##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AclI AflII AgeI Aor51HI AscI
AseI AsnI AviII BciVI BfrI
BsaBI BsiWI BspDI BspHI BssHII
Bst1107I BstBI BstZ17I ClaI DraI
Eco47III EcoRI EcoRV EcoT22I FspI
HpaI MamI MfeI MluI MunI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI SfuI SgrAI SnaBI
SpeI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 21 enzymes) #
###################################
AatII 6211 GACGTC GACGT^C
Acc65I 3385 GGTACC G^GTACC
AccIII 6217 TCCGGA T^CCGGA
Asp700I 7719 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3385 GGTACC G^GTACC
BclI 9762 TGATCA T^GATCA
BseAI 6217 TCCGGA T^CCGGA
BspEI 6217 TCCGGA T^CCGGA
CpoI 4507 CGGACCG CG^G[AT]CCG
FbaI 9762 TGATCA T^GATCA
FseI 2235 GGCCGGCC GGCCGG^CC
KpnI 3385 GGTACC GGTAC^C
MroI 6217 TCCGGA T^CCGGA
NdeI 5149 CATATG CA^TATG
PaeR7I 8742 CTCGAG C^TCGAG
PshAI 1446 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 4507 CGGACCG CG^G[AT]CCG
SalI 6208 GTCGAC G^TCGAC
SphI 3219 GCATGC GCATG^C
XhoI 8742 CTCGAG C^TCGAG
XmnI 7719 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 7881 AAATTC [GA]^AATT[CT]
9694 GAATTT
ApoI 7881 AAATTC [GA]^AATT[CT]
9694 GAATTT
AspI 1401 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
9057 GACACTGTC
BbrPI 1914 CACGTG CAC^GTG
3782 CACGTG
BcgI 8240 CGAGCACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
9889 GCAGAAGAATCG
BglII 2593 AGATCT A^GATCT
2935 AGATCT
BsaWI 838 ACCGGA [AT]^CCGG[AT]
6217 TCCGGA
BsiEI 2234 CGGCCG CG[GA][CT]^CG
7850 CGGCCG
EagI 2234 CGGCCG C^GGCCG
7850 CGGCCG
EclXI 2234 CGGCCG C^GGCCG
7850 CGGCCG
Eco52I 2234 CGGCCG C^GGCCG
7850 CGGCCG
HincII 5886 GTCAAC GT[CT]^[GA]AC
6208 GTCGAC
HindII 5886 GTCAAC GT[CT]^[GA]AC
6208 GTCGAC
HindIII 970 AAGCTT A^AGCTT
6085 AAGCTT
PflFI 1401 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
9057 GACACTGTC
PmaCI 1914 CACGTG CAC^GTG
3782 CACGTG
PmlI 1914 CACGTG CAC^GTG
3782 CACGTG
SapI 9446 GAAGAGC GCTCTTC(1/4)
8342 GCTCTTC
SbfI 1145 CCTGCAGG CCTGCA^GG
7532 CCTGCAGG
ScaI 7417 AGTACT AGT^ACT
8869 AGTACT
SexAI 5881 ACCAGGT A^CC[AT]GGT
6111 ACCTGGT
SmlI 8253 CTCAAG C^T[CT][GA]AG
8742 CTCGAG
Sse8387I 1145 CCTGCAGG CCTGCA^GG
7532 CCTGCAGG
Tth111I 1401 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
9057 GACACTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 164 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AhdI AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI AspEI
AspHI AvaI AvaII AvrII BalI
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SfoI SmaI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI