##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AclI AflII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
AspI BbrPI BclI BfrI BspHI
BspLU11I Bst1107I BstBI BstZ17I DraI
Eam1105I EcoRV EcoT22I FbaI FseI
KspI MfeI MluI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacII ScaI SfuI SgrAI
SphI SspI SwaI Tth111I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 3490 GACGTC GACGT^C
Acc65I 5327 GGTACC G^GTACC
AccI 147 GTCGAC GT^[AC][GT]AC
Asp700I 2046 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5327 GGTACC G^GTACC
AviII 781 TGCGCA TGC^GCA
AvrII 4550 CCTAGG C^CTAGG
BciVI 3667 GGATAC GTATCC(6/5)
BglII 2437 AGATCT A^GATCT
BlnI 4550 CCTAGG C^CTAGG
BsmI 637 GAATGC GAATGC(1/-1)
BspDI 5582 ATCGAT AT^CGAT
BsrDI 5446 CATTGC GCAATG(2/0)
BssHII 307 GCGCGC G^CGCGC
BstEII 6301 GGTCACC G^GT[ACGT]ACC
BstPI 6301 GGTCACC G^GT[ACGT]ACC
ClaI 5582 ATCGAT AT^CGAT
CpoI 5294 CGGTCCG CG^G[AT]CCG
EcoO65I 6301 GGTCACC G^GT[ACGT]ACC
EcoRI 8 GAATTC G^AATTC
FspI 781 TGCGCA TGC^GCA
HindIII 1427 AAGCTT A^AGCTT
HpaI 2744 GTTAAC GTT^AAC
KpnI 5327 GGTACC GGTAC^C
NcoI 5436 CCATGG C^CATGG
NdeI 6385 CATATG CA^TATG
RsrII 5294 CGGTCCG CG^G[AT]CCG
SalI 147 GTCGAC G^TCGAC
SbfI 4037 CCTGCAGG CCTGCA^GG
SexAI 2003 ACCTGGT A^CC[AT]GGT
SfiI 4453 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 5372 CTCAAG C^T[CT][GA]AG
SnaBI 5020 TACGTA TAC^GTA
SpeI 431 ACTAGT A^CTAGT
Sse8387I 4037 CCTGCAGG CCTGCA^GG
XbaI 483 TCTAGA T^CTAGA
XmnI 2046 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 4851 TCCGGA T^CCGGA
5379 TCCGGA
AflIII 1504 ACACGT A^C[GA][CT]GT
5220 ACGTGT
AocI 423 CCTCAGG CC^T[ACGT]AGG
6295 CCTGAGG
BamHI 758 GGATCC G^GATCC
5412 GGATCC
BcgI 5271 CGAACCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
480 GCATCTAGATCG
BsaWI 4851 TCCGGA [AT]^CCGG[AT]
5379 TCCGGA
BseAI 4851 TCCGGA T^CCGGA
5379 TCCGGA
BsiWI 1074 CGTACG C^GTACG
5022 CGTACG
Bsp1407I 6097 TGTACA T^GTACA
6381 TGTACA
BspEI 4851 TCCGGA T^CCGGA
5379 TCCGGA
BsrGI 6097 TGTACA T^GTACA
6381 TGTACA
BssSI 5417 CACGAG CACGAG(-5/-1)
3273 CTCGTG
Bsu36I 423 CCTCAGG CC^T[ACGT]AGG
6295 CCTGAGG
DraIII 4935 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5590 CACCAAGTG
EagI 572 CGGCCG C^GGCCG
1324 CGGCCG
EclXI 572 CGGCCG C^GGCCG
1324 CGGCCG
Eco52I 572 CGGCCG C^GGCCG
1324 CGGCCG
Eco81I 423 CCTCAGG CC^T[ACGT]AGG
6295 CCTGAGG
EcoNI 2004 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4399 CCTCACCGAGG
MroI 4851 TCCGGA T^CCGGA
5379 TCCGGA
NspI 5508 ACATGC [GA]CATG^[CT]
5904 ACATGC
PflMI 1998 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4091 CCACCTGCTGG
SacI 2676 GAGCTC GAGCT^C
5860 GAGCTC
SspBI 6097 TGTACA T^GTACA
6381 TGTACA
Van91I 1998 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4091 CCACCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI