##################################
# NO CUT ENZYMES (   48 enzymes) #
##################################

AclI        AcsI        AflII       ApoI        AscI        
AseI        AsnI        AvrII       BamHI       BclI        
BfrI        BlnI        BsiWI       BspDI       BspHI       
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        DrdI        EcoRI       FbaI        
FseI        HpaI        MfeI        MluI        MunI        
NdeI        NheI        NotI        NruI        NspV        
PacI        PmeI        PshBI       Psp1406I    RcaI        
RsrII       SalI        SfuI        SspI        SwaI        
TfiI        Tsp509I     XbaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII        1376  GACGTC               GACGT^C
Acc65I       2949  GGTACC               G^GTACC
AccIII       1392  TCCGGA               T^CCGGA
AgeI         5461  ACCGGT               A^CCGGT
Asp700I      3236  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2949  GGTACC               G^GTACC
AspI          573  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        3711  CACGTG               CAC^GTG
BciVI        5645  GGATAC               GTATCC(6/5)
BglII        2597  AGATCT               A^GATCT
BseAI        1392  TCCGGA               T^CCGGA
BsmI         3311  GCATTC               GAATGC(1/-1)
Bsp1407I      181  TGTACA               T^GTACA
BspEI        1392  TCCGGA               T^CCGGA
BspLU11I     3187  ACATGT               A^CATGT
BsrGI         181  TGTACA               T^GTACA
BstAPI        725  GCACAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3137  GGTGACC              G^GT[ACGT]ACC
BstPI        3137  GGTGACC              G^GT[ACGT]ACC
EcoO65I      3137  GGTGACC              G^GT[ACGT]ACC
EcoRV        6212  GATATC               GAT^ATC
EcoT22I       425  ATGCAT               ATGCA^T
HindIII      4523  AAGCTT               A^AGCTT
KpnI         2949  GGTACC               GGTAC^C
KspI         3460  CCGCGG               CCGC^GG
MroI         1392  TCCGGA               T^CCGGA
NsiI          425  ATGCAT               ATGCA^T
PaeR7I       4927  CTCGAG               C^TCGAG
PflFI         573  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        5461  ACCGGT               A^CCGGT
PmaCI        3711  CACGTG               CAC^GTG
PmlI         3711  CACGTG               CAC^GTG
PshAI        4597  GACCGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         4252  CGATCG               CGAT^CG
SacII        3460  CCGCGG               CCGC^GG
SbfI         5540  CCTGCAGG             CCTGCA^GG
SexAI        5116  ACCAGGT              A^CC[AT]GGT
SnaBI        3762  TACGTA               TAC^GTA
SpeI         4860  ACTAGT               A^CTAGT
SphI         5797  GCATGC               GCATG^C
Sse8387I     5540  CCTGCAGG             CCTGCA^GG
SspBI         181  TGTACA               T^GTACA
Tth111I       573  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         4927  CTCGAG               C^TCGAG
XmnI         3236  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflIII       3187  ACATGT               A^C[GA][CT]GT
             3710  ACACGT              
AhdI         1562  GACGGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5505  GACATCCTGTC         
Aor51HI      4739  AGCGCT               AGC^GCT
             5551  AGCGCT              
AspEI        1562  GACGGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5505  GACATCCTGTC         
BbeI          686  GGCGCC               GGCGC^C
             5706  GGCGCC              
BbsI         1029  GAAGAC               GAAGAC(2/6)
             2812  GTCTTC              
BcgI         1427  CGAGTTTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3728  CGACTCCTGTGC        
BpuAI        1029  GAAGAC               GAAGAC(2/6)
             2812  GTCTTC              
BsaBI         699  GATGTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2420  GATGGTGATC          
BsrDI        1791  CATTGC               GCAATG(2/0)
             2000  CATTGC              
DraIII       2294  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4533  CACGAGGTG           
EagI         3040  CGGCCG               C^GGCCG
             4248  CGGCCG              
Eam1105I     1562  GACGGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5505  GACATCCTGTC         
EclXI        3040  CGGCCG               C^GGCCG
             4248  CGGCCG              
Eco47III     4739  AGCGCT               AGC^GCT
             5551  AGCGCT              
Eco52I       3040  CGGCCG               C^GGCCG
             4248  CGGCCG              
HincII       3612  GTTGAC               GT[CT]^[GA]AC
             3747  GTCAAC              
HindII       3612  GTTGAC               GT[CT]^[GA]AC
             3747  GTCAAC              
KasI          686  GGCGCC               G^GCGCC
             5706  GGCGCC              
MamI          699  GATGTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2420  GATGGTGATC          
NarI          686  GGCGCC               GG^CGCC
             5706  GGCGCC              
SapI          783  GAAGAGC              GCTCTTC(1/4)
             5324  GCTCTTC             
SfiI         3159  GGCCGCAAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             5765  GGCCACGGAGGCC       
SfoI          686  GGCGCC               GGC^GCC
             5706  GGCGCC              
SgrAI           9  CGCCGGCG             C[GA]^CCGG[CT]G
              691  CGCCGGTG            
SmaI         1639  CCCGGG               CCC^GGG
             1972  CCCGGG              
XmaCI        1639  CCCGGG               C^CCGGG
             1972  CCCGGG              
XmaI         1639  CCCGGG               C^CCGGG
             1972  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        Alw44I      AlwI        AlwNI       AocI        
ApaI        ApaLI       AspHI       AvaI        AvaII       
AviII       BalI        BanI        BanII       BbvCI       
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        Tru9I       
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XcmI        XhoII