##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AccIII AflII AgeI Aor51HI AscI
AviII BbrPI BclI BfrI BsaBI
BseAI BsiWI BspEI BssHII BssSI
BstEII BstPI CpoI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FbaI FseI FspI KspI MamI
MluI MroI NaeI NcoI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI RsrII SacII SalI SbfI
SexAI SfiI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 246 GACGTC GACGT^C
Acc65I 123 GGTACC G^GTACC
AccII 3762 CGCG CG^CG
Asp718I 123 GGTACC G^GTACC
AspI 398 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2353 CCTAGG C^CTAGG
BalI 518 TGGCCA TGG^CCA
BamHI 5038 GGATCC G^GATCC
BciVI 1957 GTATCC GTATCC(6/5)
BlnI 2353 CCTAGG C^CTAGG
BlpI 2051 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2051 GCTCAGC GC^T[ACGT]AGC
BsaAI 5217 TACGTG [CT]AC^GT[GA]
BsaWI 2745 ACCGGA [AT]^CCGG[AT]
Bsp1407I 2730 TGTACA T^GTACA
BspLU11I 4108 ACATGT A^CATGT
BsrFI 1964 GCCGGT [GA]^CCGG[CT]
BsrGI 2730 TGTACA T^GTACA
Bst1107I 31 GTATAC GTA^TAC
BstBI 1912 TTCGAA TT^CGAA
BstUI 3762 CGCG CG^CG
BstXI 2206 CCACAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 31 GTATAC GTA^TAC
CelII 2051 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1964 GCCGGT [GA]^CCGG[CT]
DrdI 4509 GACATACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 5854 ATGCAT ATGCA^T
HpaI 407 GTTAAC GTT^AAC
KpnI 123 GGTACC GGTAC^C
MfeI 1594 CAATTG C^AATTG
MluNI 518 TGGCCA TGG^CCA
MscI 518 TGGCCA TGG^CCA
MunI 1594 CAATTG C^AATTG
MvnI 3762 CGCG CG^CG
NdeI 5964 CATATG CA^TATG
NsiI 5854 ATGCAT ATGCA^T
NspV 1912 TTCGAA TT^CGAA
PflFI 398 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 521 CCAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 4369 CGATCG CGAT^CG
SacI 4524 GAGCTC GAGCT^C
ScaI 1512 AGTACT AGT^ACT
SfuI 1912 TTCGAA TT^CGAA
SmaI 490 CCCGGG CCC^GGG
SphI 2056 GCATGC GCATG^C
SspBI 2730 TGTACA T^GTACA
SspI 4698 AATATT AAT^ATT
Tth111I 398 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 521 CCAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 490 CCCGGG C^CCGGG
XmaI 490 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AclI 1094 AACGTT AA^CGTT
1213 AACGTT
AocI 118 CCTGAGG CC^T[ACGT]AGG
1785 CCTGAGG
ApaI 826 GGGCCC GGGCC^C
3559 GGGCCC
AseI 5893 ATTAAT AT^TAAT
5990 ATTAAT
AsnI 5893 ATTAAT AT^TAAT
5990 ATTAAT
AvaI 490 CCCGGG C^[CT]CG[GA]G
966 CCCGAG
BbeI 2804 GGCGCC GGCGC^C
3955 GGCGCC
BfaI 2354 CTAG C^TAG
5735 CTAG
BglI 2140 GCCTGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3658 GCCTGGGCGGC
BglII 560 AGATCT A^GATCT
2892 AGATCT
BsaI 1241 GAGACC GGTCTC(1/5)
1463 GAGACC
BsiEI 989 CGGTCG CG[GA][CT]^CG
4369 CGATCG
BsmI 2180 GAATGC GAATGC(1/-1)
4451 GAATGC
BsoBI 490 CCCGGG C^[CT]CG[GA]G
966 CCCGAG
BspDI 1794 ATCGAT AT^CGAT
3642 ATCGAT
BspHI 2302 TCATGA T^CATGA
2838 TCATGA
Bsu36I 118 CCTGAGG CC^T[ACGT]AGG
1785 CCTGAGG
ClaI 1794 ATCGAT AT^CGAT
3642 ATCGAT
DraIII 888 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
3007 CACTATGTG
DsaI 976 CCGTGG C^C[GA][CT]GG
3799 CCGTGG
Eco81I 118 CCTGAGG CC^T[ACGT]AGG
1785 CCTGAGG
HgaI 547 GCGTC GACGC(5/10)
4455 GCGTC
HincII 407 GTTAAC GT[CT]^[GA]AC
4302 GTCAAC
HindII 407 GTTAAC GT[CT]^[GA]AC
4302 GTCAAC
KasI 2804 GGCGCC G^GCGCC
3955 GGCGCC
MaeI 2354 CTAG C^TAG
5735 CTAG
NarI 2804 GGCGCC GG^CGCC
3955 GGCGCC
PshBI 5893 ATTAAT AT^TAAT
5990 ATTAAT
Psp1406I 1094 AACGTT AA^CGTT
1213 AACGTT
RcaI 2302 TCATGA T^CATGA
2838 TCATGA
SfoI 2804 GGCGCC GGC^GCC
3955 GGCGCC
SmlI 2459 CTCAAG C^T[CT][GA]AG
4212 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AhdI AluI Alw44I AlwI
AlwNI ApaLI ApoI Asp700I AspEI
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BcgI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
Eam1105I EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI