##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

AatII       AgeI        AscI        AseI        AsnI        
AviII       BcgI        BciVI       BclI        BglII       
BsaBI       BspDI       BssHII      Bst1107I    BstZ17I     
ClaI        CpoI        FbaI        FseI        FspI        
HpaI        KspI        MamI        MfeI        MluI        
MunI        NdeI        NotI        NruI        PacI        
PaeR7I      PflMI       PinAI       PmeI        PshAI       
PshBI       PvuI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SgrAI       SnaBI       
Sse8387I    SspI        StuI        SwaI        Van91I      
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        321  GGTACC               G^GTACC
AccI         1201  GTCTAC               GT^[AC][GT]AC
AccIII       6017  TCCGGA               T^CCGGA
AclI         1288  AACGTT               AA^CGTT
AhdI         5788  GACCCCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4359  GTGCAC               G^TGCAC
ApaI         5514  GGGCCC               GGGCC^C
ApaLI        4359  GTGCAC               G^TGCAC
Asp700I      4973  GAATACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       321  GGTACC               G^GTACC
AspEI        5788  GACCCCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        1245  GGATCC               G^GATCC
BseAI        6017  TCCGGA               T^CCGGA
BsiEI        5114  CGGCCG               CG[GA][CT]^CG
BsiWI        3657  CGTACG               C^GTACG
BsmBI        2350  GAGACG               CGTCTC(1/5)
Bsp1407I     3293  TGTACA               T^GTACA
BspEI        6017  TCCGGA               T^CCGGA
BspLU11I      385  ACATGT               A^CATGT
BsrGI        3293  TGTACA               T^GTACA
BstEII       3165  GGTGACC              G^GT[ACGT]ACC
BstPI        3165  GGTGACC              G^GT[ACGT]ACC
DraI         3715  TTTAAA               TTT^AAA
DraIII       1853  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         5114  CGGCCG               C^GGCCG
Eam1105I     5788  GACCCCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        5114  CGGCCG               C^GGCCG
Eco52I       5114  CGGCCG               C^GGCCG
EcoO65I      3165  GGTGACC              G^GT[ACGT]ACC
EcoRV        6091  GATATC               GAT^ATC
EcoT22I       224  ATGCAT               ATGCA^T
HindIII      6084  AAGCTT               A^AGCTT
KpnI          321  GGTACC               GGTAC^C
MroI         6017  TCCGGA               T^CCGGA
NheI         5070  GCTAGC               G^CTAGC
NsiI          224  ATGCAT               ATGCA^T
Psp1406I     1288  AACGTT               AA^CGTT
SpeI         1335  ACTAGT               A^CTAGT
SphI          671  GCATGC               GCATG^C
SspBI        3293  TGTACA               T^GTACA
XmnI         4973  GAATACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccII         648  CGCG                 CG^CG
             3854  CGCG                
AflII        4919  CTTAAG               C^TTAAG
             5080  CTTAAG              
Aor51HI      2571  AGCGCT               AGC^GCT
             5944  AGCGCT              
AspI         1552  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4002  GACAAGGTC           
AvrII        3435  CCTAGG               C^CTAGG
             4778  CCTAGG              
BalI         5702  TGGCCA               TGG^CCA
             5824  TGGCCA              
BbeI         4473  GGCGCC               GGCGC^C
             5779  GGCGCC              
BfrI         4919  CTTAAG               C^TTAAG
             5080  CTTAAG              
BglI         1404  GCCACACTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1501  GCCATGGTGGC         
BlnI         3435  CCTAGG               C^CTAGG
             4778  CCTAGG              
BlpI          708  GCTCAGC              GC^T[ACGT]AGC
             1375  GCTGAGC             
Bpu1102I      708  GCTCAGC              GC^T[ACGT]AGC
             1375  GCTGAGC             
BsmI         1032  GCATTC               GAATGC(1/-1)
             4672  GCATTC              
BspHI         614  TCATGA               T^CATGA
             3216  TCATGA              
BstAPI        990  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4950  GCATCCTGTGC         
BstBI        1702  TTCGAA               TT^CGAA
             1969  TTCGAA              
BstUI         648  CGCG                 CG^CG
             3854  CGCG                
CelII         708  GCTCAGC              GC^T[ACGT]AGC
             1375  GCTGAGC             
DrdI         1219  GACCAGTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2743  GACATTGGCGTC        
Eco47III     2571  AGCGCT               AGC^GCT
             5944  AGCGCT              
EcoRI        1804  GAATTC               G^AATTC
             2703  GAATTC              
KasI         4473  GGCGCC               G^GCGCC
             5779  GGCGCC              
MluNI        5702  TGGCCA               TGG^CCA
             5824  TGGCCA              
MscI         5702  TGGCCA               TGG^CCA
             5824  TGGCCA              
MvnI          648  CGCG                 CG^CG
             3854  CGCG                
NarI         4473  GGCGCC               GG^CGCC
             5779  GGCGCC              
NspV         1702  TTCGAA               TT^CGAA
             1969  TTCGAA              
PflFI        1552  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4002  GACAAGGTC           
RcaI          614  TCATGA               T^CATGA
             3216  TCATGA              
ScaI         1489  AGTACT               AGT^ACT
             5019  AGTACT              
SfoI         4473  GGCGCC               GGC^GCC
             5779  GGCGCC              
SfuI         1702  TTCGAA               TT^CGAA
             1969  TTCGAA              
Tth111I      1552  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4002  GACAAGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  132 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        ApoI        
AspHI       AvaI        AvaII       BanI        BanII       
BbrPI       BbsI        BbvCI       BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrDI       BsrFI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NaeI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PleI        PmaCI       
PmlI        PpuMI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
XmaCI       XmaI