################################## # NO CUT ENZYMES ( 48 enzymes) # ################################## AclI AcsI AflII ApoI AscI AseI AsnI AvrII BamHI BclI BfrI BlnI BsiWI BspDI BspHI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI EcoRI FbaI FseI HpaI MfeI MluI MunI NdeI NheI NotI NruI NspV PacI PmeI PshBI Psp1406I RcaI RsrII SalI SfuI SspI SwaI TfiI Tsp509I XbaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 1376 GACGTC GACGT^C Acc65I 2949 GGTACC G^GTACC AccIII 1392 TCCGGA T^CCGGA AgeI 5461 ACCGGT A^CCGGT Asp700I 3236 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2949 GGTACC G^GTACC AspI 573 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 3711 CACGTG CAC^GTG BciVI 5645 GGATAC GTATCC(6/5) BglII 2597 AGATCT A^GATCT BseAI 1392 TCCGGA T^CCGGA BsmI 3311 GCATTC GAATGC(1/-1) Bsp1407I 181 TGTACA T^GTACA BspEI 1392 TCCGGA T^CCGGA BspLU11I 3187 ACATGT A^CATGT BsrGI 181 TGTACA T^GTACA BstAPI 725 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3137 GGTGACC G^GT[ACGT]ACC BstPI 3137 GGTGACC G^GT[ACGT]ACC EcoO65I 3137 GGTGACC G^GT[ACGT]ACC EcoRV 6212 GATATC GAT^ATC EcoT22I 425 ATGCAT ATGCA^T HindIII 4523 AAGCTT A^AGCTT KpnI 2949 GGTACC GGTAC^C KspI 3460 CCGCGG CCGC^GG MroI 1392 TCCGGA T^CCGGA NsiI 425 ATGCAT ATGCA^T PaeR7I 4927 CTCGAG C^TCGAG PflFI 573 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 5461 ACCGGT A^CCGGT PmaCI 3711 CACGTG CAC^GTG PmlI 3711 CACGTG CAC^GTG PshAI 4597 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 4252 CGATCG CGAT^CG SacII 3460 CCGCGG CCGC^GG SbfI 5540 CCTGCAGG CCTGCA^GG SexAI 5116 ACCAGGT A^CC[AT]GGT SnaBI 3762 TACGTA TAC^GTA SpeI 4860 ACTAGT A^CTAGT SphI 5797 GCATGC GCATG^C Sse8387I 5540 CCTGCAGG CCTGCA^GG SspBI 181 TGTACA T^GTACA Tth111I 573 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 4927 CTCGAG C^TCGAG XmnI 3236 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflIII 3187 ACATGT A^C[GA][CT]GT 3710 ACACGT AhdI 1562 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5505 GACATCCTGTC Aor51HI 4739 AGCGCT AGC^GCT 5551 AGCGCT AspEI 1562 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5505 GACATCCTGTC BbeI 686 GGCGCC GGCGC^C 5706 GGCGCC BbsI 1029 GAAGAC GAAGAC(2/6) 2812 GTCTTC BcgI 1427 CGAGTTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3728 CGACTCCTGTGC BpuAI 1029 GAAGAC GAAGAC(2/6) 2812 GTCTTC BsaBI 699 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2420 GATGGTGATC BsrDI 1791 CATTGC GCAATG(2/0) 2000 CATTGC DraIII 2294 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 4533 CACGAGGTG EagI 3040 CGGCCG C^GGCCG 4248 CGGCCG Eam1105I 1562 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5505 GACATCCTGTC EclXI 3040 CGGCCG C^GGCCG 4248 CGGCCG Eco47III 4739 AGCGCT AGC^GCT 5551 AGCGCT Eco52I 3040 CGGCCG C^GGCCG 4248 CGGCCG HincII 3612 GTTGAC GT[CT]^[GA]AC 3747 GTCAAC HindII 3612 GTTGAC GT[CT]^[GA]AC 3747 GTCAAC KasI 686 GGCGCC G^GCGCC 5706 GGCGCC MamI 699 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2420 GATGGTGATC NarI 686 GGCGCC GG^CGCC 5706 GGCGCC SapI 783 GAAGAGC GCTCTTC(1/4) 5324 GCTCTTC SfiI 3159 GGCCGCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 5765 GGCCACGGAGGCC SfoI 686 GGCGCC GGC^GCC 5706 GGCGCC SgrAI 9 CGCCGGCG C[GA]^CCGG[CT]G 691 CGCCGGTG SmaI 1639 CCCGGG CCC^GGG 1972 CCCGGG XmaCI 1639 CCCGGG C^CCGGG 1972 CCCGGG XmaI 1639 CCCGGG C^CCGGG 1972 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII AviII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII