GA]G
BssSI 6091 CTCGTG CACGAG(-5/-1)
Bst1107I 5729 GTATAC GTA^TAC
BstAPI 5357 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3149 GGTGACC G^GT[ACGT]ACC
BstPI 3149 GGTGACC G^GT[ACGT]ACC
BstZ17I 5729 GTATAC GTA^TAC
Bsu36I 5494 CCTCAGG CC^T[ACGT]AGG
CelII 4766 GCTTAGC GC^T[ACGT]AGC
DrdI 849 GACAAAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5494 CCTCAGG CC^T[ACGT]AGG
EcoNI 3198 CCTGGTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3149 GGTGACC G^GT[ACGT]ACC
EcoRI 4334 GAATTC G^AATTC
HgaI 4317 GCGTC GACGC(5/10)
Hin1I 4316 GGCGTC G[GA]^CG[CT]C
HpaI 610 GTTAAC GTT^AAC
PflMI 1068 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 4208 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 651 AACGTT AA^CGTT
PvuII 299 CAGCTG CAG^CTG
SapI 3425 GAAGAGC GCTCTTC(1/4)
SexAI 3768 ACCAGGT A^CC[AT]GGT
SpeI 2297 ACTAGT A^CTAGT
StuI 4381 AGGCCT AGG^CCT
SwaI 2268 ATTTAAAT ATTT^AAAT
TaqI 937 TCGA T^CGA
TthHB8I 937 TCGA T^CGA
Van91I 1068 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1708 TCTAGA T^CTAGA
XcmI 3089 CCAGCAAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 3545 CTTAAG C^TTAAG
6229 CTTAAG
AseI 1917 ATTAAT AT^TAAT
6210 ATTAAT
AsnI 1917 ATTAAT AT^TAAT
6210 ATTAAT
AvrII 3115 CCTAGG C^CTAGG
3812 CCTAGG
BalI 1055 TGGCCA TGG^CCA
3821 TGGCCA
BanI 1780 GGTGCC G^G[CT][GA]CC
4155 GGCACC
BfrI 3545 CTTAAG C^TTAAG
6229 CTTAAG
BlnI 3115 CCTAGG C^CTAGG
3812 CCTAGG
BsaBI 2523 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4142 GATCCAAATC
EcoRV 36 GATATC GAT^ATC
5682 GATATC
EcoT22I 2464 ATGCAT ATGCA^T
4009 ATGCAT
MamI 2523 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4142 GATCCAAATC
MfeI 1963 CAATTG C^AATTG
5688 CAATTG
MluNI 1055 TGGCCA TGG^CCA
3821 TGGCCA
MscI 1055 TGGCCA TGG^CCA
3821 TGGCCA
MunI 1963 CAATTG C^AATTG
5688 CAATTG
NsiI 2464 ATGCAT ATGCA^T
4009 ATGCAT
PshBI 1917 ATTAAT AT^TAAT
6210 ATTAAT
PstI 4061 CTGCAG CTGCA^G
6420 CTGCAG
SphI 252 GCATGC GCATG^C
2462 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AspI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PleI
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I XhoII
XmnI