##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

AatII       Acc65I      AflII       Alw44I      ApaLI       
AscI        AseI        AsnI        Asp718I     AspI        
AviII       BfrI        BsaBI       BsiEI       BsiWI       
BspHI       Bst1107I    BstBI       BstZ17I     CpoI        
DraI        DrdI        EagI        EclXI       Eco52I      
EcoT22I     FspI        HindIII     HpaI        KpnI        
MamI        MfeI        MluI        MseI        MunI        
NheI        NotI        NsiI        NspV        PacI        
PflFI       PmeI        PshAI       PshBI       PvuI        
RcaI        RsrII       ScaI        SfiI        SfuI        
SnaBI       SpeI        SspI        SwaI        Tru9I       
Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI         3110  GTCGAC               GT^[AC][GT]AC
AclI         5366  AACGTT               AA^CGTT
AhdI         3037  GACCATGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      2634  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3037  GACCATGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI        5267  CACGTG               CAC^GTG
BciVI        2513  GTATCC               GTATCC(6/5)
BclI         5379  TGATCA               T^GATCA
BglII        3242  AGATCT               A^GATCT
BsaAI        5267  CACGTG               [CT]AC^GT[GA]
BsmI         5659  GAATGC               GAATGC(1/-1)
BspDI        1519  ATCGAT               AT^CGAT
BspLU11I     2882  ACATGT               A^CATGT
BstAPI        300  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3216  GGTCACC              G^GT[ACGT]ACC
BstPI        3216  GGTCACC              G^GT[ACGT]ACC
ClaI         1519  ATCGAT               AT^CGAT
DraIII       5233  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     3037  GACCATGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      3216  GGTCACC              G^GT[ACGT]ACC
FbaI         5379  TGATCA               T^GATCA
NdeI         1493  CATATG               CA^TATG
NruI          702  TCGCGA               TCG^CGA
PaeR7I       5289  CTCGAG               C^TCGAG
PflMI        2751  CCAGAAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        5267  CACGTG               CAC^GTG
PmlI         5267  CACGTG               CAC^GTG
Psp1406I     5366  AACGTT               AA^CGTT
SalI         3110  GTCGAC               G^TCGAC
SapI         3300  GCTCTTC              GCTCTTC(1/4)
SbfI         1185  CCTGCAGG             CCTGCA^GG
SexAI        3351  ACCTGGT              A^CC[AT]GGT
Sse8387I     1185  CCTGCAGG             CCTGCA^GG
Van91I       2751  CCAGAAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         5289  CTCGAG               C^TCGAG
XmnI         2634  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AflIII       2651  ACGTGT               A^C[GA][CT]GT
             2882  ACATGT              
AgeI          634  ACCGGT               A^CCGGT
             2553  ACCGGT              
AvrII          58  CCTAGG               C^CTAGG
             2619  CCTAGG              
BcgI         3112  CGACTGGTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3432  CGAGTCCATTGC        
BlnI           58  CCTAGG               C^CTAGG
             2619  CCTAGG              
BsmBI        3792  CGTCTC               CGTCTC(1/5)
             2648  GAGACG              
Bsp1407I     2885  TGTACA               T^GTACA
             4310  TGTACA              
BsrDI        3438  CATTGC               GCAATG(2/0)
             4776  GCAATG              
BsrGI        2885  TGTACA               T^GTACA
             4310  TGTACA              
BssHII         87  GCGCGC               G^CGCGC
              434  GCGCGC              
EcoRV        5518  GATATC               GAT^ATC
             5606  GATATC              
FseI         1822  GGCCGGCC             GGCCGG^CC
             4210  GGCCGGCC            
HincII       2953  GTTGAC               GT[CT]^[GA]AC
             3110  GTCGAC              
HindII       2953  GTTGAC               GT[CT]^[GA]AC
             3110  GTCGAC              
PinAI         634  ACCGGT               A^CCGGT
             2553  ACCGGT              
SgrAI         849  CGCCGGCG             C[GA]^CCGG[CT]G
             2552  CACCGGTG            
SphI         4002  GCATGC               GCATG^C
             5455  GCATGC              
SspBI        2885  TGTACA               T^GTACA
             4310  TGTACA              
StuI         2589  AGGCCT               AGG^CCT
             3881  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  146 enzymes) #
#########################################

AccII       AccIII      AciI        AcsI        AcyI        
AfaI        AluI        AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApoI        AspHI       AvaI        
AvaII       BalI        BamHI       BanI        BanII       
BbeI        BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspEI       BspMI       BsrBI       BsrFI       
BsrI        BssKI       BssSI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco47III    Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MroI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        SmlI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       XmaCI       
XmaI