################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AatII Acc65I AflII Alw44I ApaLI AscI AseI AsnI Asp718I AspI AviII BfrI BsaBI BsiEI BsiWI BspHI Bst1107I BstBI BstZ17I CpoI DraI DrdI EagI EclXI Eco52I EcoT22I FspI HindIII HpaI KpnI MamI MfeI MluI MseI MunI NheI NotI NsiI NspV PacI PflFI PmeI PshAI PshBI PvuI RcaI RsrII ScaI SfiI SfuI SnaBI SpeI SspI SwaI Tru9I Tth111I XbaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 3110 GTCGAC GT^[AC][GT]AC AclI 5366 AACGTT AA^CGTT AhdI 3037 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 2634 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3037 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 5267 CACGTG CAC^GTG BciVI 2513 GTATCC GTATCC(6/5) BclI 5379 TGATCA T^GATCA BglII 3242 AGATCT A^GATCT BsaAI 5267 CACGTG [CT]AC^GT[GA] BsmI 5659 GAATGC GAATGC(1/-1) BspDI 1519 ATCGAT AT^CGAT BspLU11I 2882 ACATGT A^CATGT BstAPI 300 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3216 GGTCACC G^GT[ACGT]ACC BstPI 3216 GGTCACC G^GT[ACGT]ACC ClaI 1519 ATCGAT AT^CGAT DraIII 5233 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 3037 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3216 GGTCACC G^GT[ACGT]ACC FbaI 5379 TGATCA T^GATCA NdeI 1493 CATATG CA^TATG NruI 702 TCGCGA TCG^CGA PaeR7I 5289 CTCGAG C^TCGAG PflMI 2751 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 5267 CACGTG CAC^GTG PmlI 5267 CACGTG CAC^GTG Psp1406I 5366 AACGTT AA^CGTT SalI 3110 GTCGAC G^TCGAC SapI 3300 GCTCTTC GCTCTTC(1/4) SbfI 1185 CCTGCAGG CCTGCA^GG SexAI 3351 ACCTGGT A^CC[AT]GGT Sse8387I 1185 CCTGCAGG CCTGCA^GG Van91I 2751 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 5289 CTCGAG C^TCGAG XmnI 2634 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 2651 ACGTGT A^C[GA][CT]GT 2882 ACATGT AgeI 634 ACCGGT A^CCGGT 2553 ACCGGT AvrII 58 CCTAGG C^CTAGG 2619 CCTAGG BcgI 3112 CGACTGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3432 CGAGTCCATTGC BlnI 58 CCTAGG C^CTAGG 2619 CCTAGG BsmBI 3792 CGTCTC CGTCTC(1/5) 2648 GAGACG Bsp1407I 2885 TGTACA T^GTACA 4310 TGTACA BsrDI 3438 CATTGC GCAATG(2/0) 4776 GCAATG BsrGI 2885 TGTACA T^GTACA 4310 TGTACA BssHII 87 GCGCGC G^CGCGC 434 GCGCGC EcoRV 5518 GATATC GAT^ATC 5606 GATATC FseI 1822 GGCCGGCC GGCCGG^CC 4210 GGCCGGCC HincII 2953 GTTGAC GT[CT]^[GA]AC 3110 GTCGAC HindII 2953 GTTGAC GT[CT]^[GA]AC 3110 GTCGAC PinAI 634 ACCGGT A^CCGGT 2553 ACCGGT SgrAI 849 CGCCGGCG C[GA]^CCGG[CT]G 2552 CACCGGTG SphI 4002 GCATGC GCATG^C 5455 GCATGC SspBI 2885 TGTACA T^GTACA 4310 TGTACA StuI 2589 AGGCCT AGG^CCT 3881 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 146 enzymes) # ######################################### AccII AccIII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI ApaI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI