##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI Aor51HI AscI
Asp700I Asp718I AvaI AviII BamHI
BbeI BbrPI BciVI BfrI BlpI
Bpu1102I BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspEI BspHI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I CelII CfoI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoRV FseI FspI
HaeII HhaI HinP1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SfiI SfoI
SfuI SgrAI SmaI SspBI SspI
SwaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcyI 2956 GACGCC G[GA]^CG[CT]C
AflIII 448 ACATGT A^C[GA][CT]GT
Alw44I 135 GTGCAC G^TGCAC
AocI 214 CCTGAGG CC^T[ACGT]AGG
ApaLI 135 GTGCAC G^TGCAC
AseI 582 ATTAAT AT^TAAT
AsnI 582 ATTAAT AT^TAAT
AspI 3683 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3189 CCTAGG C^CTAGG
BanI 2528 GGCACC G^G[CT][GA]CC
BbvCI 1580 CCTCAGC CCTCAGC(-5/-2)
BcgI 3428 CGACCAACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2264 TGATCA T^GATCA
BcnI 3535 CCCGG CC^[GC]GG
BglI 2959 GCCCCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 905 AGATCT A^GATCT
BlnI 3189 CCTAGG C^CTAGG
BsaAI 2993 TACGTA [CT]AC^GT[GA]
BsaBI 2821 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2956 GACGCC G[GA]^CG[CT]C
BsaWI 1748 ACCGGA [AT]^CCGG[AT]
BspLU11I 448 ACATGT A^CATGT
BsrFI 3032 GCCGGT [GA]^CCGG[CT]
BstAPI 973 GCAAAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 214 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3032 GCCGGT [GA]^CCGG[CT]
DraIII 3382 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2783 CCATGG C^C[GA][CT]GG
Eco81I 214 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2076 ATGCAT ATGCA^T
FauI 3407 GCGGG CCCGC(4/6)
FbaI 2264 TGATCA T^GATCA
Hin1I 2956 GACGCC G[GA]^CG[CT]C
HincII 1053 GTTGAC GT[CT]^[GA]AC
HindII 1053 GTTGAC GT[CT]^[GA]AC
MamI 2821 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 3535 CCCGG CC^[GC]GG
NcoI 2783 CCATGG C^CATGG
NdeI 2108 CATATG CA^TATG
NsiI 2076 ATGCAT ATGCA^T
PflFI 3683 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2734 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 582 ATTAAT AT^TAAT
SapI 2595 GAAGAGC GCTCTTC(1/4)
SbfI 155 CCTGCAGG CCTGCA^GG
ScaI 2160 AGTACT AGT^ACT
SexAI 3319 ACCTGGT A^CC[AT]GGT
SnaBI 2993 TACGTA TAC^GTA
SpeI 1209 ACTAGT A^CTAGT
SphI 2475 GCATGC GCATG^C
Sse8387I 155 CCTGCAGG CCTGCA^GG
Tth111I 3683 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1500 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 1384 AAATTC [GA]^AATT[CT]
2274 AAATTC
AhdI 378 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1309 GACATCTTGTC
ApaI 1428 GGGCCC GGGCC^C
3165 GGGCCC
ApoI 1384 AAATTC [GA]^AATT[CT]
2274 AAATTC
AspEI 378 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1309 GACATCTTGTC
BalI 2097 TGGCCA TGG^CCA
2420 TGGCCA
BsaI 12 GAGACC GGTCTC(1/5)
2177 GAGACC
BsrDI 65 CATTGC GCAATG(2/0)
2765 CATTGC
BstEII 1911 GGTTACC G^GT[ACGT]ACC
3199 GGTGACC
BstPI 1911 GGTTACC G^GT[ACGT]ACC
3199 GGTGACC
DraI 868 TTTAAA TTT^AAA
2304 TTTAAA
Eam1105I 378 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1309 GACATCTTGTC
EcoO65I 1911 GGTTACC G^GT[ACGT]ACC
3199 GGTGACC
MluNI 2097 TGGCCA TGG^CCA
2420 TGGCCA
MscI 2097 TGGCCA TGG^CCA
2420 TGGCCA
NspI 448 ACATGT [GA]CATG^[CT]
2475 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspDI
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I ClaI DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII