##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AclI AflII Alw44I Aor51HI
ApaLI AscI Asp718I AviII AvrII
BclI BfrI BlnI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII BstXI ClaI CpoI DraI
EagI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KpnI MluI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PmeI Psp1406I PvuI RcaI RsrII
SalI SapI ScaI SfiI SgrAI
SnaBI SpeI SspBI StuI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 2900 GACGTC GACGT^C
AccIII 646 TCCGGA T^CCGGA
AflIII 4686 ACACGT A^C[GA][CT]GT
AgeI 1231 ACCGGT A^CCGGT
AhdI 2914 GACGTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3774 ATTAAT AT^TAAT
AsnI 3774 ATTAAT AT^TAAT
Asp700I 4404 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2914 GACGTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 531 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 5431 GGATCC G^GATCC
BbeI 2550 GGCGCC GGCGC^C
BbrPI 4687 CACGTG CAC^GTG
BciVI 3842 GTATCC GTATCC(6/5)
BsaAI 4687 CACGTG [CT]AC^GT[GA]
BsaBI 2664 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 646 TCCGGA T^CCGGA
BsiEI 2090 CGACCG CG[GA][CT]^CG
BsmBI 5655 GAGACG CGTCTC(1/5)
BspEI 646 TCCGGA T^CCGGA
BsrDI 4672 CATTGC GCAATG(2/0)
BssSI 5579 CACGAG CACGAG(-5/-1)
BstAPI 5568 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2058 TTCGAA TT^CGAA
DraIII 5819 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2022 GACTTCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2914 GACGTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KasI 2550 GGCGCC G^GCGCC
MamI 2664 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 646 TCCGGA T^CCGGA
NaeI 769 GCCGGC GCC^GGC
NarI 2550 GGCGCC GG^CGCC
NgoMIV 769 GCCGGC G^CCGGC
NspV 2058 TTCGAA TT^CGAA
PflFI 531 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1231 ACCGGT A^CCGGT
PmaCI 4687 CACGTG CAC^GTG
PmlI 4687 CACGTG CAC^GTG
PshBI 3774 ATTAAT AT^TAAT
SbfI 261 CCTGCAGG CCTGCA^GG
SfoI 2550 GGCGCC GGC^GCC
SfuI 2058 TTCGAA TT^CGAA
SphI 794 GCATGC GCATG^C
Sse8387I 261 CCTGCAGG CCTGCA^GG
SspI 3771 AATATT AAT^ATT
Tth111I 531 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4404 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 2550 GGCGCC G[GA]^CG[CT]C
2900 GACGTC
BanI 1806 GGCACC G^G[CT][GA]CC
2550 GGCGCC
BglI 21 GCCGAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
93 GCCGAGGGGGC
BglII 1138 AGATCT A^GATCT
3858 AGATCT
BsaHI 2550 GGCGCC G[GA]^CG[CT]C
2900 GACGTC
Bst1107I 2748 GTATAC GTA^TAC
6219 GTATAC
BstZ17I 2748 GTATAC GTA^TAC
6219 GTATAC
EarI 4390 CTCTTC CTCTTC(1/4)
2815 GAAGAG
HgaI 2889 GACGC GACGC(5/10)
5039 GCGTC
Hin1I 2550 GGCGCC G[GA]^CG[CT]C
2900 GACGTC
HincII 3353 GTCAAC GT[CT]^[GA]AC
4588 GTCAAC
HindII 3353 GTCAAC GT[CT]^[GA]AC
4588 GTCAAC
HindIII 996 AAGCTT A^AGCTT
4664 AAGCTT
Ksp632I 4390 CTCTTC CTCTTC(1/4)
2815 GAAGAG
KspI 71 CCGCGG CCGC^GG
5535 CCGCGG
MfeI 1207 CAATTG C^AATTG
6110 CAATTG
MunI 1207 CAATTG C^AATTG
6110 CAATTG
NspI 794 GCATGC [GA]CATG^[CT]
854 GCATGT
PflMI 2678 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5511 CCAGGGTTTGG
PshAI 2315 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5657 GACGTTTGTC
SacII 71 CCGCGG CCGC^GG
5535 CCGCGG
SexAI 532 ACCTGGT A^CC[AT]GGT
5244 ACCAGGT
Van91I 2678 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5511 CCAGGGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI